The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Salkowski, C.
Right arrow Articles by Vogel, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salkowski, C.
Right arrow Articles by Vogel, S.

The Journal of Immunology, Vol 156, Issue 9 3107-3110, Copyright © 1996 by American Association of Immunologists

CUTTING EDGE

Differential dysregulation of nitric oxide production in macrophages with targeted disruptions in IFN regulatory factor-1 and -2 genes

CA Salkowski, SA Barber, GR Detore and SN Vogel
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.

Recent studies have reported that IFN regulatory factor-1 (IRF-1) regulates nitric oxide (NO.) production in murine macrophages (M phi). Since IRF-2 recognizes the same consensus sequence as IRF-1, we postulated that IRF-2 may also regulate NO.. Therefore, the ability of M phi from INF-2 homozygous (IRF-2-/-) and heterozygous (IRF-2+/-) knockout mice to produce NO. following LPS and/or IFN-gamma stimulation was compared with the responses of IRF-1-/-, IRF-1+/-, and C57BL/6+/+ M phi. IRF-2-/- M phi produced less LPS-induced NO2- than IRF-2+/- or C57BL/6 M phi. LPS and IFN-gamma synergized to increase NO2- production from IRF-2-/- M phi to approximately 50% of IRF-2+/- and C57BL/6 levels. Unexpectedly, IRF-2-/-, IRF-2+/- and C57BL/6 M phi produced comparable levels of inducible NO synthase mRNA in response to treatment with LPS and IFN-gamma. IRF-1-/- M phi produced barely detectable NO2- and low, but detectable, inducible NO. synthase mRNA in response to IFN-gamma and LPS. These results indicate that IRF-1 and IRF-2 differ in their mechanism of NO. regulation and that IRF-2 regulates inducible NO. synthase post-transcriptionally.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
C. Gao, H. Guo, Z. Mi, P. Y. Wai, and P. C. Kuo
Transcriptional Regulatory Functions of Heterogeneous Nuclear Ribonucleoprotein-U and -A/B in Endotoxin-Mediated Macrophage Expression of Osteopontin
J. Immunol., July 1, 2005; 175(1): 523 - 530.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Cuesta, C. A. Salkowski, K. E. Thomas, and S. N. Vogel
Regulation of Lipopolysaccharide Sensitivity by IFN Regulatory Factor-2
J. Immunol., June 1, 2003; 170(11): 5739 - 5747.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Ko, A. Gendron-Fitzpatrick, T. A. Ficht, and G. A. Splitter
Virulence Criteria for Brucella abortus Strains as Determined by Interferon Regulatory Factor 1-Deficient Mice
Infect. Immun., December 1, 2002; 70(12): 7004 - 7012.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. L. McCartney-Francis and S. M. Wahl
Dysregulation of IFN-{gamma} Signaling Pathways in the Absence of TGF-{beta}1
J. Immunol., November 15, 2002; 169(10): 5941 - 5947.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
X. Xu, K. Sumita, C. Feng, X. Xiong, H. Shen, S. Maruyama, M. Kanoh, and Y. Asano
Down-Regulation of IL-12 p40 Gene in Plasmodium berghei-Infected Mice
J. Immunol., July 1, 2001; 167(1): 235 - 241.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
E. D. Chan, K. R. Morris, J. T. Belisle, P. Hill, L. K. Remigio, P. J. Brennan, and D. W. H. Riches
Induction of Inducible Nitric Oxide Synthase-NO{middle dot} by Lipoarabinomannan of Mycobacterium tuberculosis Is Mediated by MEK1-ERK, MKK7-JNK, and NF-{kappa}B Signaling Pathways
Infect. Immun., April 1, 2001; 69(4): 2001 - 2010.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Kröger, D. Ortmann, T. U. Krohne, L. Mohr, H. E. Blum, H. Hauser, and M. Geissler
Growth Suppression of the Hepatocellular Carcinoma Cell Line Hepa1-6 by an Activatable Interferon Regulatory Factor-1 in Mice
Cancer Res., March 1, 2001; 61(6): 2609 - 2617.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
C. A. Salkowski, K. E. Thomas, M. J. Cody, and S. N. Vogel
Impaired IFN-{gamma} Production in IFN Regulatory Factor-1 Knockout Mice During Endotoxemia Is Secondary to a Loss of Both IL-12 and IL-12 Receptor Expression
J. Immunol., October 1, 2000; 165(7): 3970 - 3977.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
J. C. G. Blanco, C. Contursi, C. A. Salkowski, D. L. DeWitt, K. Ozato, and S. N. Vogel
Interferon Regulatory Factor (IRF)-1 and IRF-2 Regulate Interferon {gamma}-dependent Cyclooxygenase 2 Expression
J. Exp. Med., June 19, 2000; 191(12): 2131 - 2144.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W.-S. Yeow, W.-C. Au, Y.-T. Juang, C. D. Fields, C. L. Dent, D. R. Gewert, and P. M. Pitha
Reconstitution of Virus-mediated Expression of Interferon alpha Genes in Human Fibroblast Cells by Ectopic Interferon Regulatory Factor-7
J. Biol. Chem., February 25, 2000; 275(9): 6313 - 6320.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Q.-P. Liu, K. Fruit, J. Ward, and P. H. Correll
Negative Regulation of Macrophage Activation in Response to IFN-{gamma} and Lipopolysaccharide by the STK/RON Receptor Tyrosine Kinase
J. Immunol., December 15, 1999; 163(12): 6606 - 6613.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. A. Salkowski, K. Kopydlowski, J. Blanco, M. J. Cody, R. McNally, and S. N. Vogel
IL-12 Is Dysregulated in Macrophages from IRF-1 and IRF-2 Knockout Mice
J. Immunol., August 1, 1999; 163(3): 1529 - 1536.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. S.-P. Tan, C. Feng, Y. Asano, and A. U. Kara
Altered Immune Response of Interferon Regulatory Factor 1-Deficient Mice against Plasmodium berghei Blood-Stage Malaria Infection
Infect. Immun., May 1, 1999; 67(5): 2277 - 2283.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
C. Iadecola, C. A. Salkowski, F. Zhang, T. Aber, M. Nagayama, S. N. Vogel, and M. Elizabeth Ross
The Transcription Factor Interferon Regulatory Factor 1 Is Expressed after Cerebral Ischemia and Contributes to Ischemic Brain Injury
J. Exp. Med., February 15, 1999; 189(4): 719 - 727.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. D. Chan, B. W. Winston, S.-T. Uh, M. W. Wynes, D. M. Rose, and D. W. H. Riches
Evaluation of the Role of Mitogen-Activated Protein Kinases in the Expression of Inducible Nitric Oxide Synthase by IFN-{gamma} and TNF-{alpha} in Mouse Macrophages
J. Immunol., January 1, 1999; 162(1): 415 - 422.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
F. Facchetti, W. Vermi, S. Fiorentini, M. Chilosi, A. Caruso, M. Duse, L. D. Notarangelo, and R. Badolato
Expression of Inducible Nitric Oxide Synthase in Human Granulomas and Histiocytic Reactions
Am. J. Pathol., January 1, 1999; 154(1): 145 - 152.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. J. Gao, M. B. Filla, M. J. Fultz, S. N. Vogel, S. W. Russell, and W. J. Murphy
Autocrine/Paracrine IFN-{alpha}{beta} Mediates the Lipopolysaccharide-Induced Activation of Transcription Factor Stat1{alpha} in Mouse Macrophages: Pivotal Role of Stat1{alpha} in Induction of the Inducible Nitric Oxide Synthase Gene
J. Immunol., November 1, 1998; 161(9): 4803 - 4810.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W.-C. Au, P. A. Moore, D. W. LaFleur, B. Tombal, and P. M. Pitha
Characterization of the Interferon Regulatory Factor-7 and Its Potential Role in the Transcription Activation of Interferon A Genes
J. Biol. Chem., October 30, 1998; 273(44): 29210 - 29217.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Lakics and S. N. Vogel
Lipopolysaccharide and Ceramide Use Divergent Signaling Pathways to Induce Cell Death in Murine Macrophages
J. Immunol., September 1, 1998; 161(5): 2490 - 2500.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Lopez-Collazo, S. Hortelano, A. Rojas, and L. Bosca
Triggering of Peritoneal Macrophages with IFN-{alpha}/{beta} Attenuates the Expression of Inducible Nitric Oxide Synthase Through a Decrease in NF-{kappa}B Activation
J. Immunol., March 15, 1998; 160(6): 2889 - 2895.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. L. Perry, K. Feilzer, and H. D. Caldwell
Neither Interleukin-6 nor Inducible Nitric Oxide Synthase Is Required for Clearance of Chlamydia trachomatis from the Murine Genital Tract Epithelium
Infect. Immun., March 1, 1998; 66(3): 1265 - 1269.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
N. A. Giese, L. Gabriele, T. M. Doherty, D. M. Klinman, L. Tadesse-Heath, C. Contursi, S. L. Epstein, and H. C. Morse III
Interferon (IFN) Consensus Sequence-binding Protein, a Transcription Factor of the IFN Regulatory Factor Family, Regulates Immune Responses In Vivo through Control of Interleukin 12 Expression
J. Exp. Med., November 3, 1997; 186(9): 1535 - 1546.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.