| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The Journal of Immunology, Vol 156, Issue 10 3621-3630, Copyright © 1996 by American Association of Immunologists
ARTICLES |
CR Engwerda, BS Fox and BS Handwerger
Division of Rheumatology and Clinical Immunology, Department of Medicine, University of Maryland at Baltimore 21201, USA.
We previously have shown that T cell proliferation in response to a primary signal through the CD3 epsilon chain and a costimulatory signal via the CD28 molecule is impaired in healthy, aged mice. To determine whether age-related alterations in cytokine production might explain the reduced proliferative responses of T cells from aged mice, we examined the secretion of the major T cell immunoregulatory cytokines, IFN-gamma, IL-4, and IL-2. Splenic T cells from young (2 to 4 mo) and aged (20 to 26 mo) mice were studied. T cells were stimulated with immobilized anti-CD3 epsilon chain mAb and soluble anti-CD28 mAb for 24 h. T cells from aged mice, when compared with young controls, showed increased IFN-gamma production, no difference in IL-4 production, and decreased IL-2 production. Most IFN-gamma was produced by CD8+ T cells, whereas most IL-2 and IL-4 was produced by CD4+ T cells. Both CD4+ and CD8+ T cells from aged mice produced significantly more IFN-gamma than corresponding cells from young mice. This increased production could be accounted for by increased numbers of CD4+CD44high and CD8+CD44high T cells in aged animals. CD4+CD44high and CD8+CD44high T cells from young mice produced comparable amounts of IFN-gamma as corresponding cells from aged mice. In contrast to unseparated splenic T cells, no age- related difference in IL-2 or IL-4 production by purified CD4+ T cells was observed. Similarly, when CD4+ T cells were further separated into CD44low and CD44high subpopulations, no age-related difference in IL-2 production was found. Therefore, we found no consistent evidence that diminished production of the major T cell growth factors, IL-2 and IL- 4, is responsible for the age-related decrease in the proliferation of T cell subpopulations that were stimulated in vitro through the CD3 epsilon chain and costimulated via the CD28 molecule. The physiologic relevance of increased IFN-gamma production by T cells from aged mice is unknown.
This article has been cited by other articles:
|
|
 |
|
  A. L. Dominguez and J. Lustgarten Implications of Aging and Self-Tolerance on the Generation of Immune and Antitumor Immune Responses Cancer Res., July 1, 2008; 68(13): 5423 - 5431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Frasca, A. M. Landin, R. L. Riley, and B. B. Blomberg Mechanisms for Decreased Function of B Cells in Aged Mice and Humans J. Immunol., March 1, 2008; 180(5): 2741 - 2746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhao, L. Sun, H. Wang, H. Ma, G. Liu, and Y. Zhao Changes of CD4+CD25+Foxp3+ regulatory T cells in aged Balb/c mice J. Leukoc. Biol., June 1, 2007; 81(6): 1386 - 1394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Kim and A. E. Nel The Role of Phase II Antioxidant Enzymes in Protecting Memory T Cells from Spontaneous Apoptosis in Young and Old Mice J. Immunol., September 1, 2005; 175(5): 2948 - 2959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Soda, Y. Kano, T. Nakamura, K. Kasono, M. Kawakami, and F. Konishi Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte J. Immunol., July 1, 2005; 175(1): 237 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lustgarten, A. L. Dominguez, and M. Thoman Aged Mice Develop Protective Antitumor Immune Responses with Appropriate Costimulation J. Immunol., October 1, 2004; 173(7): 4510 - 4515. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ehrchen, A. Sindrilaru, S. Grabbe, F. Schonlau, C. Schlesiger, C. Sorg, K. Scharffetter-Kochanek, and C. Sunderkotter Senescent BALB/c Mice Are Able To Develop Resistance to Leishmania major Infection Infect. Immun., September 1, 2004; 72(9): 5106 - 5114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Jayashankar, K. M. Brasky, J. A. Ward, and R. Attanasio Lymphocyte Modulation in a Baboon Model of Immunosenescence Clin. Vaccine Immunol., September 1, 2003; 10(5): 870 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Li, Z. Cai, W. Shi, A. Brunmark, M. Jackson, and P.-J. Linton Early Antigen-Specific Response by Naive CD8 T Cells Is Not Altered with Aging J. Immunol., June 15, 2002; 168(12): 6120 - 6127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Johnson, S. J. Rozzo, and J. C. Cambier Aging-Dependent Exclusion of Antigen-Inexperienced Cells from the Peripheral B Cell Repertoire J. Immunol., May 15, 2002; 168(10): 5014 - 5023. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sanchez, K. Lindroth, E. Sverremark, A. G. Fernandez, and C. Fernandez The response in old mice: positive and negative immune memory after priming in early age Int. Immunol., October 1, 2001; 13(10): 1213 - 1221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ohkusu-Tsukada, T. Tsukada, and K.-i. Isobe Accelerated Development and Aging of the Immune System in p53-Deficient Mice J. Immunol., August 15, 1999; 163(4): 1966 - 1972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Timm and M. L. Thoman Maturation of CD4+ Lymphocytes in the Aged Microenvironment Results in a Memory-Enriched Population J. Immunol., January 15, 1999; 162(2): 711 - 717. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
