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The Journal of Immunology, Vol 156, Issue 3 883-886, Copyright © 1996 by American Association of Immunologists

CUTTING EDGE

CD28-mediated up-regulation of beta 1-integrin adhesion involves phosphatidylinositol 3-kinase

T Zell, SW Hunt 3rd, JL Mobley, LD Finkelstein and Y Shimizu
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109, USA.

Cross-linking of the CD28 Ag on T cells results in increased beta 1- integrin-mediated adhesion to fibronectin. Chimeric contructs containing the CD28 cytoplasmic domain fused to the extracellular and transmembrane regions of CD2 were expressed in HL60 cells to investigate CD28-mediated regulation of adhesion. Ab cross-linking of the CD2/28 chimera resulted in increased beta 1-dependent adhesion of HL60 transfectants to fibronectin. Induced binding was completely inhibited by the phosphatidylinositol 3-kinase (PI 3-K) inhibitor wortmannin. Cross-linking of the CD2/28 chimera also induced association of the p85 subunit of PI 3-K with the CD2/28 cytoplasmic domain. In contrast, cross-linking of a CD2/28 chimera containing a tyrosine to phenylalanine substitution in the YMNM motif did not result in increased adhesion to fibronectin and did not lead to association of the chimera with PI 3-K. These results directly implicate the YMNM motif and PI 3-K in the regulation of beta 1-integrin activity by the CD28 Ag.


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