| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 156, Issue 2 758-764, Copyright © 1996 by American Association of Immunologists
ARTICLES |
Y Onoe, C Miyaura, T Kaminakayashiki, Y Nagai, K Noguchi, QR Chen, H Seo, H Ohta, S Nozawa, I Kudo and T Suda
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
Activated T cells secrete the cytokine IL-13, which regulates inflammatory and immune responses. To explore the role of IL-13 in bone metabolism, we examined the effects of the cytokine on bone resorption and PG synthesis in osteoblasts. IL-13 suppressed the bone-resorbing activity stimulated by IL-1 alpha, which was determined by the release of 45Ca from prelabeled mouse long bones. Histologic examinations revealed that IL-1 alpha markedly stimulated bone resorption with increased osteoclast recruitment, and that the simultaneous addition of IL-13 considerably inhibited it. The gamma-chain of IL-2 receptors may be functionally involved in the signal transduction of not only IL-2, but also IL-4, IL-7, and IL-13. Of these cytokines, IL-4 similarly suppressed IL-1 alpha-induced bone resorption, but IL-2 and IL-7 did not. Both IL-13 and IL-4 inhibited PGE2 production stimulated by IL-1 alpha in long bone cultures. Suppression of IL-1 alpha-induced bone resorption by IL-13 and IL-4 was recovered by adding exogenous PGE2 to the long bone cultures. Neither IL-4 nor IL-13 further inhibited IL-1 alpha-induced bone resorption in the presence of indomethacin. To examine the effects of IL-13 on PG synthesis, we measured the mRNA levels of cytosolic phospholipase A2 (cPLA2), constitutively expressed cyclooxygenase (COX-1) and inducible COX (COX-2) in mouse osteoblast- like cells. IL-1 alpha markedly stimulated the mRNA expression of COX- 2, but not that of COX-1. Both IL-13 and IL-4 dose-dependently suppressed the IL-1 alpha-induced stimulation of both COX-2 mRNA expression and PGE2 synthesis. A small increase (1.7-fold) in cPLA2 mRNA levels was detected in the cultures with IL-1 alpha, but the expression was not affected by IL-13 or IL-4. These results indicated that IL-13 and IL-4 inhibit bone resorption by suppressing COX-2- dependent PG synthesis in osteoblasts.
This article has been cited by other articles:
|
|
 |
|
  J. Lorenzo, M. Horowitz, and Y. Choi Osteoimmunology: Interactions of the Bone and Immune System Endocr. Rev., June 1, 2008; 29(4): 403 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.A. Silva, G.P. Garlet, S.Y. Fukada, J.S. Silva, and F.Q. Cunha Chemokines in Oral Inflammatory Diseases: Apical Periodontitis and Periodontal Disease J. Dent. Res., April 1, 2007; 86(4): 306 - 319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.A. Silva, G.P. Garlet, S.Y. Fukada, J.S. Silva, and F.Q. Cunha Chemokines in Oral Inflammatory Diseases: Apical Periodontitis and Periodontal Disease Journal of Dental Research, April 1, 2007; 86(4): 306 - 319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Palmqvist, P. Lundberg, E. Persson, A. Johansson, I. Lundgren, A. Lie, H. H. Conaway, and U. H. Lerner Inhibition of Hormone and Cytokine-stimulated Osteoclastogenesis and Bone Resorption by Interleukin-4 and Interleukin-13 Is Associated with Increased Osteoprotegerin and Decreased RANKL and RANK in a STAT6-dependent Pathway J. Biol. Chem., February 3, 2006; 281(5): 2414 - 2429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Young, N. Mikhalkevich, Y. Yan, D. Chen, and W.-p. Zheng Differential Regulation of Osteoblast Activity by Th Cell Subsets Mediated by Parathyroid Hormone and IFN-{gamma} J. Immunol., December 15, 2005; 175(12): 8287 - 8295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Okahashi, H. Inaba, I. Nakagawa, T. Yamamura, M. Kuboniwa, K. Nakayama, S. Hamada, and A. Amano Porphyromonas gingivalis Induces Receptor Activator of NF-{kappa}B Ligand Expression in Osteoblasts through the Activator Protein 1 Pathway Infect. Immun., March 1, 2004; 72(3): 1706 - 1714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-K. Lee, J. F. Kalinowski, S. L. Jastrzebski, L. Puddington, and J. A. Lorenzo Interleukin-7 Is a Direct Inhibitor of in Vitro Osteoclastogenesis Endocrinology, August 1, 2003; 144(8): 3524 - 3531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Okahashi, A. Sakurai, I. Nakagawa, T. Fujiwara, S. Kawabata, A. Amano, and S. Hamada Infection by Streptococcus pyogenes Induces the Receptor Activator of NF-{kappa}B Ligand Expression in Mouse Osteoblastic Cells Infect. Immun., February 1, 2003; 71(2): 948 - 955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Vale, J. B. Marques, C. A. Moreira, F. A. C. Rocha, S. H. Ferreira, S. Poole, F. Q. Cunha, and R. A. Ribeiro Antinociceptive Effects of Interleukin-4, -10, and -13 on the Writhing Response in Mice and Zymosan-Induced Knee Joint Incapacitation in Rats J. Pharmacol. Exp. Ther., January 1, 2003; 304(1): 102 - 108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Bouchard, R. de Medicis, A. Lussier, P. H. Naccache, and P. E. Poubelle Inflammatory Microcrystals Alter the Functional Phenotype of Human Osteoblast-Like Cells In Vitro: Synergism with IL-1 to Overexpress Cyclooxygenase-2 J. Immunol., May 15, 2002; 168(10): 5310 - 5317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Hinz and K. Brune Cyclooxygenase-2---10 Years Later J. Pharmacol. Exp. Ther., February 1, 2002; 300(2): 367 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C S Cleaver, A D Rowan, and T E Cawston Interleukin 13 blocks the release of collagen from bovine nasal cartilage treated with proinflammatory cytokines Ann Rheum Dis, February 1, 2001; 60(2): 150 - 157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.A. Taubman and T. Kawai Involvement of T-Lymphocytes in Periodontal Disease and in Direct and Indirect Induction of Bone Resorption Critical Reviews in Oral Biology & Medicine, January 1, 2001; 12(2): 125 - 135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sasaki, L. Hou, A. Belani, C.-Y. Wang, T. Uchiyama, R. Muller, and P. Stashenko IL-10, But Not IL-4, Suppresses Infection-Stimulated Bone Resorption In Vivo J. Immunol., October 1, 2000; 165(7): 3626 - 3630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Woods, K. J. Katschke Jr., M. Tokuhira, H. Kurata, K.-I. Arai, P. L. Campbell, and A. E. Koch Reduction of Inflammatory Cytokines and Prostaglandin E2 by IL-13 Gene Therapy in Rheumatoid Arthritis Synovium J. Immunol., September 1, 2000; 165(5): 2755 - 2763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Lipsky, P. Brooks, L. J. Crofford, R. DuBois, D. Graham, L. S. Simon, L. B. A. van de Putte, and S. B. Abramson Unresolved Issues in the Role of Cyclooxygenase-2 in Normal Physiologic Processes and Disease Arch Intern Med, April 10, 2000; 160(7): 913 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Suzawa, C. Miyaura, M. Inada, T. Maruyama, Y. Sugimoto, F. Ushikubi, A. Ichikawa, S. Narumiya, and T. Suda The Role of Prostaglandin E Receptor Subtypes (EP1, EP2, EP3, and EP4) in Bone Resorption: An Analysis Using Specific Agonists for the Respective EPs Endocrinology, April 1, 2000; 141(4): 1554 - 1559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kawai, R. Eisen-Lev, M. Seki, J. W. Eastcott, M. E. Wilson, and M. A. Taubman Requirement of B7 Costimulation for Th1-Mediated Inflammatory Bone Resorption in Experimental Periodontal Disease J. Immunol., February 15, 2000; 164(4): 2102 - 2109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. G Roberge, M. D de Smet, J. Benichou, M. F Kriete, J. Raber, and J. Hakimi Treatment of uveitis with recombinant human interleukin-13 Br. J. Ophthalmol., October 1, 1998; 82(10): 1195 - 1198. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Frost, K. B. Jonsson, H. Brändström, C. Ohlsson, S. Ljunghall, and O. Ljunggren Interleukin-13 Inhibits Cell Proliferation and Stimulates Interleukin-6 Formation in Isolated Human Osteoblasts J. Clin. Endocrinol. Metab., September 1, 1998; 83(9): 3285 - 3289. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. N. Dubois, S. B. Abramson, L. Crofford, R. A. Gupta, L. S. Simon, L. B. A. van de putte, and P. E. Lipsky Cyclooxygenase in biology and disease FASEB J, September 1, 1998; 12(12): 1063 - 1073. [Abstract] [Full Text]
|
|
|
|
|
|
K. Kusano, C. Miyaura, M. Inada, T. Tamura, A. Ito, H. Nagase, K. Kamoi, and T. Suda Regulation of Matrix Metalloproteinases (MMP-2, -3, -9, and -13) by Interleukin-1 and Interleukin-6 in Mouse Calvaria: Association of MMP Induction with Bone Resorption Endocrinology, March 1, 1998; 139(3): 1338 - 1345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Miyaura, Y. Onoe, M. Inada, K. Maki, K. Ikuta, M. Ito, and T. Suda Increased B-lymphopoiesis by interleukin 7 induces bone loss in mice with intact ovarian function: Similarity to estrogen deficiency PNAS, August 19, 1997; 94(17): 9360 - 9365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tai, C. Miyaura, C. C. Pilbeam, T. Tamura, Y. Ohsugi, Y. Koishihara, N. Kubodera, H. Kawaguchi, L. G. Raisz, and T. Suda Transcriptional Induction of Cyclooxygenase-2 in Osteoblasts Is Involved in Interleukin-6-Induced Osteoclast Formation Endocrinology, June 1, 1997; 138(6): 2372 - 2379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q.-R. Chen, C. Miyaura, S. Higashi, M. Murakami, I. Kudo, S. Saito, T. Hiraide, Y. Shibasaki, and T. Suda Activation of Cytosolic Phospholipase A2 by Platelet-derived Growth Factor Is Essential for Cyclooxygenase-2-dependent Prostaglandin E2 Synthesis in Mouse Osteoblasts Cultured with Interleukin-1 J. Biol. Chem., February 28, 1997; 272(9): 5952 - 5958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Miyaura, M. Inada, T. Suzawa, Y. Sugimoto, F. Ushikubi, A. Ichikawa, S. Narumiya, and T. Suda Impaired Bone Resorption to Prostaglandin E2 in Prostaglandin E Receptor EP4-knockout Mice J. Biol. Chem., June 23, 2000; 275(26): 19819 - 19823. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
