The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krco, C. J.
Right arrow Articles by David, C. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krco, C. J.
Right arrow Articles by David, C. S.

The Journal of Immunology, Vol 156, Issue 8 2761-2768, Copyright © 1996 by American Association of Immunologists

ARTICLES

Characterization of the antigenic structure of human type II collagen

CJ Krco, J Pawelski, J Harders, D McCormick, M Griffiths, HS Luthra and CS David
Department of Immunology, Mayo Clinic, Rochester, MN 55905, USA.

A series of 101 peptides each 20 amino acids in length (10-residue overlap) spanning the helical portion of the mature alpha-chain of human type II collagen (CII) was synthesized. DBA/1 (H-2q) mice were immunized with individual peptides, and draining lymph node cells were challenged in vitro. Strong responses were measured to three peptides: peptide I (residues 74-93), peptide 14 (residues 254-273), and peptide 81 (residues 924-943). B10.Q (H-2q) mice were responsive to peptides I and 81 but not to peptide 14. B10.RIII (H-2r) mice, which are resistant to arthritis induction following immunization with human CII, were unresponsive to peptides I, 14, and 81. Using single amino acid truncated peptides, we determined minimal immunostimulatory lengths for peptides I and 81. Residues critical to antigenicity were identified by introducing alanine and glycine substitutions into minimal length immunostimulatory peptides. The determinants within peptides I and 81 are 100% homologous to mouse CII and are autoantigens. Peptide 81 has homology to viral proteins. Peptide 14 is 90% homologous to mouse CII and has homology to heat shock proteins.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
V. Taneja, N. Taneja, M. Behrens, M. M. Griffiths, H. S. Luthra, and C. S. David
Requirement for CD28 May Not Be Absolute for Collagen-Induced Arthritis: Study with HLA-DQ8 Transgenic Mice
J. Immunol., January 15, 2005; 174(2): 1118 - 1125.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. L. Greidinger, M. F. Foecking, J. Magee, L. Wilson, S. Ranatunga, R. A. Ortmann, and R. W. Hoffman
A Major B Cell Epitope Present on the Apoptotic but Not the Intact Form of the U1-70-kDa Ribonucleoprotein Autoantigen
J. Immunol., January 1, 2004; 172(1): 709 - 716.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Taneja, N. Taneja, M. Behrens, S. Pan, T. Trejo, M. Griffiths, H. Luthra, and C. S. David
HLA-DRB1*0402 (DW10) Transgene Protects Collagen- Induced Arthritis-Susceptible H2Aq and DRB1*0401 (DW4) Transgenic Mice from Arthritis
J. Immunol., October 15, 2003; 171(8): 4431 - 4438.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. J. Krco, S. Watanabe, J. Harders, M. M. Griffths, H. Luthra, and C. S. David
Identification of T Cell Determinants on Human Type II Collagen Recognized by HLA-DQ8 and HLA-DQ6 Transgenic Mice
J. Immunol., August 1, 1999; 163(3): 1661 - 1665.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.