| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 156, Issue 1 297-309, Copyright © 1996 by American Association of Immunologists
ARTICLES |
AL Kindzelskii, ZO Laska, RF Todd 3rd and HR Petty
Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA.
Previous studies have shown that the leukocyte integrin CR3 (CD11b/CD18) is physically associated with the urokinase-type plasminogen activator receptor (uPAR;CD87), a glycosyl- phosphatidylinositol (GPI)-linked protein, in resting neutrophil membranes. We now show that uPAR-to-CR3 interactions are reversible, correlating with cell shape. Neutrophils were first labeled with fluorescein conjugates of anti-CR3 F(ab')2 fragments followed by capping using a second-step F(ab')2 directed against murine F(ab')2s. Cells were then probed using rhodamine-conjugated anti-uPAR F(ab')2s. Although uPAR co-caps with CR3 on resting cells, uPAR was found to dissociate or "uncap" coincident with spontaneous cell polarization for migration. CR3 caps transformed into uropods while uPAR accumulated at lamellipodia of polarized cells. Capping was unnecessary for the observed distribution of CR3 and uPAR since the anti-CR3 and anti-uPAR F(ab')2s traffic to the uropod and lamellipodium, respectively, during polarization of uncapped cells. These receptors reassociate when cells return to a spherical morphology. In contrast to uPAR, Fc gamma RIIIB did not dissociate from CR3 caps during cell polarization. Resonance energy transfer (RET) microscopy was used to image the spatial distribution of RET and to follow the kinetics of association and dissociation. Initial levels of RET dramatically fell during cell polarization, but did not change on cells fixed with paraformaldehyde. Receptor reassociation was a biphasic process with initial reassociation about the perimeter of a cap, followed by a plateau and a slower rise in RET within a cap. We suggest that cells regulate receptor-receptor associations depending upon their physiologic activities.
This article has been cited by other articles:
|
|
 |
|
  M. A. Kiskowski and A. K. Kenworthy In Silico Characterization of Resonance Energy Transfer for Disk-Shaped Membrane Domains Biophys. J., May 1, 2007; 92(9): 3040 - 3051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Ng and D. E. Griffin Acid Sphingomyelinase Deficiency Increases Susceptibility to Fatal Alphavirus Encephalomyelitis J. Virol., November 15, 2006; 80(22): 10989 - 10999. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bagchi, Z. Liao, F. A. Gonzalez, N. E. Chorna, C. I. Seye, G. A. Weisman, and L. Erb The P2Y2 Nucleotide Receptor Interacts with {alpha}v Integrins to Activate Go and Induce Cell Migration J. Biol. Chem., November 25, 2005; 280(47): 39050 - 39057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Mattila, C. E. Green, U. Schaff, S. I. Simon, and B. Walcheck Cytoskeletal interactions regulate inducible L-selectin clustering Am J Physiol Cell Physiol, August 1, 2005; 289(2): C323 - C332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bass, F. Werner, E. Odintsova, T. Sugiura, F. Berditchevski, and V. Ellis Regulation of Urokinase Receptor Proteolytic Function by the Tetraspanin CD82 J. Biol. Chem., April 15, 2005; 280(15): 14811 - 14818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Vorup-Jensen, C. V. Carman, M. Shimaoka, P. Schuck, J. Svitel, and T. A. Springer Exposure of acidic residues as a danger signal for recognition of fibrinogen and other macromolecules by integrin {alpha}X{beta}2 PNAS, February 1, 2005; 102(5): 1614 - 1619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Atarashi, T. Hirata, M. Matsumoto, N. Kanemitsu, and M. Miyasaka Rolling of Th1 Cells via P-Selectin Glycoprotein Ligand-1 Stimulates LFA-1-Mediated Cell Binding to ICAM-1 J. Immunol., February 1, 2005; 174(3): 1424 - 1432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Bernstein, R. S. Greenberg, L. Taliana, and S. K. Masur Urokinase Anchors uPAR to the Actin Cytoskeleton Invest. Ophthalmol. Vis. Sci., September 1, 2004; 45(9): 2967 - 2977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Frasch, P. M. Henson, K. Nagaosa, M. B. Fessler, N. Borregaard, and D. L. Bratton Phospholipid Flip-Flop and Phospholipid Scramblase 1 (PLSCR1) Co-localize to Uropod Rafts in Formylated Met-Leu-Phe-stimulated Neutrophils J. Biol. Chem., April 23, 2004; 279(17): 17625 - 17633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Sitrin, D. R. Johnson, P. M. Pan, D. M. Harsh, J. Huang, H. R. Petty, and R. A. Blackwood Lipid Raft Compartmentalization of Urokinase Receptor Signaling in Human Neutrophils Am. J. Respir. Cell Mol. Biol., February 1, 2004; 30(2): 233 - 241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Rarok, P. C. Limburg, and C. G. M. Kallenberg Neutrophil-activating potential of antineutrophil cytoplasm autoantibodies J. Leukoc. Biol., July 1, 2003; 74(1): 3 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Capo, A. Moynault, Y. Collette, D. Olive, E. J. Brown, D. Raoult, and J.-L. Mege Coxiella burnetii Avoids Macrophage Phagocytosis by Interfering with Spatial Distribution of Complement Receptor 3 J. Immunol., April 15, 2003; 170(8): 4217 - 4225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Pluskota, D. A. Soloviev, and E. F. Plow Convergence of the adhesive and fibrinolytic systems: recognition of urokinase by integrin alpha Mbeta 2 as well as by the urokinase receptor regulates cell adhesion and migration Blood, February 15, 2003; 101(4): 1582 - 1590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xia, G. Borland, J. Huang, I. F. Mizukami, H. R. Petty, R. F. Todd III, and G. D. Ross Function of the Lectin Domain of Mac-1/Complement Receptor Type 3 (CD11b/CD18) in Regulating Neutrophil Adhesion J. Immunol., December 1, 2002; 169(11): 6417 - 6426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Rijneveld, M. Levi, S. Florquin, P. Speelman, P. Carmeliet, and T. van der Poll Urokinase Receptor Is Necessary for Adequate Host Defense Against Pneumococcal Pneumonia J. Immunol., April 1, 2002; 168(7): 3507 - 3511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Seveau, R. J. Eddy, F. R. Maxfield, and L. M. Pierini Cytoskeleton-dependent Membrane Domain Segregation during Neutrophil Polarization Mol. Biol. Cell, November 1, 2001; 12(11): 3550 - 3562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. B. Siconolfi and N. W. Seeds Induction of the Plasminogen Activator System Accompanies Peripheral Nerve Regeneration after Sciatic Nerve Crush J. Neurosci., June 15, 2001; 21(12): 4336 - 4347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Siconolfi and N. W. Seeds Mice Lacking tPA, uPA, or Plasminogen Genes Showed Delayed Functional Recovery after Sciatic Nerve Crush J. Neurosci., June 15, 2001; 21(12): 4348 - 4355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rosenspire, A. Kindzelskii, and H. Petty Pulsed DC electric fields couple to natural NAD(P)H oscillations in HT-1080 fibrosarcoma cells J. Cell Sci., January 4, 2001; 114(8): 1515 - 1520. [Abstract] [PDF]
|
|
|
|
|
|
Q. Jiang, S. Akashi, K. Miyake, and H. R. Petty Cutting Edge: Lipopolysaccharide Induces Physical Proximity Between CD14 and Toll-Like Receptor 4 (TLR4) Prior to Nuclear Translocation of NF-{kappa}B J. Immunol., October 1, 2000; 165(7): 3541 - 3544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Sitrin, P. M. Pan, H. A. Harper, R. F. Todd III, D. M. Harsh, and R. A. Blackwood Clustering of Urokinase Receptors (uPAR; CD87) Induces Proinflammatory Signaling in Human Polymorphonuclear Neutrophils J. Immunol., September 15, 2000; 165(6): 3341 - 3349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pampfer, S. Cordi, S. Cikos, B. Picry, I. Vanderheyden, and R. D. Hertogh Activation of Nuclear Factor {kappa}B and Induction of Apoptosis by Tumor Necrosis Factor-{alpha} in the Mouse Uterine Epithelial WEG-1 Cell Line Biol Reprod, September 1, 2000; 63(3): 879 - 886. [Abstract] [Full Text]
|
|
|
|
|
|
H. Ryu, J.-H. Lee, K. S. Kim, S.-M. Jeong, P.-H. Kim, and H.-T. Chung Regulation of Neutrophil Adhesion by Pituitary Growth Hormone Accompanies Tyrosine Phosphorylation of Jak2, p125FAK, and Paxillin J. Immunol., August 15, 2000; 165(4): 2116 - 2123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Manna, N. K. Sah, and B. B. Aggarwal Protein Tyrosine Kinase p56lck Is Required for Ceramide-induced but Not Tumor Necrosis Factor-induced Activation of NF-kappa B, AP-1, JNK, and Apoptosis J. Biol. Chem., April 28, 2000; 275(18): 13297 - 13306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Manna, A. Mukhopadhyay, and B. B. Aggarwal Human Chorionic Gonadotropin Suppresses Activation of Nuclear Transcription Factor-kappa B and Activator Protein-1 Induced by Tumor Necrosis Factor J. Biol. Chem., April 28, 2000; 275(18): 13307 - 13314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. W. Smith Introduction: functional polarity of motile neutrophils Blood, April 15, 2000; 95(8): 2459 - 2461. [Full Text] [PDF]
|
|
|
|
|
|
D. I. Simon, Y. Wei, L. Zhang, N. K. Rao, H. Xu, Z. Chen, Q. Liu, S. Rosenberg, and H. A. Chapman Identification of a Urokinase Receptor-Integrin Interaction Site. PROMISCUOUS REGULATOR OF INTEGRIN FUNCTION J. Biol. Chem., March 31, 2000; 275(14): 10228 - 10234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Sitrin, P. M. Pan, H. A. Harper, R. A. Blackwood, and R. F. Todd III Urokinase Receptor (CD87) Aggregation Triggers Phosphoinositide Hydrolysis and Intracellular Calcium Mobilization in Mononuclear Phagocytes J. Immunol., December 1, 1999; 163(11): 6193 - 6200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Mallampalli, E. J. Peterson, A. B. Carter, R. G. Salome, S. N. Mathur, and G. A. Koretzky TNF-alpha increases ceramide without inducing apoptosis in alveolar type II epithelial cells Am J Physiol Lung Cell Mol Physiol, March 1, 1999; 276(3): L481 - L490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-L. Guo, B. Kang, L.-J. Yang, and J. R. Williamson Tumor necrosis factor-alpha and ceramide induce cell death through different mechanisms in rat mesangial cells Am J Physiol Renal Physiol, March 1, 1999; 276(3): F390 - F397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Schwarzer, R. Nost, J. Seybold, S. K. Parida, O. Fuhrmann, M. Krull, R. Schmidt, R. Newton, S. Hippenstiel, E. Domann, et al. Two Distinct Phospholipases C of Listeria monocytogenes Induce Ceramide Generation, Nuclear Factor-{kappa}B Activation, and E-Selectin Expression in Human Endothelial Cells J. Immunol., September 15, 1998; 161(6): 3010 - 3018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.K. Kenworthy and M. Edidin Distribution of a Glycosylphosphatidylinositol-anchored Protein at the Apical Surface of MDCK Cells Examined at a Resolution of <100 A Using Imaging Fluorescence Resonance Energy Transfer J. Cell Biol., July 13, 1998; 142(1): 69 - 84. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Avni, Z. Pur, E. Yefenof, and M. Baniyash Complement Receptor 3 of Macrophages Is Associated with Galectin-1-Like Protein J. Immunol., June 15, 1998; 160(12): 6151 - 6158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Harada-Shiba, M. Kinoshita, H. Kamido, and K. Shimokado Oxidized Low Density Lipoprotein Induces Apoptosis in Cultured Human Umbilical Vein Endothelial Cells by Common and Unique Mechanisms J. Biol. Chem., April 17, 1998; 273(16): 9681 - 9687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-C. Hsu, C.-C. Wu, T.-Y. Luh, C.-K. Chou, S.-H. Han, and M.-Z. Lai Apoptotic Signal of Fas Is Not Mediated by Ceramide Blood, April 15, 1998; 91(8): 2658 - 2663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Yorek, J. A. Dunlap, M. J. Thomas, P. R. Cammarata, C. Zhou, and W. L. Lowe Jr. Effect of TNF-alpha on SMIT mRNA levels and myo-inositol accumulation in cultured endothelial cells Am J Physiol Cell Physiol, January 1, 1998; 274(1): C58 - C71. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Plesner, N. Behrendt, and M. Ploug Structure, Function and Expression on Blood and Bone Marrow Cells of the Urokinase-Type Plasminogen Activator Receptor, uPAR Stem Cells, November 1, 1997; 15(6): 398 - 408. [Abstract] [Full Text]
|
|
|
|
|
|
A. M. Weaver, I. M. Hussaini, A. Mazar, J. Henkin, and S. L. Gonias Embryonic Fibroblasts That Are Genetically Deficient in Low Density Lipoprotein Receptor-related Protein Demonstrate Increased Activity of the Urokinase Receptor System and Accelerated Migration on Vitronectin J. Biol. Chem., May 30, 1997; 272(22): 14372 - 14379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Franco, C. Iaccarino, F. Chiaradonna, A. Brandazza, C. Iavarone, M. R. Mastronicola, M. L. Nolli, and M. P. Stoppelli Phosphorylation of Human Pro-Urokinase on Ser138/303 Impairs Its Receptor-dependent Ability to Promote Myelomonocytic Adherence and Motility J. Cell Biol., May 5, 1997; 137(3): 779 - 791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Bonavaud, C Charriere-Bertrand, C Rey, M. Leibovitch, N Pedersen, E Frisdal, E Planus, F Blasi, R Gherardi, and G Barlovatz-Meimon Evidence of a non-conventional role for the urokinase tripartite complex (uPAR/uPA/PAI-1) in myogenic cell fusion J. Cell Sci., January 5, 1997; 110(9): 1083 - 1089. [Abstract] [PDF]
|
|
|
|
|
|
M. Yebra, G. C.N. Parry, S. Stromblad, N. Mackman, S. Rosenberg, B. M. Mueller, and D. A. Cheresh Requirement of Receptor-bound Urokinase-type Plasminogen Activator for Integrin alpha vbeta 5-directed Cell Migration J. Biol. Chem., November 15, 1996; 271(46): 29393 - 29399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wei, M. Lukashev, D. I. Simon, S. C. Bodary, S. Rosenberg, M. V. Doyle, and H. A. Chapman Regulation of Integrin Function by the Urokinase Receptor Science, September 13, 1996; 273(5281): 1551 - 1555. [Abstract]
|
|
|
|
|
|
S. Bulotta, R. Barsacchi, D. Rotiroti, N. Borgese, and E. Clementi Activation of the Endothelial Nitric-oxide Synthase by Tumor Necrosis Factor-alpha . A NOVEL FEEDBACK MECHANISM REGULATING CELL DEATH J. Biol. Chem., February 23, 2001; 276(9): 6529 - 6536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. De Nadai, P. Sestili, O. Cantoni, J.-P. Lievremont, C. Sciorati, R. Barsacchi, S. Moncada, J. Meldolesi, and E. Clementi Nitric oxide inhibits tumor necrosis factor-alpha -induced apoptosis by reducing the generation of ceramide PNAS, May 9, 2000; 97(10): 5480 - 5485. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
