The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshimoto, T.
Right arrow Articles by Nariuchi, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshimoto, T.
Right arrow Articles by Nariuchi, H.

The Journal of Immunology, Vol 156, Issue 3 1082-1088, Copyright © 1996 by American Association of Immunologists

ARTICLES

Molecular cloning and characterization of murine IL-12 genes

T Yoshimoto, K Kojima, T Funakoshi, Y Endo, T Fujita and H Nariuchi
Department of Allergology, University of Tokyo, Japan.

IL-12 is a heterodimeric cytokine composed of two covalently linked chains, p40 and p35. p40 expression appears to be restricted to monocytes/macrophages and B cells and is highly regulated, while p35 is more ubiquitously and constitutively expressed. To investigate the mechanism involved in the regulation of IL-12 expression, we molecularly cloned and characterized the murine p40 and p35 genes. The p40 gene spans over 14 kb, consists of eight exons and seven introns, and was shown to be localized on chromosome 11A5-B2 by fluorescence in situ hybridization. A single major transcription initiation site was detected by primer extension analysis, and a TATA box was found at approximately 30 bp upstream from the transcription initiation site. The 5' flanking region preceding the transcription initiation site induced the enhanced expression of a promoterless reporter gene after LPS stimulation when transfected into a macrophage-like cell line. In contrast, the p35 gene spans over 8 kb and consists of seven exons and six introns on chromosome 6C, and multiple transcription initiation sites were detected. The 5' flanking region lacks canonical TATA and CAAT boxes at the appropriate position, but, instead, contains GC-rich sequences and constitutively mediated promoter activity when placed upstream of a promoterless reporter gene and transfected into a B cell lymphoma cell line. Thus, the characteristics of promoter regions of p40 and p35 genes are quite different, and this would account for the different regulations of p40 and p35 expression.


This article has been cited by other articles:


Home page
 Toxicol SciHome page
N. Ade, D. Antonios, S. Kerdine-Romer, F. Boisleve, F. Rousset, and M. Pallardy
NF-{kappa}B Plays a Major Role in the Maturation of Human Dendritic Cells Induced by NiSO4 but not by DNCB
Toxicol. Sci., October 1, 2007; 99(2): 488 - 501.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. L. Reinhardt, S. Hong, S.-J. Kang, Z.-e. Wang, and R. M. Locksley
Visualization of IL-12/23p40 In Vivo Reveals Immunostimulatory Dendritic Cell Migrants that Promote Th1 Differentiation
J. Immunol., August 1, 2006; 177(3): 1618 - 1627.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Kim, S. H. Kim, S. Kim, and T. S. Kim
The Novel Cytokine p43 Induces IL-12 Production in Macrophages via NF-{kappa}B Activation, Leading to Enhanced IFN-{gamma} Production in CD4+ T Cells
J. Immunol., January 1, 2006; 176(1): 256 - 264.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Y.-L. Lin, Y.-C. Liang, S.-S. Lee, and B.-L. Chiang
Polysaccharide purified from Ganoderma lucidum induced activation and maturation of human monocyte-derived dendritic cells by the NF-{kappa}B and p38 mitogen-activated protein kinase pathways
J. Leukoc. Biol., August 1, 2005; 78(2): 533 - 543.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
N. Schuetze, S. Schoeneberger, U. Mueller, M. A. Freudenberg, G. Alber, and R. K. Straubinger
IL-12 family members: differential kinetics of their TLR4-mediated induction by Salmonella Enteritidis and the impact of IL-10 in bone marrow-derived macrophages
Int. Immunol., May 1, 2005; 17(5): 649 - 659.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Chen, M. Zhao, R. Rao, H. Inoue, and C.-M. Hao
C/EBP{beta} and Its Binding Element Are Required for NF{kappa}B-induced COX2 Expression Following Hypertonic Stress
J. Biol. Chem., April 22, 2005; 280(16): 16354 - 16359.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. Hino, M.-N. Kweon, K. Fujihashi, J. R. McGhee, and H. Kiyono
Pathological Role of Large Intestinal IL-12p40 for the Induction of Th2-Type Allergic Diarrhea
Am. J. Pathol., April 1, 2004; 164(4): 1327 - 1335.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Cuesta, C. A. Salkowski, K. E. Thomas, and S. N. Vogel
Regulation of Lipopolysaccharide Sensitivity by IFN Regulatory Factor-2
J. Immunol., June 1, 2003; 170(11): 5739 - 5747.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
R. A. Jordan, M. T. Preissler, J. A. Banas, and E. J. Gosselin
Production of Genetically Engineered Biotinylated Interleukin-2 and Its Application in a Rapid Nonradioactive Assay for T-Cell Activation
Clin. Vaccine Immunol., May 1, 2003; 10(3): 339 - 344.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Maruyama, K. Sumita, H. Shen, M. Kanoh, X. Xu, M. Sato, M. Matsumoto, H. Shinomiya, and Y. Asano
Identification of IFN Regulatory Factor-1 Binding Site in IL-12 p40 Gene Promoter
J. Immunol., January 15, 2003; 170(2): 997 - 1001.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Kollet, C. Witek, J. D. Gentry, X. Liu, S. D. Schwartzbach, and T. M. Petro
Deletional Analysis of the Murine IL-12 p35 Promoter Comparing IFN-{gamma} and Lipopolysaccharide Stimulation
J. Immunol., November 15, 2001; 167(10): 5653 - 5663.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
R. Grumont, H. Hochrein, M. O'Keeffe, R. Gugasyan, C. White, I. Caminschi, W. Cook, and S. Gerondakis
c-Rel Regulates Interleukin 12 p70 Expression in CD8+ Dendritic Cells by Specifically Inducing p35 Gene Transcription
J. Exp. Med., October 8, 2001; 194(8): 1021 - 1032.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
O. Scharf, I. Agranovich, K. Lee, N. L. Eller, L. Levy, J. Inman, D. E. Scott, and B. Golding
Ontogeny of Th1 Memory Responses against a Brucella abortus Conjugate
Infect. Immun., September 1, 2001; 69(9): 5417 - 5422.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. R. TIMOSHANKO, A. R. KITCHING, S. R. HOLDSWORTH, and P. G. TIPPING
Interleukin-12 from Intrinsic Cells Is an Effector of Renal Injury in Crescentic Glomerulonephritis
J. Am. Soc. Nephrol., March 1, 2001; 12(3): 464 - 471.
[Abstract] [Full Text]

Home page
 Ann. N. Y. Acad. Sci.Home page
J. LECETA, R. P. GOMARIZ, C. MARTINEZ, C. ABAD, D. GANEA, and M. DELGADO
Receptors and Transcriptional Factors Involved in the Anti-Inflammatory Activity of VIP and PACAP
Ann. N.Y. Acad. Sci., December 1, 2000; 921(1): 92 - 102.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
I-M. Wang, C. Contursi, A. Masumi, X. Ma, G. Trinchieri, and K. Ozato
An IFN-{gamma}-Inducible Transcription Factor, IFN Consensus Sequence Binding Protein (ICSBP), Stimulates IL-12 p40 Expression in Macrophages
J. Immunol., July 1, 2000; 165(1): 271 - 279.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. L. Foss, M. J. Zilliox, and M. P. Murtaugh
Differential Regulation of Macrophage Interleukin-1 (IL-1), IL-12, and CD80-CD86 by Two Bacterial Toxins
Infect. Immun., October 1, 1999; 67(10): 5275 - 5281.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
C. Feng, S. Watanabe, S. Maruyama, G. Suzuki, M. Sato, T. Furuta, S. Kojima, S. Taki, and Y. Asano
An alternate pathway for type 1 T cell differentiation
Int. Immunol., August 1, 1999; 11(8): 1185 - 1194.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. A. Salkowski, K. Kopydlowski, J. Blanco, M. J. Cody, R. McNally, and S. N. Vogel
IL-12 Is Dysregulated in Macrophages from IRF-1 and IRF-2 Knockout Mice
J. Immunol., August 1, 1999; 163(3): 1529 - 1536.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Yamane, T. Kato, and H. Nariuchi
Effective Stimulation for IL-12 p35 mRNA Accumulation and Bioactive IL-12 Production of Antigen-Presenting Cells Interacted with Th Cells
J. Immunol., June 1, 1999; 162(11): 6433 - 6441.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Babik, E. Adams, Y. Tone, P. J. Fairchild, M. Tone, and H. Waldmann
Expression of Murine IL-12 Is Regulated by Translational Control of the p35 Subunit
J. Immunol., April 1, 1999; 162(7): 4069 - 4078.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-Y. Na, B. Y. Kang, S. W. Chung, S.-J. Han, X. Ma, G. Trinchieri, S.-Y. Im, J. W. Lee, and T. S. Kim
Retinoids Inhibit Interleukin-12 Production in Macrophages through Physical Associations of Retinoid X Receptor and NFkappa B
J. Biol. Chem., March 19, 1999; 274(12): 7674 - 7680.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Sam and M. M. Stevenson
In Vivo IL-12 Production and IL-12 Receptors {beta}1 and {beta}2 mRNA Expression in the Spleen Are Differentially Up-Regulated in Resistant B6 and Susceptible A/J Mice During Early Blood-Stage Plasmodium chabaudi AS Malaria
J. Immunol., February 1, 1999; 162(3): 1582 - 1589.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
M. DELGADO, E. J. MUNOZ-ELIAS, C. MARTINEZ, R. P. GOMARIZ, and D. GANEA
VIP and PACAP38 Modulate Cytokine and Nitric Oxide Production in Peritoneal Macrophages and Macrophage Cell Lines
Ann. N.Y. Acad. Sci., January 1, 1999; 897(1): 401 - 414.
[Full Text] [PDF]

Home page
 J. Exp. Med.Home page
P. A. Stumbles, J. A. Thomas, C. L. Pimm, P. T. Lee, T. J. Venaille, S. Proksch, and P. G. Holt
Resting Respiratory Tract Dendritic Cells Preferentially Stimulate T Helper Cell Type 2 (Th2) Responses and Require Obligatory Cytokine Signals for Induction of  Th1 Immunity
J. Exp. Med., December 7, 1998; 188(11): 2019 - 2031.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. P. Hayes, F. J. Murphy, and P. R. Burd
Interferon-gamma -Dependent Inducible Expression of the Human Interleukin-12 p35 Gene in Monocytes Initiates From a TATA-Containing Promoter Distinct From the CpG-Rich Promoter Active in Epstein-Barr Virus-Transformed Lymphoblastoid Cells
Blood, June 15, 1998; 91(12): 4645 - 4651.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. W. Chung, B. Y. Kang, S. H. Kim, Y. K. Pak, D. Cho, G. Trinchieri, and T. S. Kim
Oxidized Low Density Lipoprotein Inhibits Interleukin-12 Production in Lipopolysaccharide-activated Mouse Macrophages via Direct Interactions between Peroxisome Proliferator-activated Receptor-gamma and Nuclear Factor-kappa B
J. Biol. Chem., October 13, 2000; 275(42): 32681 - 32687.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.