The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gomes, N. A.
Right arrow Articles by DosReis, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gomes, N. A.
Right arrow Articles by DosReis, G. A.

The Journal of Immunology, Vol 156, Issue 2 628-635, Copyright © 1996 by American Association of Immunologists

ARTICLES

Down-regulation of T lymphocyte activation in vitro and in vivo induced by glycoinositolphospholipids from Trypanosoma cruzi. Assignment of the T cell-suppressive determinant to the ceramide domain

NA Gomes, JO Previato, B Zingales, L Mendonca-Previato and GA DosReis
Institute of Microbiology, Federal University of Rio de Janeiro, Brazil.

The major surface glycoinositolphospholipid (GIPL) from Trypanosoma cruzi was purified and assessed in mouse T cell function assays. Purified GIPLs from T. cruzi strains Y and G, but not from a plant trypanosomatid (Phytomonas serpens), markedly blocked in vitro CD4+ and CD8+ T cell mitogenesis induced by bacterial superantigen and anti- TCR;CD3 Abs. Secretion of IL-2, but not of IL-4, bioactivity, was reduced by GIPLs. T. cruzi, but not P. serpens, GIPL also blocked recall cellular responses to T. cruiz. GIPLs from T. cruzi, but not from P. serpens, blocked in vivo regional lymph node T cell activation induced by anti-CD3 mAb. Blockage led to loss of IL-2 responsiveness, with inhibition of CD25 expression on both CD4+ and CD8+ subsets. Isolated phosphoinositol oligosaccharides from GIPLs had no effect on in vitro CD4+ T cell mitogenesis. Isolated ceramide from T. cruzi GIPLs contained mainly N-lignoceroyldihydrosphingosine and blocked CD4+ T cell activation in vitro with the same potency as the intact GIPL. Standard N-palmitoylsphingosine, but not N-palmitoyldihydrosphingosine, blocked CD4+ T cell mitogenesis. A longer fatty acid chain, such as in standard N-lignoceroyldihydrosphingosine, or in the natural trypanosomal GIPL-derived ceramide, however, conferred full inhibitory effects on CD4+ T cells. These results demonstrate that T. cruzi GIPL has T cell immunomodulatory activity in vitro and in vivo, and that this novel activity maps to the ceramide domain. These findings could have implications for immunologic disturbances induced in the host by the causative agent of Chagas' disease.


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
M. Chamekh, V. Vercruysse, M. Habib, M. Lorent, M. Goldman, A. Allaoui, and B. Vray
Transfection of Trypanosoma cruzi with Host CD40 Ligand Results in Improved Control of Parasite Infection
Infect. Immun., October 1, 2005; 73(10): 6552 - 6561.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A.-C. Oliveira, J. R. Peixoto, L. B. de Arruda, M. A. Campos, R. T. Gazzinelli, D. T. Golenbock, S. Akira, J. O. Previato, L. Mendonca-Previato, A. Nobrega, et al.
Expression of Functional TLR4 Confers Proinflammatory Responsiveness to Trypanosoma cruzi Glycoinositolphospholipids and Higher Resistance to Infection with T. cruzi
J. Immunol., November 1, 2004; 173(9): 5688 - 5696.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
P. Alcaide and M. Fresno
The Trypanosoma cruzi membrane mucin AgC10 inhibits T cell activation and IL-2 transcription through L-selectin
Int. Immunol., October 1, 2004; 16(10): 1365 - 1375.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Abboushi, A. El-Hed, W. El-Assaad, L. Kozhaya, M. E. El-Sabban, A. Bazarbachi, R. Badreddine, A. Bielawska, J. Usta, and G. S. Dbaibo
Ceramide Inhibits IL-2 Production by Preventing Protein Kinase C-Dependent NF-{kappa}B Activation: Possible Role in Protein Kinase C{theta} Regulation
J. Immunol., September 1, 2004; 173(5): 3193 - 3200.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. A. Campos, M. Closel, E. P. Valente, J. E. Cardoso, S. Akira, J. I. Alvarez-Leite, C. Ropert, and R. T. Gazzinelli
Impaired Production of Proinflammatory Cytokines and Host Resistance to Acute Infection with Trypanosoma cruzi in Mice Lacking Functional Myeloid Differentiation Factor 88
J. Immunol., February 1, 2004; 172(3): 1711 - 1718.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. Brodskyn, J. Patricio, R. Oliveira, L. Lobo, A. Arnholdt, L. Mendonca-Previato, A. Barral, and M. Barral-Netto
Glycoinositolphospholipids from Trypanosoma cruzi Interfere with Macrophages and Dendritic Cell Responses
Infect. Immun., July 1, 2002; 70(7): 3736 - 3743.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. A. S. Campos, I. C. Almeida, O. Takeuchi, S. Akira, E. P. Valente, D. O. Procopio, L. R. Travassos, J. A. Smith, D. T. Golenbock, and R. T. Gazzinelli
Activation of Toll-Like Receptor-2 by Glycosylphosphatidylinositol Anchors from a Protozoan Parasite
J. Immunol., July 1, 2001; 167(1): 416 - 423.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. B. de Arruda Hinds, L. M. Previato, J. O. Previato, Q. Vos, J. J. Mond, and L. M. T. Pecanha
Modulation of B-Lymphocyte and NK Cell Activities by Glycoinositolphospholipid Purified from Trypanosoma cruzi
Infect. Immun., November 1, 1999; 67(11): 6177 - 6180.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. BELLIO, A.-C. S. C. O LIVEIRA, C. S. MERMELSTEIN, M. A. M. CAPELLA, J. P. B. VIOLA, J.-P. LEVRAUD, G. A. DOSREIS, J. O. PREVIATO, and L. MENDONÇA-PREVIATO
Costimulatory action of glycoinositolphospholipids from Trypanosoma cruzi: increased interleukin 2 secretion and induction of nuclear translocation of the nuclear factor of activated T cells 1
FASEB J, September 1, 1999; 13(12): 1627 - 1636.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
L. Van Overtvelt, N. Vanderheyde, V. Verhasselt, J. Ismaili, L. De Vos, M. Goldman, F. Willems, and B. Vray
Trypanosoma cruzi Infects Human Dendritic Cells and Prevents Their Maturation: Inhibition of Cytokines, HLA-DR, And Costimulatory Molecules
Infect. Immun., August 1, 1999; 67(8): 4033 - 4040.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. G. Freire-de-Lima, M. P. Nunes, S. Corte-Real, M. P. Soares, J. O. Previato, L. Mendonca-Previato, and G. A. DosReis
Proapoptotic Activity of a Trypanosoma cruzi Ceramide-Containing Glycolipid Turned on in Host Macrophages by IFN-{gamma}
J. Immunol., November 1, 1998; 161(9): 4909 - 4916.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Lochnit, R. D. Dennis, A. J. Ulmer, and R. Geyer
Structural Elucidation and Monokine-inducing Activity of Two Biologically Active Zwitterionic Glycosphingolipids Derived from the Porcine Parasitic Nematode Ascaris suum
J. Biol. Chem., January 2, 1998; 273(1): 466 - 474.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Galley, G. Hagens, I. Glaser, W. Davis, M. Eichhorn, and D. Dobbelaere
Jun NH2-terminal kinase is constitutively activated in T cells transformed by the intracellular parasite Theileria parva
PNAS, May 13, 1997; 94(10): 5119 - 5124.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.