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The Journal of Immunology, Vol 156, Issue 11 4259-4265, Copyright © 1996 by American Association of Immunologists
ARTICLES |
MR Rigby, R Bortell, LA Stevens, J Moss, T Kanaitsuka, H Shigeta, JP Mordes, DL Greiner and AA Rossini
The Program in Biomedical Sciences, University of Massachusetts Medical Center, Worcester, MA 01605, USA.
RT6 is a glycosylphosphatidylinositol-linked protein found on the surface of mature rat T lymphocytes. Cells that express RT6 have an immunoregulatory function and modulate the expression of autoimmune diabetes mellitus in the BioBreeding rat. A homologue of the rat RT6 gene, designated Rt6, has been identified in the mouse, but expression of mouse Rt6 protein has not been documented. Rat RT6 is known to be a nicotinamide adenine dinucleotide (NAD+) glycohydrolase. We now report that rat RT6.2 and recombinant mouse Rt6 locus 1 proteins possess auto- ADP ribosylation activity. In addition, mouse Rt6 but not rat RT6, catalyzes the ADP ribosylation of exogenous acceptors such as histones. The ADP-ribosyl-protein bonds in auto-ADP-ribosylated rat RT6.2, auto- ADP-ribosylated mouse Rt6, and ADP-ribosylhistone synthesized by Rt6 were stable to HgCl2 and HCl, but labile to NH2OH, consistent with ADP ribosylarginine linkages. To determine if these enzymatic activities could affect the function of rat T cells, the effect of substrate availability on lymphocyte proliferation was examined. An inverse correlation was observed between NAD+ concentration in the medium and the ability of rat T cells to respond to anti-CD3, ConA, and PMA plus ionomycin. The data suggest that lymphocyte surface ADP ribosyltransferases could be involved in signaling and immunoregulatory processes.
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H. Kawamura, F. Aswad, M. Minagawa, K. Malone, H. Kaslow, F. Koch-Nolte, W. H. Schott, E. H. Leiter, and G. Dennert P2X7 Receptor-Dependent and -Independent T Cell Death Is Induced by Nicotinamide Adenine Dinucleotide J. Immunol., February 15, 2005; 174(4): 1971 - 1979. [Abstract] [Full Text] [PDF]
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L. A. Stevens, C. Bourgeois, R. Bortell, and J. Moss Regulatory Role of Arginine 204 in the Catalytic Activity of Rat Alloantigens ART2a and ART2b J. Biol. Chem., May 23, 2003; 278(22): 19591 - 19596. [Abstract] [Full Text] [PDF]
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R. Bortell, J. Moss, R. C. McKenna, M. R. Rigby, D. Niedzwiecki, L. A. Stevens, W. A. Patton, J. P. Mordes, D. L. Greiner, and A. A. Rossini Nicotinamide Adenine Dinucleotide (NAD) and Its Metabolites Inhibit T Lymphocyte Proliferation: Role of Cell Surface NAD Glycohydrolase and Pyrophosphatase Activities J. Immunol., August 15, 2001; 167(4): 2049 - 2059. [Abstract] [Full Text] [PDF]
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B. Weng, W. C. Thompson, H.-J. Kim, R. L. Levine, and J. Moss Modification of the ADP-ribosyltransferase and NAD Glycohydrolase Activities of a Mammalian Transferase (ADP-ribosyltransferase 5) by Auto-ADP-ribosylation J. Biol. Chem., November 5, 1999; 274(45): 31797 - 31803. [Abstract] [Full Text] [PDF]
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 E. Balducci, K. Horiba, J. Usuki, M. Park, V. J. Ferrans, and J. Moss Selective Expression of RT6 Superfamily in Human Bronchial Epithelial Cells Am. J. Respir. Cell Mol. Biol., September 1, 1999; 21(3): 337 - 346. [Abstract] [Full Text]
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 P.-L. Li, C.-L. Chen, R. Bortell, and W. B. Campbell 11,12-Epoxyeicosatrienoic Acid Stimulates Endogenous Mono-ADP-Ribosylation in Bovine Coronary Arterial Smooth Muscle Circ. Res., August 20, 1999; 85(4): 349 - 356. [Abstract] [Full Text] [PDF]
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I. J. Okazaki and J. Moss Glycosylphosphatidylinositol-anchored and Secretory Isoforms of Mono-ADP-ribosyltransferases J. Biol. Chem., September 11, 1998; 273(37): 23617 - 23620. [Full Text] [PDF]
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E. Lesma, J. Moss, H. B. Brewer, R. Bortell, D. Greiner, J. Mordes, and A. A. Rossini Characterization of High Density Lipoprotein-Bound and Soluble RT6 Released Following Administration of Anti-RT6.1 Monoclonal Antibody J. Immunol., August 1, 1998; 161(3): 1212 - 1219. [Abstract] [Full Text] [PDF]
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J. Moss, L. A. Stevens, E. Cavanaugh, I. J. Okazaki, R. Bortell, T. Kanaitsuka, J. P. Mordes, D. L. Greiner, and A. A. Rossini Characterization of Mouse Rt6.1 NAD:Arginine ADP-ribosyltransferase J. Biol. Chem., February 14, 1997; 272(7): 4342 - 4346. [Abstract] [Full Text] [PDF]
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I. J. Okazaki, H.-J. Kim, and J. Moss Cloning and Characterization of a Novel Membrane-associated Lymphocyte NAD:Arginine ADP-ribosyltransferase J. Biol. Chem., September 6, 1996; 271(36): 22052 - 22057. [Abstract] [Full Text] [PDF]
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