The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nair, S. K.
Right arrow Articles by Gilboa, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nair, S. K.
Right arrow Articles by Gilboa, E.

The Journal of Immunology, Vol 156, Issue 5 1772-1780, Copyright © 1996 by American Association of Immunologists

ARTICLES

Cells treated with TAP-2 antisense oligonucleotides are potent antigen- presenting cells in vitro and in vivo

SK Nair, D Snyder and E Gilboa
Department of Surgery, Duke University Medical Center, Durham, NC 27710 USA.

Treatment of RMA and EL4 cells or freshly isolated splenocytes with antisense (AS) oligonucleotides directed against the TAP-2 gene recreates the phenotype seen in cells that are genetically deficient in TAP function. Cells incubated with AS oligonucleotides exhibit reduced MHC class I expression on the cell surface, which can be increased by incubating the oligonucleotide-treated cells at 28 degrees C or by adding MHC haplotype-matched peptides to the culture medium. RMA cells or splenocytes treated with AS oligonucleotides and incubated with peptide were highly effective in generating primary CTL responses in vitro. The bulk of the AS oligonucleotide-responsive and CTL-inducing cells resided in the adherent fraction of splenocytes. Moreover, TAP-2 AS oligonucleotide-treated adherent splenocytes pulsed with OVA peptide elicited potent OVA-specific CTL responses in vivo and provided effective protection from challenge with tumor cells expressing the corresponding Ag. AS oligonucleotide technology provides a simple approach to develop broadly applicable methods for generating potent APC to study TAP function in normal cells and to identify other gene products involved in MHC class I presentation.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
S. Manickasingham and C. Reis e Sousa
Microbial and T Cell-Derived Stimuli Regulate Antigen Presentation by Dendritic Cells In Vivo
J. Immunol., November 1, 2000; 165(9): 5027 - 5034.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. TANG, J. M. ROLLAND, X. LI, C. WARD, R. BISH, and E. HAYDN WALTERS
Alveolar Macrophages from Atopic Asthmatics, But Not Atopic Nonasthmatics, Enhance Interleukin-5 Production by CD4+ T Cells
Am. J. Respir. Crit. Care Med., April 1, 1998; 157(4): 1120 - 1126.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Z. Wolpert, M. Petersson, B. J. Chambers, J. K. Sandberg, R. Kiessling, H.-G. Ljunggren, and K. Karre
Generation of CD8+ T cells specific for transporter associated with antigen processing deficient cells
PNAS, October 14, 1997; 94(21): 11496 - 11501.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.