The Journal of Immunology, Vol 156, Issue 1 35-41, Copyright © 1996 by American Association of Immunologists

ARTICLES

Activated alpha beta-CD8+, but not alpha alpha-CD8+, TCR-alpha beta+ murine intestinal intraepithelial lymphocytes can mediate perforin- based cytotoxicity, whereas both subsets are active in Fas-based cytotoxicity

V Gelfanov, V Gelfanova, YG Lai and NS Liao
Institute of Molecular Biology, Academia Sinica, Taiwan, Republic of China.

CD8 single-positive (CD8+) T cells in murine intestinal intraepithelial lymphocytes (iIEL) consist of alpha alpha-CD8+ and alpha beta-CD8+ subpopulations. Cytotoxicity represents an important function of peripheral CD8+ T cells, so we examined perforin-granzymebased and Fas- based cytotoxicity of activated CD8+ TCR-alpha beta+ iIEL subsets. We found that allospecific CTL activity was induced from alpha beta-CD8+ iIEL but not from alpha alpha-CD8+ iIEL even when allospecific TCR were present on the iIEL, as demonstrated by using 2C TCR transgenic mice. On the other hand, both CD8+ iIEL subsets proliferated upon allostimulation with a lower responder frequency than CD8+ LN cells. The alpha alpha-CD8+ TCR-alpha beta+ iIEL appeared to lose their ability to perform perforin-based killing after activation through TCR because fresh cells lysed P815 cells coated with anti-TCR beta-chain (TCR-beta) mAb, whereas cells activated by plate-bound anti-TCR mAb did not. Of interest, both activated CD8+ TCR-alpha beta+ iIEL subsets, but not fresh cells, were able to mediate Fas-based killing when triggered with PMA and CA2+ ionophore.

This article has been cited by other articles:

				H. Yang, Y. Fan, and D. H. Teitelbaum Intraepithelial lymphocyte-derived interferon-gamma evokes enterocyte apoptosis with parenteral nutrition in mice Am J Physiol Gastrointest Liver Physiol, April 1, 2003; 284(4): G629 - G637. [Abstract] [Full Text] [PDF]

				A Di Sabatino, R Ciccocioppo, S D'Alo, R Parroni, D Millimaggi, M G Cifone, and G R Corazza Intraepithelial and lamina propria lymphocytes show distinct patterns of apoptosis whereas both populations are active in Fas based cytotoxicity in coeliac disease Gut, September 1, 2001; 49(3): 380 - 386. [Abstract] [Full Text] [PDF]

				L. H. Kasper and D. Buzoni-Gatel Ups and Downs of Mucosal Cellular Immunity against Protozoan Parasites Infect. Immun., January 1, 2001; 69(1): 1 - 8. [Full Text] [PDF]

				A. Muehlhoefer, L. J. Saubermann, X. Gu, K. Luedtke-Heckenkamp, R. Xavier, R. S. Blumberg, D. K. Podolsky, R. P. MacDermott, and H.-C. Reinecker Fractalkine Is an Epithelial and Endothelial Cell-Derived Chemoattractant for Intraepithelial Lymphocytes in the Small Intestinal Mucosa J. Immunol., March 15, 2000; 164(6): 3368 - 3376. [Abstract] [Full Text] [PDF]

				N. Corazza, S. Muller, T. Brunner, D. Kagi, and C. Mueller Differential Contribution of Fas- and Perforin-Mediated Mechanisms to the Cell-Mediated Cytotoxic Activity of Naive and In Vivo-Primed Intestinal Intraepithelial Lymphocytes J. Immunol., January 1, 2000; 164(1): 398 - 403. [Abstract] [Full Text] [PDF]

				Y.-G. Lai, V. Gelfanov, V. Gelfanova, L. Kulik, C.-L. Chu, S.-W. Jeng, and N.-S. Liao IL-15 Promotes Survival But Not Effector Function Differentiation of CD8+ TCR{alpha}{beta}+ Intestinal Intraepithelial Lymphocytes J. Immunol., December 1, 1999; 163(11): 5843 - 5850. [Abstract] [Full Text] [PDF]

				D. Buzoni-Gatel, H. Debbabi, M. Moretto, I. H. Dimier-Poisson, A. C. Lepage, D. T. Bout, and L. H. Kasper Intraepithelial Lymphocytes Traffic to the Intestine and Enhance Resistance to Toxoplasma gondii Oral Infection J. Immunol., May 15, 1999; 162(10): 5846 - 5852. [Abstract] [Full Text] [PDF]

				T.-J. Lin, N. Hirji, O. Nohara, G. R. Stenton, M. Gilchrist, and A. D. Befus Mast Cells Express Novel CD8 Molecules That Selectively Modulate Mediator Secretion J. Immunol., December 1, 1998; 161(11): 6265 - 6272. [Abstract] [Full Text] [PDF]

				A. C. Lepage, D. Buzoni-Gatel, D. T. Bout, and L. H. Kasper Gut-Derived Intraepithelial Lymphocytes Induce Long Term Immunity Against Toxoplasma gondii J. Immunol., November 1, 1998; 161(9): 4902 - 4908. [Abstract] [Full Text] [PDF]