The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chaturvedi, V.
Right arrow Articles by Newman, S. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chaturvedi, V.
Right arrow Articles by Newman, S. L.

The Journal of Immunology, Vol 156, Issue 10 3836-3840, Copyright © 1996 by American Association of Immunologists

ARTICLES

Oxidative killing of Cryptococcus neoformans by human neutrophils. Evidence that fungal mannitol protects by scavenging reactive oxygen intermediates

V Chaturvedi, B Wong and SL Newman
Department of Internal Medicine, Yale University School of Medicine, Infectious Diseases Section, West Haven, CT 06516, USA.

Polymorphonuclear neutrophils (PMN) kill Cryptococcus neoformans (Cn) by oxidative mechanisms, but the roles of various reactive oxygen intermediates (ROIs) are not known. We used a mannitol low-producing Cn mutant (Cn MLP) and its wild-type parent (Cn H99) to examine the role of ROIs distal to H2O2 in PMN killing and to determine whether mannitol produced by Cn protects the fungus against ROIs. At PMN:Cn cell ratios of 1:1, 10:1, and 100:1, PMN killed significantly more Cn MLP than Cn H99 cells after 2 and 4 h (p less than 0.05). Superoxide dismutase and the hydroxyl radical (OH.) scavengers mannitol and DMSO inhibited killing of both strains (p less than 0.05), but catalase did not. Cn H99 and Cn MLP stimulated PMN to produce similar amounts of O2- and H2O2. In contrast, Cn MLP stimulated greater luminol-dependent chemiluminescence than did Cn H99 (p less than 0.05). Finally, H2O2 alone killed similar numbers of Cn H99 and Cn MLP cells, but oxidants generated by FeSO4 (1 microM), H2O2 (10 microM), and iodide (1 to 3 microM) killed significantly more Cn MLP than Cn H99 cells in 1 h (p less than 0.05). Mannitol, DMSO, and catalase completely inhibited killing of both Cn strains by this cellfree system, but superoxide dismutase did not. These results suggest that 1) distal ROIs such as OH. and HOCI are key effector molecules against Cn, and 2) mannitol produced by Cn may protect against oxidative killing by scavenging distal ROIs.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
R. J. Snelgrove, L. Edwards, A. E. Williams, A. J. Rae, and T. Hussell
In the Absence of Reactive Oxygen Species, T Cells Default to a Th1 Phenotype and Mediate Protection against Pulmonary Cryptococcus neoformans Infection
J. Immunol., October 15, 2006; 177(8): 5509 - 5516.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. L. Wozniak, J. M. Vyas, and S. M. Levitz
In Vivo Role of Dendritic Cells in a Murine Model of Pulmonary Cryptococcosis
Infect. Immun., July 1, 2006; 74(7): 3817 - 3824.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
P. Ren, D. J. Springer, M. J. Behr, W. A. Samsonoff, S. Chaturvedi, and V. Chaturvedi
Transcription Factor STE12{alpha} Has Distinct Roles in Morphogenesis, Virulence, and Ecological Fitness of the Primary Pathogenic Yeast Cryptococcus gattii.
Eukaryot. Cell, July 1, 2006; 5(7): 1065 - 1080.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
G. Kayingo and B. Wong
The MAP kinase Hog1p differentially regulates stress-induced production and accumulation of glycerol and D-arabitol in Candida albicans
Microbiology, September 1, 2005; 151(9): 2987 - 2999.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
X. Shao, A. Mednick, M. Alvarez, N. van Rooijen, A. Casadevall, and D. L. Goldman
An Innate Immune System Cell Is a Major Determinant of Species-Related Susceptibility Differences to Fungal Pneumonia
J. Immunol., September 1, 2005; 175(5): 3244 - 3251.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
H. W. Wisselink, A. P. H. A. Moers, A. E. Mars, M. H. N. Hoefnagel, W. M. de Vos, and J. Hugenholtz
Overproduction of Heterologous Mannitol 1-Phosphatase: a Key Factor for Engineering Mannitol Production by Lactococcus lactis
Appl. Envir. Microbiol., March 1, 2005; 71(3): 1507 - 1514.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. M. Kelly, J. Chen, L. E. Yauch, and S. M. Levitz
Opsonic Requirements for Dendritic Cell-Mediated Responses to Cryptococcus neoformans
Infect. Immun., January 1, 2005; 73(1): 592 - 598.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. T. Voegele, M. Hahn, G. Lohaus, T. Link, I. Heiser, and K. Mendgen
Possible Roles for Mannitol and Mannitol Dehydrogenase in the Biotrophic Plant Pathogen Uromyces fabae
Plant Physiology, January 1, 2005; 137(1): 190 - 198.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
T. A. Missall, J. K. Lodge, and J. E. McEwen
Mechanisms of Resistance to Oxidative and Nitrosative Stress: Implications for Fungal Survival in Mammalian Hosts
Eukaryot. Cell, August 1, 2004; 3(4): 835 - 846.
[Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
H. W. Wisselink, A. E. Mars, P. van der Meer, G. Eggink, and J. Hugenholtz
Metabolic Engineering of Mannitol Production in Lactococcus lactis: Influence of Overexpression of Mannitol 1-Phosphate Dehydrogenase in Different Genetic Backgrounds
Appl. Envir. Microbiol., July 1, 2004; 70(7): 4286 - 4292.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Bellocchio, C. Montagnoli, S. Bozza, R. Gaziano, G. Rossi, S. S. Mambula, A. Vecchi, A. Mantovani, S. M. Levitz, and L. Romani
The Contribution of the Toll-Like/IL-1 Receptor Superfamily to Innate and Adaptive Immunity to Fungal Pathogens In Vivo
J. Immunol., March 1, 2004; 172(5): 3059 - 3069.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
G. J. G. Ruijter, M. Bax, H. Patel, S. J. Flitter, P. J. I. van de Vondervoort, R. P. de Vries, P. A. vanKuyk, and J. Visser
Mannitol Is Required for Stress Tolerance in Aspergillus niger Conidiospores
Eukaryot. Cell, August 1, 2003; 2(4): 690 - 698.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
R. Alonso-Monge, F. Navarro-Garcia, E. Roman, A. I. Negredo, B. Eisman, C. Nombela, and J. Pla
The Hog1 Mitogen-Activated Protein Kinase Is Essential in the Oxidative Stress Response and Chlamydospore Formation in Candidaalbicans
Eukaryot. Cell, April 1, 2003; 2(2): 351 - 361.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
G. M. Cox, T. S. Harrison, H. C. McDade, C. P. Taborda, G. Heinrich, A. Casadevall, and J. R. Perfect
Superoxide Dismutase Influences the Virulence of Cryptococcus neoformans by Affecting Growth within Macrophages
Infect. Immun., January 1, 2003; 71(1): 173 - 180.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. S.-y. Li, F. Chu, A. Reilly, and G. M. Winslow
Antibodies Highly Effective in SCID Mice During Infection by the Intracellular Bacterium Ehrlichia chaffeensis Are of Picomolar Affinity and Exhibit Preferential Epitope and Isotype Utilization
J. Immunol., August 1, 2002; 169(3): 1419 - 1425.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Rivera, J. Mukherjee, L. M. Weiss, and A. Casadevall
Antibody Efficacy in Murine Pulmonary Cryptococcus neoformans Infection: A Role for Nitric Oxide
J. Immunol., April 1, 2002; 168(7): 3419 - 3427.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. L. Nichols, S. K. Bauman, F. B. Schafer, and J. W. Murphy
Differences in Components at Delayed-Type Hypersensitivity Reaction Sites in Mice Immunized with Either a Protective or a Nonprotective Immunogen of Cryptococcus neoformans
Infect. Immun., February 1, 2002; 70(2): 591 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. S. Mambula, E. R. Simons, R. Hastey, M. E. Selsted, and S. M. Levitz
Human Neutrophil-Mediated Nonoxidative Antifungal Activity against Cryptococcus neoformans
Infect. Immun., November 1, 2000; 68(11): 6257 - 6264.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
K. Suvarna, A. Bartiss, and B. Wong
Mannitol-1-phosphate dehydrogenase from Cryptococcus neoformans is a zinc-containing long-chain alcohol/polyol dehydrogenase
Microbiology, October 1, 2000; 146(10): 2705 - 2713.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
L. Liu, R. P. Tewari, and P. R. Williamson
Laccase Protects Cryptococcus neoformans from Antifungal Activity of Alveolar Macrophages
Infect. Immun., November 1, 1999; 67(11): 6034 - 6039.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. A. Noventa-Jordão, R. M. Couto, M. H. S. Goldman, J. Aguirre, S. Iyer, A. Caplan, H. F. Terenzi, and G. H. Goldman
Catalase activity is necessary for heat-shock recovery in Aspergillus nidulans germlings
Microbiology, November 1, 1999; 145(11): 3229 - 3234.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
W. Pattanagul and M. A. Madore
Water Deficit Effects on Raffinose Family Oligosaccharide Metabolism in Coleus
Plant Physiology, November 1, 1999; 121(3): 987 - 993.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
K. J. Nyhus and E. S. Jacobson
Genetic and Physiologic Characterization of Ferric/Cupric Reductase Constitutive Mutants of Cryptococcus neoformans
Infect. Immun., May 1, 1999; 67(5): 2357 - 2365.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. B. Jennings, M. Ehrenshaft, D. M. Pharr, and J. D. Williamson
Roles for mannitol and mannitol dehydrogenase in active oxygen-mediated plant defense
PNAS, December 8, 1998; 95(25): 15129 - 15133.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Benaroudj, D. H. Lee, and A. L. Goldberg
Trehalose Accumulation during Cellular Stress Protects Cells and Cellular Proteins from Damage by Oxygen Radicals
J. Biol. Chem., June 22, 2001; 276(26): 24261 - 24267.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.