| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 156, Issue 12 4594-4601, Copyright © 1996 by American Association of Immunologists
ARTICLES |
LP Kane and SM Hedrick
Department of Biology, University of California-San Diego, La Jolla, CA 92093, USA.
We have applied an in vitro system that mimics thymic negative selection to investigate signaling pathways that may be important for the removal of autoreactive cells from the thymus. We sought to more precisely determine the contribution of calcium-dependent pathways to CD4+CD8+ thymocyte deletion that is mediated by either an antigenic peptide or a peptide analogue. We show that the requirement for external calcium influx is dependent upon the strength of the deleting ligand. Furthermore, these results correlate well with a requirement, under certain circumstances, for signaling through the calcium/calmodulin-dependent phosphatase calcineurin. The use of suboptimal stimuli may, therefore, be useful in revealing biochemical pathways important for CD4+CD8+ thymocyte negative selection.
This article has been cited by other articles:
|
|
 |
|
  A. K. Patra, T. Drewes, S. Engelmann, S. Chuvpilo, H. Kishi, T. Hunig, E. Serfling, and U. H. Bommhardt PKB Rescues Calcineurin/NFAT-Induced Arrest of Rag Expression and Pre-T Cell Differentiation J. Immunol., October 1, 2006; 177(7): 4567 - 4576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Cante-Barrett, E. M. Gallo, M. M. Winslow, and G. R. Crabtree Thymocyte Negative Selection Is Mediated by Protein Kinase C- and Ca2+-Dependent Transcriptional Induction of Bim J. Immunol., February 15, 2006; 176(4): 2299 - 2306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Koonpaew, S. Shen, L. Flowers, and W. Zhang LAT-mediated signaling in CD4+CD25+ regulatory T cell development J. Exp. Med., January 23, 2006; 203(1): 119 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Friedrich, B. G. Wen, G. Bain, B. L. Kee, C. Katayama, C. Murre, S. M. Hedrick, and C. M. Walsh DRAK2, a lymphoid-enriched DAP kinase, regulates the TCR activation threshold during thymocyte selection Int. Immunol., November 1, 2005; 17(11): 1379 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Sommers, J. Lee, K. L. Steiner, J. M. Gurson, C. L. DePersis, D. El-Khoury, C. L. Fuller, E. W. Shores, P. E. Love, and L. E. Samelson Mutation of the phospholipase C-{gamma}1-binding site of LAT affects both positive and negative thymocyte selection J. Exp. Med., April 4, 2005; 201(7): 1125 - 1134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Barbee and J. Alberola-Ila Phosphatidylinositol 3-Kinase Regulates Thymic Exit J. Immunol., February 1, 2005; 174(3): 1230 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. DeRyckere, D. L. Mann, and J. DeGregori Characterization of Transcriptional Regulation During Negative Selection In Vivo J. Immunol., July 15, 2003; 171(2): 802 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Sommers, C.-S. Park, J. Lee, C. Feng, C. L. Fuller, A. Grinberg, J. A. Hildebrand, E. Lacana, R. K. Menon, E. W. Shores, et al. A LAT Mutation That Inhibits T Cell Development Yet Induces Lymphoproliferation Science, June 14, 2002; 296(5575): 2040 - 2043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Raman, F. Blaeser, N. Ho, D. L. Engle, C. B. Williams, and T. A. Chatila Requirement for Ca2+/Calmodulin-Dependent Kinase Type IV/Gr in Setting the Thymocyte Selection Threshold J. Immunol., December 1, 2001; 167(11): 6270 - 6278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Bassiri and S. R. Carding A Requirement for IL-2/IL-2 Receptor Signaling in Intrathymic Negative Selection J. Immunol., May 15, 2001; 166(10): 5945 - 5954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hayden-Martinez, L. P. Kane, and S. M. Hedrick Effects of a Constitutively Active Form of Calcineurin on T Cell Activation and Thymic Selection J. Immunol., October 1, 2000; 165(7): 3713 - 3721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Sommers, R. L. Rabin, A. Grinberg, H. C. Tsay, J. Farber, and P. E. Love A Role for the Tec Family Tyrosine Kinase Txk in T Cell Activation and Thymocyte Selection J. Exp. Med., November 15, 1999; 190(10): 1427 - 1438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Freedman, Q.-H. Liu, S. Somersan, M. I. Kotlikoff, and J. A. Punt Receptor Avidity and Costimulation Specify the Intracellular Ca2+ Signaling Pattern in CD4+CD8+ Thymocytes J. Exp. Med., October 4, 1999; 190(7): 943 - 952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mariathasan, M. F. Bachmann, D. Bouchard, T. Ohteki, and P. S. Ohashi Degree of TCR Internalization and Ca2+ Flux Correlates with Thymocyte Selection J. Immunol., December 1, 1998; 161(11): 6030 - 6037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rincon, A. Whitmarsh, D. D. Yang, L. Weiss, B. Derijard, P. Jayaraj, R. J. Davis, and R. A. Flavell The JNK Pathway Regulates the In Vivo Deletion of Immature CD4+CD8+ Thymocytes J. Exp. Med., November 16, 1998; 188(10): 1817 - 1830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Ferrero, F. Anjuere, I. Azcoitia, T. Renno, H. R. MacDonald, and C. Ardavin Viral Superantigen-Induced Negative Selection of TCR Transgenic CD4+ CD8+ Thymocytes Depends on Activation, but not Proliferation Blood, June 1, 1998; 91(11): 4248 - 4254. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
