| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 156, Issue 4 1408-1414, Copyright © 1996 by American Association of Immunologists
ARTICLES |
I Maric, PG Holt, MH Perdue and J Bienenstock
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
Many tissues are found to contain populations of cells with an unusual dendritic shape, high levels of surface expression of MHC class II (Ia) gene products, and strong accessory function for the stimulation of specific clones of quiescent T lymphocytes. Dendritic cells (DC) represent major population of "professional" APC in various lymphoid and nonlymphoid tissues, distinct from cells of the monocyte/macrophage lineage. Among the best characterized nonlymphoid dendritic cells are epidermal Langerhans cells, but it has been shown that interstitium and epithelium of other organs also contain irregularly shaped, strongly MHC class II positive cells. In recent years, DC have been localized to alveolar septa in the lung, as well as within and just beneath airway epithelium, comprising a tightly meshed network that is reminiscent of epidermal Langerhans cells. In the gastrointestinal tract, conventional immunohistochemical analysis of mucosal class II MHC (Ia) staining reveals a morphologically heterogeneous pattern of staining in the lamina propria. DC that exhibit strong Ag-presenting activity in vitro have been extracted from enzymatic digests of colonic mucosa, but no previous reports of MHC class II-positive cells with pleiomorphic morphology have been recorded within the epithelium of the intestine. Employing a novel combination of nonconventional section planes, pre- embedding fixation, and immunohistochemical techniques, we now demonstrate Ia staining of cells with classical DC morphology within the epithelium of the intestine in normal specific pathogen-free rats. Our investigation suggests that cells with the morphologic and phenotypical characteristics of DC are present within the mucosal epithelium of the rat jejunum and colon, comprising a significant organized network. The number of DC within epithelium of the colon was 117 +/- 20 per 10-microns-thick cross-section. These findings have important theoretical implications for research on Ag processing and T cell activation in the context of allergic and infectious diseases in the gastrointestinal tract.
This article has been cited by other articles:
|
|
 |
|
  S. Hapfelmeier, A. J. Muller, B. Stecher, P. Kaiser, M. Barthel, K. Endt, M. Eberhard, R. Robbiani, C. A. Jacobi, M. Heikenwalder, et al. Microbe sampling by mucosal dendritic cells is a discrete, MyD88-independent stepin {Delta}invG S. Typhimurium colitis J. Exp. Med., February 18, 2008; 205(2): 437 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Flores-Langarica, S. Meza-Perez, J. Calderon-Amador, T. Estrada-Garcia, G. MacPherson, S. Lebecque, S. Saeland, R. M. Steinman, and L. Flores-Romo Network of dendritic cells within the muscular layer of the mouse intestine PNAS, December 27, 2005; 102(52): 19039 - 19044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Shanahan Physiological Basis for Novel Drug Therapies Used to Treat the Inflammatory Bowel Diseases I. Pathophysiological basis and prospects for probiotic therapy in inflammatory bowel disease Am J Physiol Gastrointest Liver Physiol, March 1, 2005; 288(3): G417 - G421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Niess, S. Brand, X. Gu, L. Landsman, S. Jung, B. A. McCormick, J. M. Vyas, M. Boes, H. L. Ploegh, J. G. Fox, et al. CX3CR1-Mediated Dendritic Cell Access to the Intestinal Lumen and Bacterial Clearance Science, January 14, 2005; 307(5707): 254 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Saurer, I. Seibold, S. Rihs, C. Vallan, T. Dumrese, and C. Mueller Virus-Induced Activation of Self-Specific TCR{alpha}{beta} CD8{alpha}{alpha} Intraepithelial Lymphocytes Does Not Abolish Their Self-Tolerance in the Intestine J. Immunol., April 1, 2004; 172(7): 4176 - 4183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nazli, P.-C. Yang, J. Jury, K. Howe, J. L. Watson, J. D. Soderholm, P. M. Sherman, M. H. Perdue, and D. M. McKay Epithelia Under Metabolic Stress Perceive Commensal Bacteria as a Threat Am. J. Pathol., March 1, 2004; 164(3): 947 - 957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. D. Smith, G. Meng, J. F. Salazar-Gonzalez, and G. M. Shaw Macrophage HIV-1 infection and the gastrointestinal tract reservoir J. Leukoc. Biol., November 1, 2003; 74(5): 642 - 649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A J Stagg, A L Hart, S C Knight, and M A Kamm The dendritic cell: its role in intestinal inflammation and relationship with gut bacteria Gut, October 1, 2003; 52(10): 1522 - 1529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kato, K. Fujihashi, R. Kato, T. Dohi, K. Fujihashi, Y. Hagiwara, K. Kataoka, R. Kobayashi, and J. R. McGhee Lack of oral tolerance in aging is due to sequential loss of Peyer's patch cell interactions Int. Immunol., February 1, 2003; 15(2): 145 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J E Collins Adhesion between dendritic cells and epithelial cells maintains the gut barrier during bacterial sampling Gut, April 1, 2002; 50(4): 449 - 450. [Full Text] [PDF]
|
|
|
|
 |
|
 F.-P. Huang, C. F. Farquhar, N. A. Mabbott, M. E. Bruce, and G. G. MacPherson Migrating intestinal dendritic cells transport PrPSc from the gut J. Gen. Virol., January 1, 2002; 83(1): 267 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Steinman and M. C. Nussenzweig Avoiding horror autotoxicus: The importance of dendritic cells in peripheral T cell tolerance PNAS, January 1, 2002; (2002) 231606698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Manohar, D. O. Baumann, N. A. Bos, and J. J. Cebra Gut Colonization of Mice with actA-Negative Mutant of Listeria monocytogenes Can Stimulate a Humoral Mucosal Immune Response Infect. Immun., June 1, 2001; 69(6): 3542 - 3549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Iwasaki and B. L. Kelsall Unique Functions of CD11b+, CD8{{alpha}}+, and Double-Negative Peyer's Patch Dendritic Cells J. Immunol., April 15, 2001; 166(8): 4884 - 4890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Bell, R. Rigby, N. English, S. D. Mann, S. C. Knight, M. A. Kamm, and A. J. Stagg Migration and Maturation of Human Colonic Dendritic Cells J. Immunol., April 15, 2001; 166(8): 4958 - 4967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Blanas, G. M. Davey, F. R. Carbone, and W. R. Heath A Bone Marrow-Derived APC in the Gut-Associated Lymphoid Tissue Captures Oral Antigens and Presents Them to Both CD4+ and CD8+ T Cells J. Immunol., March 15, 2000; 164(6): 2890 - 2896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Muller, M. Buhler-Jungo, and C. Mueller Intestinal Intraepithelial Lymphocytes Exert Potent Protective Cytotoxic Activity During an Acute Virus Infection J. Immunol., February 15, 2000; 164(4): 1986 - 1994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Shanahan Nutrient Tasting and Signaling Mechanisms in the Gut. V. Mechanisms of immunologic sensation of intestinal contents Am J Physiol Gastrointest Liver Physiol, February 1, 2000; 278(2): G191 - G196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Marguerat, H. R. MacDonald, J.-P. Kraehenbuhl, and J. P. M. van Meerwijk Protection from Radiation-Induced Colitis Requires MHC Class II Antigen Expression by Cells of Hemopoietic Origin J. Immunol., October 1, 1999; 163(7): 4033 - 4040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Perdue III. The mucosal antigen barrier: cross talk with mucosal cytokines Am J Physiol Gastrointest Liver Physiol, July 1, 1999; 277(1): G1 - G5. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. N. Lambrecht, I. Carro-Muino, K. Vermaelen, and R. A. Pauwels Allergen-Induced Changes in Bone-Marrow Progenitor and Airway Dendritic Cells in Sensitized Rats Am. J. Respir. Cell Mol. Biol., June 1, 1999; 20(6): 1165 - 1174. [Abstract] [Full Text]
|
|
|
|
|
|
A. Iwasaki and B. L. Kelsall I. Mucosal dendritic cells: their specialized role in initiating T cell responses Am J Physiol Gastrointest Liver Physiol, May 1, 1999; 276(5): G1074 - G1078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Viney, A. M. Mowat, J. M. O'Malley, E. Williamson, and N. A. Fanger Expanding Dendritic Cells In Vivo Enhances the Induction of Oral Tolerance J. Immunol., June 15, 1998; 160(12): 5815 - 5825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Steinhoff, U. Klemm, M. Greiner, K. Bordasch, and S. H. E. Kaufmann Altered Intestinal Immune System but Normal Antibacterial Resistance in the Absence of P-Selectin and ICAM-1 J. Immunol., June 15, 1998; 160(12): 6112 - 6120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Velin, G. Fotopoulos, F. Luthi, and J.-P. Kraehenbuhl The Nasal-associated Lymphoid Tissue of Adult Mice Acts as an Entry Site for the Mouse Mammary Tumor Retrovirus J. Exp. Med., May 19, 1997; 185(10): 1871 - 1876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Steinman and M. C. Nussenzweig Inaugural Article: Avoiding horror autotoxicus: The importance of dendritic cells in peripheral T cell tolerance PNAS, January 8, 2002; 99(1): 351 - 358. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
