| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 156, Issue 11 4377-4383, Copyright © 1996 by American Association of Immunologists
ARTICLES |
DJ Hackam, OD Rotstein, MK Bennett, A Klip, S Grinstein and MF Manolson
Department of Surgery, The Toronto Hospital, Toronto, Ontario, Canada.
Phagosomes formed during ingestion of microorganisms by leukocytes undergo a rapid maturation, generating an acidic, microbicidal organelle. Maturation requires interactions with intracellular vesicles that dock and fuse preferentially with the phagosomal membrane. The basis of specificity of vesiculo-phagosomal interaction has not been elucidated. By contrast, the molecular basis of vesicular fusion in other systems is better understood. At neural synapses, vesicular docking and fusion to the plasma membrane are mediated by a protein complex including syntaxin 1. We explored whether macrophages contain syntaxins, and whether selective fusion of vesicles with the phagosome results from the accumulation of syntaxins in the phagosomal membrane. Isoform-specific Abs were utilized to demonstrate utilized to demonstrate that syntaxins 2, 3, and 4, but not syntaxin 1, are present in murine and human macrophages. Biochemical characterization demonstrated the presence of these syntaxins on microsomes, where they are integral membrane proteins. Subcellular localization using confocal immunofluorescence microscopy demonstrated that syntaxins 3 and 4 are present on the plasma membrane as well as on intracellular vesicles. Importantly, phagosomes isolated by fractionation were shown by immunoblotting to contain syntaxins 2, 3, and 4, suggesting that they may participate in phagosomal maturation. The density of the syntaxins on the phagosomal membrane was found to be comparable with that on the surface membrane. This suggests that preferential fusion of vesicles with the phagosomal membrane is not the result of segregation of the syntaxins to this organelle. Instead, local generation of second messengers in the vicinity of the phagosomal membrane may trigger focal fusion.
This article has been cited by other articles:
|
|
 |
|
  R. J. Anand, S. Dai, S. C. Gribar, W. Richardson, J. W. Kohler, R. A. Hoffman, M. F. Branca, J. Li, X.-H. Shi, C. P. Sodhi, et al. A Role for Connexin43 in Macrophage Phagocytosis and Host Survival after Bacterial Peritoneal Infection J. Immunol., December 15, 2008; 181(12): 8534 - 8543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Anand, S. Dai, C. Rippel, C. Leaphart, F. Qureshi, S. C. Gribar, J. W. Kohler, J. Li, D. B. Stolz, C. Sodhi, et al. Activated macrophages inhibit enterocyte gap junctions via the release of nitric oxide Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G109 - G119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hatsuzawa, T. Tamura, H. Hashimoto, H. Hashimoto, S. Yokoya, M. Miura, H. Nagaya, and I. Wada Involvement of Syntaxin 18, an Endoplasmic Reticulum (ER)-localized SNARE Protein, in ER-mediated Phagocytosis Mol. Biol. Cell, September 1, 2006; 17(9): 3964 - 3977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Kay, R. Z. Murray, J. K. Pagan, and J. L. Stow Cytokine Secretion via Cholesterol-rich Lipid Raft-associated SNAREs at the Phagocytic Cup J. Biol. Chem., April 28, 2006; 281(17): 11949 - 11954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Z. Murray, F. G. Wylie, T. Khromykh, D. A. Hume, and J. L. Stow Syntaxin 6 and Vti1b Form a Novel SNARE Complex, Which Is Up-regulated in Activated Macrophages to Facilitate Exocytosis of Tumor Necrosis Factor-{alpha} J. Biol. Chem., March 18, 2005; 280(11): 10478 - 10483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Bared, C. Buechler, A. Boettcher, R. Dayoub, A. Sigruener, M. Grandl, C. Rudolph, A. Dada, and G. Schmitz Association of ABCA1 with Syntaxin 13 and Flotillin-1 and Enhanced Phagocytosis in Tangier Cells Mol. Biol. Cell, December 1, 2004; 15(12): 5399 - 5407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Collins, A. D. Schreiber, S. Grinstein, and W. S. Trimble Syntaxins 13 and 7 Function at Distinct Steps During Phagocytosis J. Immunol., September 15, 2002; 169(6): 3250 - 3256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Pombo, S. Martin-Verdeaux, B. Iannascoli, J. Le Mao, L. Deriano, J. Rivera, and U. Blank IgE Receptor Type I-dependent Regulation of a Rab3D-associated Kinase. A POSSIBLE LINK IN THE CALCIUM-DEPENDENT ASSEMBLY OF SNARE COMPLEXES J. Biol. Chem., November 9, 2001; 276(46): 42893 - 42900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Garin, R. Diez, S. Kieffer, J.-F. Dermine, S. Duclos, E. Gagnon, R. Sadoul, C. Rondeau, and M. Desjardins The Phagosome Proteome: Insight into Phagosome Functions J. Cell Biol., January 8, 2001; 152(1): 165 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Reed, M. L. Fitzgerald, and J. Polgar Molecular mechanisms of platelet exocytosis: insights into the "secrete" life of thrombocytes Blood, November 15, 2000; 96(10): 3334 - 3342. [Full Text] [PDF]
|
|
|
|
 |
|
 S Duclos, R Diez, J Garin, B Papadopoulou, A Descoteaux, H Stenmark, and M Desjardins Rab5 regulates the kiss and run fusion between phagosomes and endosomes and the acquisition of phagosome leishmanicidal properties in RAW 264.7 macrophages J. Cell Sci., January 10, 2000; 113(19): 3531 - 3541. [Abstract] [PDF]
|
|
|
|
 |
|
 E. S. Gold, D. M. Underhill, N. S. Morrissette, J. Guo, M. A. McNiven, and A. Aderem Dynamin 2 Is Required for Phagocytosis in Macrophages J. Exp. Med., December 20, 1999; 190(12): 1849 - 1856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Flaumenhaft, K. Croce, E. Chen, B. Furie, and B. C. Furie Proteins of the Exocytotic Core Complex Mediate Platelet alpha -Granule Secretion. ROLES OF VESICLE-ASSOCIATED MEMBRANE PROTEIN, SNAP-23, AND SYNTAXIN 4 J. Biol. Chem., January 22, 1999; 274(4): 2492 - 2501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cox, C.-C. Tseng, G. Bjekic, and S. Greenberg A Requirement for Phosphatidylinositol 3-Kinase in Pseudopod Extension J. Biol. Chem., January 15, 1999; 274(3): 1240 - 1247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Hackam, O. D. Rotstein, W.-j. Zhang, S. Gruenheid, P. Gros, and S. Grinstein Host Resistance to Intracellular Infection: Mutation of Natural Resistance-associated Macrophage Protein 1 (Nramp1) Impairs Phagosomal Acidification J. Exp. Med., July 20, 1998; 188(2): 351 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Goyal, R. Singh, E. A. Swietlicki, M. S. Levin, and D. C. Rubin Characterization of rat epimorphin/syntaxin 2 expression suggests a role in crypt-villus morphogenesis Am J Physiol Gastrointest Liver Physiol, July 1, 1998; 275(1): G114 - G124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Hackam, O. D. Rotstein, W.-J. Zhang, N. Demaurex, M. Woodside, O. Tsai, and S. Grinstein Regulation of Phagosomal Acidification. DIFFERENTIAL TARGETING OF Na+/H+ EXCHANGERS, Na+/K+-ATPases, AND VACUOLAR-TYPE H+-ATPases J. Biol. Chem., November 21, 1997; 272(47): 29810 - 29820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. P. Lemons, D. Chen, A. M. Bernstein, M. K. Bennett, and S.W. Whiteheart Regulated Secretion in Platelets: Identification of Elements of the Platelet Exocytosis Machinery Blood, August 15, 1997; 90(4): 1490 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Funato, W. Beron, C. Z. Yang, A. Mukhopadhyay, and P. D. Stahl Reconstitution of Phagosome-Lysosome Fusion in Streptolysin O-permeabilized Cells J. Biol. Chem., June 27, 1997; 272(26): 16147 - 16151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Desjardins, N. Nzala, R Corsini, and C Rondeau Maturation of phagosomes is accompanied by changes in their fusion properties and size-selective acquisition of solute materials from endosomes J. Cell Sci., January 9, 1997; 110(18): 2303 - 2314. [Abstract] [PDF]
|
|
|
|
|
|
A. Bogdanovic, F. Bruckert, T. Morio, and M. Satre A Syntaxin 7 Homologue Is Present in Dictyostelium discoideum Endosomes and Controls Their Homotypic Fusion J. Biol. Chem., November 17, 2000; 275(47): 36691 - 36697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Di, B. Krupa, and D. J. Nelson Calcium-G Protein Interactions in the Regulation of Macrophage Secretion J. Biol. Chem., September 28, 2001; 276(40): 37124 - 37132. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
