The Journal of Immunology, Vol 156, Issue 11 4092-4099, Copyright © 1996 by American Association of Immunologists

ARTICLES

CD95 (Fas/Apo-1)-induced apoptosis results in loss of glucose transporter function

MV Berridge, AS Tan, KD McCoy, M Kansara and F Rudert
Malaghan Institute of Medical Research, Wellington School of Medicine, Wellington, New Zealand.

Treatment of activated human T cells with CD95 (Fas/Apo-1) ligand or Abs against CD95 results in apoptotic cell death. Although cellular responses to CD95 ligation have been described in some detail, the early molecular events that result in T cell death are only now beginning to be elucidated. Using Jurkat cells as a model of activated human T cells, we have investigated the effects of CD95 ligation on glucose transport and on glucose transporter function. We show that within minutes of CD95 activation, the ability to transport glucose across the plasma membrane is compromised and that transient exposure to Abs against CD95 for as little as 3 min results in reduced glucose transport and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) responses measured at 16 h. The effects of CD95 ligation on glucose transport are shown to be associated with loss of affinity of glucose transporters for glucose without altered maximum velocity and without changes in the cell surface expression of Glut 1, the predominant glucose transporter isotype on Jurkat cells. These results support a model of CD95 induced cell death that, at least in its early stages, does not depend on signaling to the nucleus or on macromolecular synthesis. Acute regulation of glucose transport is proposed to be an early effector mechanism in CD95-induced apoptotic cell death.

This article has been cited by other articles:

				D. H. Grimm, A. Karihaloo, Y. Cai, S. Somlo, L. G. Cantley, and M. J. Caplan Polycystin-2 Regulates Proliferation and Branching Morphogenesis in Kidney Epithelial Cells J. Biol. Chem., January 6, 2006; 281(1): 137 - 144. [Abstract] [Full Text] [PDF]

				H. El-Daly, M. Kull, S. Zimmermann, M. Pantic, C. F. Waller, and U. M. Martens Selective cytotoxicity and telomere damage in leukemia cells using the telomerase inhibitor BIBR1532 Blood, February 15, 2005; 105(4): 1742 - 1749. [Abstract] [Full Text] [PDF]

				J. Bentley, D. Itchayanan, K. Barnes, E. McIntosh, X. Tang, C. P. Downes, G. D. Holman, A. D. Whetton, P. J. Owen-Lynch, and S. A. Baldwin Interleukin-3-mediated Cell Survival Signals Include Phosphatidylinositol 3-Kinase-dependent Translocation of the Glucose Transporter GLUT1 to the Cell Surface J. Biol. Chem., October 10, 2003; 278(41): 39337 - 39348. [Abstract] [Full Text] [PDF]

				C. Munoz-Pinedo, C. Ruiz-Ruiz, C. Ruiz de Almodovar, C. Palacios, and A. Lopez-Rivas Inhibition of Glucose Metabolism Sensitizes Tumor Cells to Death Receptor-triggered Apoptosis through Enhancement of Death-inducing Signaling Complex Formation and Apical Procaspase-8 Processing J. Biol. Chem., April 4, 2003; 278(15): 12759 - 12768. [Abstract] [Full Text] [PDF]

				M. M.-Y. Chi, A. Hoehn, and K. H. Moley Metabolic changes in the glucose-induced apoptotic blastocyst suggest alterations in mitochondrial physiology Am J Physiol Endocrinol Metab, August 1, 2002; 283(2): E226 - E232. [Abstract] [Full Text] [PDF]

				N. Samih, S. Hovsepian, A. Aouani, D. Lombardo, and G. Fayet Glut-1 Translocation in FRTL-5 Thyroid Cells: Role of Phosphatidylinositol 3-Kinase and N-Glycosylation Endocrinology, November 1, 2000; 141(11): 4146 - 4155. [Abstract] [Full Text] [PDF]

				T. A.D. Smith, N. R. Maisey, J. C. Titley, L. E. Jackson, M. O. Leach, and S. M. Ronen Treatment of SW620 Cells with Tomudex and Oxaliplatin Induces Changes in 2-Deoxy-D-Glucose Incorporation Associated with Modifications in Glucose Transport J. Nucl. Med., October 1, 2000; 41(10): 1753 - 1759. [Abstract] [Full Text] [PDF]

				C. Depre and H. Taegtmeyer Metabolic aspects of programmed cell survival and cell death in the heart Cardiovasc Res, February 1, 2000; 45(3): 538 - 548. [Abstract] [Full Text] [PDF]

				K. Banki, E. Hutter, N. J. Gonchoroff, and A. Perl Elevation of Mitochondrial Transmembrane Potential and Reactive Oxygen Intermediate Levels Are Early Events and Occur Independently from Activation of Caspases in Fas Signaling J. Immunol., February 1, 1999; 162(3): 1466 - 1479. [Abstract] [Full Text] [PDF]

				K. H. Moley, M. M.-Y. Chi, and M. M. Mueckler Maternal hyperglycemia alters glucose transport and utilization in mouse preimplantation embryos Am J Physiol Endocrinol Metab, July 1, 1998; 275(1): E38 - E47. [Abstract] [Full Text] [PDF]

				A. S. Tan, N. Ahmed, and M. V. Berridge Acute Regulation of Glucose Transport After Activation of Human Peripheral Blood Neutrophils by Phorbol Myristate Acetate, fMLP, and Granulocyte-Macrophage Colony-Stimulating Factor Blood, January 15, 1998; 91(2): 649 - 655. [Abstract] [Full Text] [PDF]

				M. M-Y Chi, J. Pingsterhaus, M. Carayannopoulos, and K. H. Moley Decreased Glucose Transporter Expression Triggers BAX-dependent Apoptosis in the Murine Blastocyst J. Biol. Chem., December 15, 2000; 275(51): 40252 - 40257. [Abstract] [Full Text] [PDF]