| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 156, Issue 8 2783-2791, Copyright © 1996 by American Association of Immunologists
ARTICLES |
M Natesan, Z Razi-Wolf and H Reiser
Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
We have examined the capacity of murine B7-1 and B7-2 to costimulate the production of IL-4 by murine CD4+ T lymphocytes. Cloned and freshly isolated T cells were incubated with the anti-CD3 mAb 145-2C11 in the presence of Chinese hamster ovary (CHO) cells that stably express murine B7-1 and B7-2 at comparable levels. IL-4 protein levels were measured in culture supernatants by the CT.4S bioassay, and levels of IL-4 mRNA were determined by semiquantitative reverse transcription- PCR. Both B7-1- and B7-2-transfected CHO cells, but not CHO control transfectants, were able to costimulate IL-4 production. Similarly, both B7-1 and B7-2 could up-regulate IFN-gamma mRNA levels. Cell fractionation experiments on freshly isolated CD4+ T lymphocytes revealed that the costimulatory potential of B7-1 and B7-2 for IL-4 production was restricted to CD44high T cells, i.e., the subpopulation that contains recently activated and memory cells. CD44low, naive CD4+ T lymphocytes, could only be induced to produce IL-4 by repeated stimulation with B7 transfectants. In summary, we have not detected qualitative differences in the capacities of murine B7-1 and B7-2 to induce IL-4 production. The results of our experiments, therefore, argue against the recent hypothesis that precursor Th cells are directed toward the Th2 phenotype by B7-2 and toward the Th1 phenotype by B7-1.
This article has been cited by other articles:
|
|
 |
|
  L. G. Thebeau, S. P. Vagvala, Y. M. Wong, and L. A. Morrison B7 Costimulation Molecules Expressed from the Herpes Simplex Virus 2 Genome Rescue Immune Induction in B7-Deficient Mice J. Virol., November 15, 2007; 81(22): 12200 - 12209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Corydon, A. Haagerup, T. G. Jensen, H. G. Binderup, M. S. Petersen, K. Kaltoft, J. Vestbo, T. A. Kruse, and A. D. Borglum A functional CD86 polymorphism associated with asthma and related allergic disorders J. Med. Genet., August 1, 2007; 44(8): 509 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kallinich, K. C. Beier, U. Wahn, P. Stock, and E. Hamelmann T-cell co-stimulatory molecules: their role in allergic immune reactions Eur. Respir. J., June 1, 2007; 29(6): 1246 - 1255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Zhang, M. Martin, Q.-B. Yang, S. M. Michalek, and J. Katz Role of B7 Costimulatory Molecules in Immune Responses and T-Helper Cell Differentiation in Response to Recombinant HagB from Porphyromonas gingivalis Infect. Immun., February 1, 2004; 72(2): 637 - 644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Wille, E. N. Villegas, L. Craig, R. Peach, and C. A. Hunter Contribution of Interleukin-12 (IL-12) and the CD28/B7 and CD40/CD40 Ligand Pathways to the Development of a Pathological T-Cell Response in IL-10-Deficient Mice Infect. Immun., December 1, 2002; 70(12): 6940 - 6947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Campbell, T. Komata, and A. Kelso CD4 Ligation Promotes the IL-4-Independent Development of IL-4-Producing Clones from Naive CD4+ T Cells J. Immunol., November 15, 2001; 167(10): 5610 - 5619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. M. Broeren, G. S. Gray, B. M. Carreno, and C. H. June Costimulation Light: Activation of CD4+ T Cells with CD80 or CD86 Rather Than Anti-CD28 Leads to a Th2 Cytokine Profile J. Immunol., December 15, 2000; 165(12): 6908 - 6914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Cain and G. S. Deepe Jr. Interleukin-12 Neutralization Alters Lung Inflammation and Leukocyte Expression of CD80, CD86, and Major Histocompatibility Complex Class II in Mice Infected with Histoplasma capsulatum Infect. Immun., April 1, 2000; 68(4): 2069 - 2076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Martin-Fontecha, M. Moro, M. C. Crosti, F. Veglia, G. Casorati, and P. Dellabona Vaccination with Mouse Mammary Adenocarcinoma Cells Coexpressing B7-1 (CD80) and B7-2 (CD86) Discloses the Dominant Effect of B7-1 in the Induction of Antitumor Immunity J. Immunol., January 15, 2000; 164(2): 698 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Agranovich, D. E. Scott, D. Terle, K. Lee, and B. Golding Down-Regulation of Th2 Responses by Brucella abortus, a Strong Th1 Stimulus, Correlates with Alterations in the B7.2-CD28 Pathway Infect. Immun., September 1, 1999; 67(9): 4418 - 4426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Rogers and M. Croft Peptide Dose, Affinity, and Time of Differentiation Can Contribute to the Th1/Th2 Cytokine Balance J. Immunol., August 1, 1999; 163(3): 1205 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Roufosse, L. Schandene, C. Sibille, B. Kennes, A. Efira, E. Cogan, and M. Goldman T-Cell Receptor-Independent Activation of Clonal Th2 Cells Associated With Chronic Hypereosinophilia Blood, August 1, 1999; 94(3): 994 - 1002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamamoto, H. Kiyono, S. Yamamoto, E. Batanero, M.-N. Kweon, S. Otake, M. Azuma, Y. Takeda, and J. R. McGhee Direct Effects on Antigen-Presenting Cells and T Lymphocytes Explain the Adjuvanticity of a Nontoxic Cholera Toxin Mutant J. Immunol., June 15, 1999; 162(12): 7015 - 7021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Elloso and P. Scott Expression and Contribution of B7-1 (CD80) and B7-2 (CD86) in the Early Immune Response to Leishmania major Infection J. Immunol., June 1, 1999; 162(11): 6708 - 6715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Aune, A. L. Mora, S. Kim, M. Boothby, and A. H. Lichtman Costimulation Reverses the Defect in IL-2 But Not Effector Cytokine Production by T Cells with Impaired I{kappa}B{alpha} Degradation J. Immunol., May 15, 1999; 162(10): 5805 - 5812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Greenwald, J. F. Urban, M. J. Ekkens, S.-J. Chen, D. Nguyen, H. Fang, F. D. Finkelman, A. H. Sharpe, and W. C. Gause B7-2 Is Required for the Progression But Not the Initiation of the Type 2 Immune Response to a Gastrointestinal Nematode Parasite J. Immunol., April 1, 1999; 162(7): 4133 - 4139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Maxwell, J. D. Campbell, C. H. Kim, and A. T. Vella CD40 Activation Boosts T Cell Immunity In Vivo by Enhancing T Cell Clonal Expansion and Delaying Peripheral T Cell Deletion J. Immunol., February 15, 1999; 162(4): 2024 - 2034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Youn, J. Chen, S. Goenka, M. A. Aronica, A. L. Mora, V. Correa, J. R. Sheller, and M. Boothby In Vivo Function of an Interleukin 2 Receptor beta Chain (IL-2Rbeta )/IL-4Ralpha Cytokine Receptor Chimera Potentiates Allergic Airway Disease J. Exp. Med., November 16, 1998; 188(10): 1803 - 1816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Fields, R. J. Finch, G. S. Gray, R. Zollner, J. L. Thomas, K. Sturmhoefel, K. Lee, S. Wolf, T. F. Gajewski, and F. W. Fitch B7.1 Is a Quantitatively Stronger Costimulus Than B7.2 in the Activation of Naive CD8+ TCR-Transgenic T Cells J. Immunol., November 15, 1998; 161(10): 5268 - 5275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guerder, E. E. Eynon, and R. A. Flavell Autoimmunity Without Diabetes in Transgenic Mice Expressing {beta} Cell-Specific CD86, But Not CD80: Parameters that Trigger Progression to Diabetes J. Immunol., September 1, 1998; 161(5): 2128 - 2140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Scheipers and H. Reiser Fas-independent death of activated CD4+ T lymphocytes induced by CTLA-4 crosslinking PNAS, August 18, 1998; 95(17): 10083 - 10088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Issazadeh, V. Navikas, M. Schaub, M. Sayegh, and S. Khoury Kinetics of Expression of Costimulatory Molecules and Their Ligands in Murine Relapsing Experimental Autoimmune Encephalomyelitis In Vivo J. Immunol., August 1, 1998; 161(3): 1104 - 1112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Jirapongsananuruk, M. F. Hofer, A. E. Trumble, D. A. Norris, and D. Y. M. Leung Enhanced Expression of B7.2 (CD86) in Patients with Atopic Dermatitis: A Potential Role in the Modulation of IgE Synthesis J. Immunol., May 1, 1998; 160(9): 4622 - 4627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. T. Maecker, M.-S. Do, and S. Levy CD81 on B cells promotes interleukin 4 secretion and antibody production during T helper type 2 immune responses PNAS, March 3, 1998; 95(5): 2458 - 2462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Keane-Myers, W. C. Gause, F. D. Finkelman, X.-d. Xhou, and M. Wills-Karp Development of Murine Allergic Asthma Is Dependent Upon B7-2 Costimulation J. Immunol., January 15, 1998; 160(2): 1036 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Jeannin, Y. Delneste, S. Lecoanet-Henchoz, J.-F. Gauchat, J. Ellis, and J.-Y. Bonnefoy CD86 (B7-2) on Human B Cells. A FUNCTIONAL ROLE IN PROLIFERATION AND SELECTIVE DIFFERENTIATION INTO IgE- AND IgG4-PRODUCING CELLS J. Biol. Chem., June 20, 1997; 272(25): 15613 - 15619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Reiser and M. J. Stadecker Costimulatory B7 Molecules in the Pathogenesis of Infectious and Autoimmune Diseases N. Engl. J. Med., October 31, 1996; 335(18): 1369 - 1377. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
