| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 156, Issue 9 3118-3132, Copyright © 1996 by American Association of Immunologists
ARTICLES |
MC Bergman, JF Attrep, AC Grammer and PE Lipsky
Harold C. Simmons Arthritis Research Center, University of Texas Southwestern Medical Center, Dallas 75235, USA.
The effect of ligation of CD40 on the proliferation and Ig secretion of a battery of human Ig-secreting hybridomas was examined to determine the regulatory activity of this surface molecule on B cells after initial activation. B cell hybridomas were generated by fusing activated peripheral blood B cells with SPAZ-4, a non-Ig-secreting fusion partner, and were cloned before analysis. All hybridomas expressed CD40 comparably. These hybridomas were stimulated with either recombinant baculovirus-expressed membrane-bound CD40L or a soluble murine CD40L/CD8 construct in the presence or the absence of various cytokines. Concentrations of CD40L that saturated 40 to 100% of CD40 induced initial homotypic aggregation followed by Fas (CD95)- independent apoptosis, with resultant decreases in growth and Ig secretion. Concentrations of CD40L that saturated 15 to 25% of CD40 also stimulated aggregation of all hybridomas. However, proliferation and Ig secretion of 9 of 13 IgM-secreting hybridomas, but none of 14 IgG- or IgA-secreting hybridomas, were enhanced by these concentrations of CD40L. These responses were independent of interactions mediated by the adhesion pair CD1la/CD18-CD54. These results indicate that the impact of CD40 ligation on human Ig-secreting hybridomas varies with the extent of CD40 engagement and depending on whether the hybridoma derived from an activated B cell that had previously undergone switch recombination.
This article has been cited by other articles:
|
|
 |
|
  M. A. Kilmon, N. J. Wagner, A. L. Garland, L. Lin, K. Aviszus, L. J. Wysocki, and B. J. Vilen Macrophages prevent the differentiation of autoreactive B cells by secreting CD40 ligand and interleukin-6 Blood, September 1, 2007; 110(5): 1595 - 1602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hausl, R. U. Ahmad, H. P. Schwarz, E. M. Muchitsch, P. L. Turecek, F. Dorner, and B. M. Reipert Preventing restimulation of memory B cells in hemophilia A: a potential new strategy for the treatment of antibody-dependent immune disorders Blood, July 1, 2004; 104(1): 115 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Bharti, Y. Takada, S. Shishodia, and B. B. Aggarwal Evidence That Receptor Activator of Nuclear Factor (NF)-{kappa}B Ligand Can Suppress Cell Proliferation and Induce Apoptosis through Activation of a NF-{kappa}B-independent and TRAF6-dependent Mechanism J. Biol. Chem., February 13, 2004; 279(7): 6065 - 6076. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. O. Lee, L. Haynes, S. M. Eaton, S. L. Swain, and T. D. Randall The Biological Outcome of CD40 Signaling Is Dependent on the Duration of CD40 Ligand Expression: Reciprocal Regulation by Interleukin (IL)-4 and IL-12 J. Exp. Med., September 2, 2002; 196(5): 693 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Challa, A. G. Eliopoulos, M. J. Holder, A. S. Burguete, J. D. Pound, A. Chamba, G. Grafton, R. J. Armitage, C. D. Gregory, H. Martinez-Valdez, et al. Population depletion activates autonomous CD154-dependent survival in biopsylike Burkitt lymphoma cells Blood, May 1, 2002; 99(9): 3411 - 3418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Martinez-Barnetche, V. Madrid-Marina, R. A. Flavell, and J. Moreno Does CD40 Ligation Induce B Cell Negative Selection? J. Immunol., February 1, 2002; 168(3): 1042 - 1049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Majlessi and G. Bordenave Role of CD40 in a T Cell-Mediated Negative Regulation of Ig Production J. Immunol., January 15, 2001; 166(2): 841 - 847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Liang, C. F. Reich, D. S. Pisetsky, and P. E. Lipsky The Role of Cell Surface Receptors in the Activation of Human B Cells by Phosphorothioate Oligonucleotides J. Immunol., August 1, 2000; 165(3): 1438 - 1445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Posner, L. A. Cavacini, M. P. Upton, K. C. Tillman, E. R. Gornstein, and C. M. Norris Jr. Surface Membrane-expressed CD40 Is Present on Tumor Cells from Squamous Cell Cancer of the Head and Neck in Vitro and in Vivo and Regulates Cell Growth in Tumor Cell Lines Clin. Cancer Res., August 1, 1999; 5(8): 2261 - 2270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Leveille, H. Zekki, R. Al-Daccak, and W. Mourad CD40- and HLA-DR-mediated cell death pathways share a lot of similarities but differ in their use of ADP-ribosyltransferase activities Int. Immunol., May 1, 1999; 11(5): 719 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. V. Henriquez, E. Floettmann, M. Salmon, M. Rowe, and A. B. Rickinson Differential Responses to CD40 Ligation Among Burkitt Lymphoma Lines That Are Uniformly Responsive to Epstein-Barr Virus Latent Membrane Protein 1 J. Immunol., March 15, 1999; 162(6): 3298 - 3307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Grammer, J. L. Swantek, R. D. McFarland, Y. Miura, T. Geppert, and P. E. Lipsky TNF Receptor-Associated Factor-3 Signaling Mediates Activation of p38 and Jun N-Terminal Kinase, Cytokine Secretion, and Ig Production Following Ligation of CD40 on Human B Cells J. Immunol., August 1, 1998; 161(3): 1183 - 1193. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
