| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 156, Issue 11 4146-4153, Copyright © 1996 by American Association of Immunologists
ARTICLES |
JP Moorman, DA Bobak and CS Hahn
Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA.
The small G-protein Rho regulates the actin microfilament-dependent cytoskeleton. Exoenzyme C3 of Clostridium botulinum ADP-ribosylates Rho at Asn41, a modification that functionally inactivates Rho. Using a Sindbis virus-based transient gene expression system, we studied the role of Rho in murine EL4 T lymphoma cells. We generated a double subgenomic infectious Sindbis virus (dsSIN:C3) recombinant which expressed C3 in >95% of EL4 cells. This intracellular C3 resulted in modification and inactivation of virtually all endogenous Rho. dsSIN:C3 infection led to the formation of multinucleate cells, likely by inhibiting the actin microfilament-dependent step of cytokinesis. Intriguingly, in spite of the inhibition of cytokinesis, karyokinesis continued, with the result that cells containing a nuclear DNA content as high as 16N (eight nuclei) were observed. In addition, dsSIN:C3- mediated inactivation of Rho was a potent activator of apoptosis in EL4 cells. To discern whether the formation of multinucleate cells was responsible for the activation of apoptosis, 5-fluorouracil (5-FUra) was used to induce cell cycle arrest. As expected, EL4 cells treated with 5-FUra were prevented from forming multinucleate cells upon infection with dsSIN:C3. dsSIN:C3 infection, however, still caused marked apoptosis in 5-FUra-treated cells, indicating that this activation of apoptosis was independent of multinucleate cell formation.
This article has been cited by other articles:
|
|
 |
|
  T. Casselli, T. Lynch, C. M. Southward, B. W. Jones, and R. DeVinney Vibrio parahaemolyticus Inhibition of Rho Family GTPase Activation Requires a Functional Chromosome I Type III Secretion System Infect. Immun., May 1, 2008; 76(5): 2202 - 2211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huelsenbeck, S. Dreger, R. Gerhard, H. Barth, I. Just, and H. Genth Difference in the Cytotoxic Effects of Toxin B from Clostridium difficile Strain VPI 10463 and Toxin B from Variant Clostridium difficile Strain 1470 Infect. Immun., February 1, 2007; 75(2): 801 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Su, J. M. Agati, and S. J. Parsons p190RhoGAP is cell cycle regulated and affects cytokinesis J. Cell Biol., November 10, 2003; 163(3): 571 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. I. Dubreuil, M. J. Winton, and L. McKerracher Rho activation patterns after spinal cord injury and the role of activated Rho in apoptosis in the central nervous system J. Cell Biol., July 21, 2003; 162(2): 233 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Liu, F. Di Cunto, S. Imarisio, and L. M. Reid Citron Kinase Is a Cell Cycle-dependent, Nuclear Protein Required for G2/M Transition of Hepatocytes J. Biol. Chem., January 17, 2003; 278(4): 2541 - 2548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hippenstiel, B. Schmeck, P. D. N'Guessan, J. Seybold, M. Krull, K. Preissner, C. V. Eichel-Streiber, and N. Suttorp Rho protein inactivation induced apoptosis of cultured human endothelial cells Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L830 - L838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Litvak, D. Tian, S. Carmon, and S. Lev Nir2, a Human Homolog of Drosophila melanogaster Retinal Degeneration B Protein, Is Essential for Cytokinesis Mol. Cell. Biol., July 15, 2002; 22(14): 5064 - 5075. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Guertin, S. Trautmann, and D. McCollum Cytokinesis in Eukaryotes Microbiol. Mol. Biol. Rev., June 1, 2002; 66(2): 155 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Jantsch-Plunger, P. Gonczy, A. Romano, H. Schnabel, D. Hamill, R. Schnabel, A. A. Hyman, and M. Glotzer CYK-4: A Rho Family GTPase Activating Protein (GAP) Required for Central Spindle Formation and Cytokinesis J. Cell Biol., June 26, 2000; 149(7): 1391 - 1404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Prokopenko, R. Saint, and H. J. Bellen Untying the Gordian Knot of Cytokinesis: Role of Small G Proteins and Their Regulators J. Cell Biol., March 6, 2000; 148(5): 843 - 848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Rawlings, G. M. Crooks, D. Bockstoce, L. W. Barsky, R. Parkman, and K. I. Weinberg Spontaneous Apoptosis in Lymphocytes From Patients With Wiskott-Aldrich Syndrome: Correlation of Accelerated Cell Death and Attenuated Bcl-2 Expression Blood, December 1, 1999; 94(11): 3872 - 3882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Angkachatchai and T. H. Finkel ADP-Ribosylation of Rho by C3 Ribosyltransferase Inhibits IL-2 Production and Sustained Calcium Influx in Activated T Cells J. Immunol., October 1, 1999; 163(7): 3819 - 3825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Meacci, V. Vasta, J. P. Moorman, D. A. Bobak, P. Bruni, J. Moss, and M. Vaughan Effect of Rho and ADP-ribosylation Factor GTPases on Phospholipase D Activity in Intact Human Adenocarcinoma A549 Cells J. Biol. Chem., June 25, 1999; 274(26): 18605 - 18612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. S. Hahn, A. Guanzon, C. M. Rice, and C. S. Hahn Class I MHC Molecule-Mediated Inhibition of Sindbis Virus Replication J. Immunol., January 1, 1999; 162(1): 69 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. R. Mahida, A. Galvin, S. Makh, S. Hyde, L. Sanfilippo, S. P. Borriello, and H. F. Sewell Effect of Clostridium difficile Toxin A on Human Colonic Lamina Propria Cells: Early Loss of Macrophages Followed by T-Cell Apoptosis Infect. Immun., November 1, 1998; 66(11): 5462 - 5469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. P. Coxon, H. L. Benford, R. G. G. Russell, and M. J. Rogers Protein Synthesis Is Required for Caspase Activation and Induction of Apoptosis by Bisphosphonate Drugs Mol. Pharmacol., October 1, 1998; 54(4): 631 - 638. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Guijarro, L. M. Blanco-Colio, M. Ortego, C. Alonso, A. Ortiz, J. J. Plaza, C. Diaz, G. Hernandez, and J. Egido 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase and Isoprenylation Inhibitors Induce Apoptosis of Vascular Smooth Muscle Cells in Culture Circ. Res., September 7, 1998; 83(5): 490 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Li, V. B. G. Segu, M. E. Rabaglia, R.-H. Luo, A. Kowluru, and S. A. Metz Prolonged Depletion of Guanosine Triphosphate Induces Death of Insulin-Secreting Cells by Apoptosis Endocrinology, September 1, 1998; 139(9): 3752 - 3762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Fiorentini, A. Fabbri, L. Falzano, A. Fattorossi, P. Matarrese, R. Rivabene, and G. Donelli Clostridium difficile Toxin B Induces Apoptosis in Intestinal Cultured Cells Infect. Immun., June 1, 1998; 66(6): 2660 - 2665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gauthier-Rouvière, E. Vignal, M. Mériane, P. Roux, P. Montcourier, and P. Fort RhoG GTPase Controls a Pathway That Independently Activates Rac1 and Cdc42Hs Mol. Biol. Cell, June 1, 1998; 9(6): 1379 - 1394. [Abstract] [Full Text]
|
|
|
|
|
|
H. Mellor, P. Flynn, C. D. Nobes, A. Hall, and P. J. Parker PRK1 Is Targeted to Endosomes by the Small GTPase, RhoB J. Biol. Chem., February 27, 1998; 273(9): 4811 - 4814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Perona, S Montaner, L Saniger, I Sanchez-Perez, R Bravo, and J C Lacal Activation of the nuclear factor-kappaB by Rho, CDC42, and Rac-1 proteins. Genes & Dev., February 15, 1997; 11(4): 463 - 475. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
