The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yi, A. K.
Right arrow Articles by Krieg, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yi, A. K.
Right arrow Articles by Krieg, A. M.

The Journal of Immunology, Vol 156, Issue 2 558-564, Copyright © 1996 by American Association of Immunologists

ARTICLES

IFN-gamma promotes IL-6 and IgM secretion in response to CpG motifs in bacterial DNA and oligodeoxynucleotides

AK Yi, JH Chace, JS Cowdery and AM Krieg
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USA.

Lymphocyte recognition of characteristic structural features in microbial DNA may contribute to immune defense by promoting protective immune responses. The dinucleotide CpG is significantly under- represented in vertebrate DNA and is usually methylated. In contrast, CpG dinucleotides are generally present at the expected frequency in bacterial DNA and are unmethylated. These unmethylated CpG motifs induce B cells to secrete IL-6 and IgM, and can induce NK and CD4+ T cells to produce the immunoregulatory Th1 cytokine, IFN-gamma. IFN- gamma inhibits IgM secretion that is triggered by a different bacterial product, LPS. The present study demonstrates that in contrast to its antagonistic interaction with LPS, IFN-gamma causes a dose-dependent increase in the level of IgM secretion induced by CpG DNA. Like IgM secretion, B cell secretion of IL-6 more than doubles after the addition of exogenous IFN-gamma. Mice with disrupted IFN-gamma genes produced less than half as much IL-6 and IgM in response to CpG DNA, supporting the hypothesis that CpG-induced IFN-gamma production contributes to the B cell response. In contrast to its promotion of IL- 6 and IgM secretion, IFN-gamma did not significantly affect the spleen cell proliferation activated by CpG motifs. These results indicate that IFN-gamma produced by T and NK cells after CpG DNA stimulation contributes to the B cell production of IL-6 and the subsequent Ig production. These studies provide further evidence that the immune system responds to CpG motifs in bacterial DNA by activating a coordinated set of humoral and cellular responses.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
J.-E. Park, Y.-I. Kim, and A.-K. Yi
Protein Kinase D1: A New Component in TLR9 Signaling
J. Immunol., August 1, 2008; 181(3): 2044 - 2055.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. T. Collins, J. Shi, B. E. Burrell, D. K. Bishop, and W. A. Dunnick
Induced Expression of Murine {gamma}2a by CD40 Ligation Independently of IFN-{gamma}
J. Immunol., October 15, 2006; 177(8): 5414 - 5419.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. H. Harris, N. M. Cooney, J. M. Mansfield, and D. M. Paulnock
Signal transduction, gene transcription, and cytokine production triggered in macrophages by exposure to trypanosome DNA.
Infect. Immun., August 1, 2006; 74(8): 4530 - 4537.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A.-K. Yi, H. Yoon, J.-E. Park, B.-S. Kim, H. J. Kim, and A. Martinez-Hernandez
CpG DNA-mediated Induction of Acute Liver Injury in D-Galactosamine-sensitized Mice: THE MITOCHONDRIAL APOPTOTIC PATHWAY-DEPENDENT DEATH OF HEPATOCYTES
J. Biol. Chem., May 26, 2006; 281(21): 15001 - 15012.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
W. Kozak, S. Wrotek, and A. Kozak
Pyrogenicity of CpG-DNA in mice: role of interleukin-6, cyclooxygenases, and nuclear factor-{kappa}B
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R871 - R880.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. P. Ho, P. Fontoura, M. Platten, R. A. Sobel, J. J. DeVoss, L. Y. Lee, B. A. Kidd, B. H. Tomooka, J. Capers, A. Agrawal, et al.
A Suppressive Oligodeoxynucleotide Enhances the Efficacy of Myelin Cocktail/IL-4-Tolerizing DNA Vaccination and Treats Autoimmune Disease
J. Immunol., November 1, 2005; 175(9): 6226 - 6234.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. L. Fields, S. A. Nish, B. D. Hondowicz, M. H. Metzgar, G. N. Wharton, A. J. Caton, and J. Erikson
The Influence of Effector T Cells and Fas Ligand on Lupus-Associated B Cells
J. Immunol., July 1, 2005; 175(1): 104 - 111.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. L. McCoy, S. E. Kurtz, F. A. Hausman, D. R. Trune, R. M. Bennett, and S. H. Hefeneider
Activation of RAW264.7 Macrophages by Bacterial DNA and Lipopolysaccharide Increases Cell Surface DNA Binding and Internalization
J. Biol. Chem., April 23, 2004; 279(17): 17217 - 17223.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Storni, C. Ruedl, K. Schwarz, R. A. Schwendener, W. A. Renner, and M. F. Bachmann
Nonmethylated CG Motifs Packaged into Virus-Like Particles Induce Protective Cytotoxic T Cell Responses in the Absence of Systemic Side Effects
J. Immunol., February 1, 2004; 172(3): 1777 - 1785.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. B. Jorgensen, L.-H. Johansen, K. Steiro, and A. Johansen
CpG DNA Induces Protective Antiviral Immune Responses in Atlantic Salmon (Salmo salar L.)
J. Virol., November 1, 2003; 77(21): 11471 - 11479.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-J. Yeo, J.-G. Yoon, and A.-K. Yi
Myeloid Differentiation Factor 88-dependent Post-transcriptional Regulation of Cyclooxygenase-2 Expression by CpG DNA: TUMOR NECROSIS FACTOR-{alpha} RECEPTOR-ASSOCIATED FACTOR 6, A DIVERGING POINT IN THE Toll-LIKE RECEPTOR 9-SIGNALING
J. Biol. Chem., October 17, 2003; 278(42): 40590 - 40600.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. J. Kemp, B. D. Elzey, and T. S. Griffith
Plasmacytoid Dendritic Cell-Derived IFN-{alpha} Induces TNF-Related Apoptosis-Inducing Ligand/Apo-2L-Mediated Antitumor Activity by Human Monocytes Following CpG Oligodeoxynucleotide Stimulation
J. Immunol., July 1, 2003; 171(1): 212 - 218.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
A.-K. Yi, J.-G. Yoon, and A. M. Krieg
Convergence of CpG DNA- and BCR-mediated signals at the c-Jun N-terminal kinase and NF-{kappa}B activation pathways: regulation by mitogen-activated protein kinases
Int. Immunol., May 1, 2003; 15(5): 577 - 591.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S.-J. Yeo, J.-G. Yoon, S.-C. Hong, and A.-K. Yi
CpG DNA Induces Self and Cross-Hyporesponsiveness of RAW264.7 Cells in Response to CpG DNA and Lipopolysaccharide: Alterations in IL-1 Receptor-Associated Kinase Expression
J. Immunol., January 15, 2003; 170(2): 1052 - 1061.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. K. Choudhury, J. S. Wild, R. Alam, D. M. Klinman, I. Boldogh, N. Dharajiya, W. J. Mileski, and S. Sur
In Vivo Role of p38 Mitogen-Activated Protein Kinase in Mediating the Anti-inflammatory Effects of CpG Oligodeoxynucleotide in Murine Asthma
J. Immunol., November 15, 2002; 169(10): 5955 - 5961.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A.-K. Yi, J.-G. Yoon, S.-J. Yeo, S.-C. Hong, B. K. English, and A. M. Krieg
Role of Mitogen-Activated Protein Kinases in CpG DNA-Mediated IL-10 and IL-12 Production: Central Role of Extracellular Signal-Regulated Kinase in the Negative Feedback Loop of the CpG DNA-Mediated Th1 Response
J. Immunol., May 1, 2002; 168(9): 4711 - 4720.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
A.-K. Yi, J.-G. Yoon, S.-C. Hong, T. W. Redford, and A. M. Krieg
Lipopolysaccharide and CpG DNA synergize for tumor necrosis factor-{alpha} production through activation of NF-{kappa}B
Int. Immunol., November 1, 2001; 13(11): 1391 - 1404.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. R. Bosarge, J. M. Watt, D. O. McDaniel, E. Swiatlo, and L. S. McDaniel
Genetic Immunization with the Region Encoding the {alpha}-Helical Domain of PspA Elicits Protective Immunity against Streptococcus pneumoniae
Infect. Immun., September 1, 2001; 69(9): 5456 - 5463.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
Y. Chen, J. Zhang, S. A. Moore, Z. K. Ballas, J. P. Portanova, A. M. Krieg, and D. J. Berg
CpG DNA induces cyclooxygenase-2 expression and prostaglandin production
Int. Immunol., August 1, 2001; 13(8): 1013 - 1020.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
E. Malanchere-Bres, P. J. Payette, M. Mancini, P. Tiollais, H. L. Davis, and M.-L. Michel
CpG Oligodeoxynucleotides with Hepatitis B Surface Antigen (HBsAg) for Vaccination in HBsAg-Transgenic Mice
J. Virol., July 15, 2001; 75(14): 6482 - 6491.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
M. Hafner, R. Zawatzky, C. Hirtreiter, W. A. Buurman, B. Echtenacher, T. Hehlgans, and D. N. Mannel
Antimetastatic Effect of CpG DNA Mediated by Type I IFN
Cancer Res., July 1, 2001; 61(14): 5523 - 5528.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. D. Crabtree, L. Jin, D. P. Raymond, S. J. Pelletier, C. W. Houlgrave, T. G. Gleason, T. L. Pruett, and R. G. Sawyer
Preexposure of Murine Macrophages to CpG Oligonucleotide Results in a Biphasic Tumor Necrosis Factor Alpha Response to Subsequent Lipopolysaccharide Challenge
Infect. Immun., April 1, 2001; 69(4): 2123 - 2129.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
G. J. Weiner
The immunobiology and clinical potential of immunostimulatory CpG oligodeoxynucleotides
J. Leukoc. Biol., October 1, 2000; 68(4): 455 - 463.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
M. G. Chiaramonte, M. Hesse, A. W. Cheever, and T. A. Wynn
CpG Oligonucleotides Can Prophylactically Immunize Against Th2-Mediated Schistosome Egg-Induced Pathology by an IL-12-Independent Mechanism
J. Immunol., January 15, 2000; 164(2): 973 - 985.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. P. Sester, S. J. Beasley, M. J. Sweet, L. F. Fowles, S. L. Cronau, K. J. Stacey, and D. A. Hume
Bacterial/CpG DNA Down-Modulates Colony Stimulating Factor-1 Receptor Surface Expression on Murine Bone Marrow-Derived Macrophages with Concomitant Growth Arrest and Factor-Independent Survival
J. Immunol., December 15, 1999; 163(12): 6541 - 6550.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
A.-K. Yi, D. W. Peckham, R. F. Ashman, and A. M. Krieg
CpG DNA rescues B cells from apoptosis by activating NF{kappa}B and preventing mitochondrial membrane potential disruption via a chloroquine-sensitive pathway
Int. Immunol., December 1, 1999; 11(12): 2015 - 2024.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
S.-S. Han, S.-T. Chung, D. A. Robertson, R. L. Chelvarajan, and S. Bondada
CpG oligodeoxynucleotides rescue BKS-2 immature B cell lymphoma from anti-IgM-mediated growth inhibition by up-regulation of egr-1
Int. Immunol., June 1, 1999; 11(6): 871 - 879.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Li, S.-P. Wu, M. Whitmore, E. J. Loeffert, L. Wang, S. C. Watkins, B. R. Pitt, and L. Huang
Effect of immune response on gene transfer to the lung via systemic administration of cationic lipidic vectors
Am J Physiol Lung Cell Mol Physiol, May 1, 1999; 276(5): L796 - L804.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H.-M. Liu, S. E. Newbrough, S. K. Bhatia, C. E. Dahle, A. M. Krieg, and G. J. Weiner
Immunostimulatory CpG Oligodeoxynucleotides Enhance the Immune Response to Vaccine Strategies Involving Granulocyte-Macrophage Colony-Stimulating Factor
Blood, November 15, 1998; 92(10): 3730 - 3736.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A.-K. Yi and A. M. Krieg
Cutting Edge: Rapid Induction of Mitogen-Activated Protein Kinases by Immune Stimulatory CpG DNA
J. Immunol., November 1, 1998; 161(9): 4493 - 4497.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. M. Krieg, T. Wu, R. Weeratna, S. M. Efler, L. Love-Homan, L. Yang, A.-K. Yi, D. Short, and H. L. Davis
Sequence motifs in adenoviral DNA block immune activation by stimulatory CpG motifs
PNAS, October 13, 1998; 95(21): 12631 - 12636.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. M. Krieg, L. Love-Homan, A.-K. Yi, and J. T. Harty
CpG DNA Induces Sustained IL-12 Expression In Vivo and Resistance to Listeria monocytogenes Challenge
J. Immunol., September 1, 1998; 161(5): 2428 - 2434.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A.-K. Yi, M. Chang, D. W. Peckham, A. M. Krieg, and R. F. Ashman
CpG Oligodeoxyribonucleotides Rescue Mature Spleen B Cells from Spontaneous Apoptosis and Promote Cell Cycle Entry
J. Immunol., June 15, 1998; 160(12): 5898 - 5906.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A.-K. Yi and A. M. Krieg
CpG DNA Rescue from Anti-IgM-Induced WEHI-231 B Lymphoma Apoptosis via Modulation of I{kappa}B{alpha} and I{kappa}B{beta} and Sustained Activation of Nuclear Factor-{kappa}B/c-Rel
J. Immunol., February 1, 1998; 160(3): 1240 - 1245.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. L. Davis, R. Weeranta, T. J. Waldschmidt, L. Tygrett, J. Schorr, and A. M. Krieg
CpG DNA Is a Potent Enhancer of Specific Immunity in Mice Immunized with Recombinant Hepatitis B Surface Antigen
J. Immunol., January 15, 1998; 160(2): 870 - 876.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.