The Journal of Immunology, Vol 156, Issue 1 289-296, Copyright © 1996 by American Association of Immunologists

ARTICLES

Analysis of bovine gamma delta T cell interactions with E-, P-, and L- selectin. Characterization of lymphocyte on lymphocyte rolling and the effects of O-glycoprotease

MA Jutila and S Kurk
Veterinary Molecular Biology, Montana State University, Bozeman 59717, USA.

In earlier studies, we found that bovine gamma delta T cells avidly bind and roll under physiologic flow on E- and P-selection. In this study, we have extended our analyses of shear interactions of gamma delta T cells and have found that once gamma delta T cells permanently bind to endothelial cell monolayers they can support continued rolling of newly arriving gamma delta T cells. Anti-L-selection Abs blocked gamma delta T cell rolling on immobilized gamma delta T cells, and it was L-selection on the rolling cell, not the bound cell, that mediated the interaction. We propose, as we have for neutrophils, that lymphocyte/lymphocyte rolling represents a mechanism for amplification of the extravasation process. Having defined P-, E-, and L-selectin interactions with gamma delta T cells, we tested the susceptibility of the ligands for these selectins to treatment with O-glycoprotease, a compound that selectively cleaves mucin-like selectin ligands. O- glycoprotease treatment of gamma delta T cells blocked their interaction with P-selectin, but not E-selectin. Rolling of O- glycoprotease-treated cells on bound untreated gamma delta T cells was unaffected; however, O-glycoprotease treatment of the bound gamma delta T cells blocked their capacity to support rolling interactions. These results define a new lymphocyte/selectin interaction that may be important in amplifying lymphocyte extravasation and suggest that gamma delta T cell ligands for P- and E-selectin are distinct, whereas ligands for P- and L-selectin may be similar.

This article has been cited by other articles:

				J. G. Clark, J. B. Mandac-Dy, A. E. Dixon, D. K. Madtes, K. M. Burkhart, J. M. Harlan, and D. C. Bullard Trafficking of Th1 Cells to Lung: A Role for Selectins and a P-Selectin Glycoprotein-1-Independent Ligand Am. J. Respir. Cell Mol. Biol., February 1, 2004; 30(2): 220 - 227. [Abstract] [Full Text] [PDF]

				N. Meissner, J. Radke, J. F. Hedges, M. White, M. Behnke, S. Bertolino, M. Abrahamsen, and M. A. Jutila Serial Analysis of Gene Expression in Circulating {gamma}{delta} T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles J. Immunol., January 1, 2003; 170(1): 356 - 364. [Abstract] [Full Text] [PDF]

				A. C. Issekutz and T. B. Issekutz The Role of E-Selectin, P-Selectin, and Very Late Activation Antigen-4 in T Lymphocyte Migration to Dermal Inflammation J. Immunol., February 15, 2002; 168(4): 1934 - 1939. [Abstract] [Full Text] [PDF]

				J. G. Leid, D. A. Steeber, T. F. Tedder, and M. A. Jutila Antibody Binding to a Conformation-Dependent Epitope Induces L-Selectin Association with the Detergent-Resistant Cytoskeleton J. Immunol., April 15, 2001; 166(8): 4899 - 4907. [Abstract] [Full Text] [PDF]

				T. Hirata, G. Merrill-Skoloff, M. Aab, J. Yang, B. C. Furie, and B. Furie P-Selectin Glycoprotein Ligand 1 (PSGL-1) Is a Physiological Ligand for E-Selectin in Mediating T Helper 1 Lymphocyte Migration J. Exp. Med., December 4, 2000; 192(11): 1669 - 1676. [Abstract] [Full Text] [PDF]

				A. J. Young, W. L. Marston, and L. Dudler Subset-Specific Regulation of the Lymphatic Exit of Recirculating Lymphocytes In Vivo J. Immunol., September 15, 2000; 165(6): 3168 - 3174. [Abstract] [Full Text] [PDF]

				J. Yang, T. Hirata, K. Croce, G. Merrill-Skoloff, B. Tchernychev, E. Williams, R. Flaumenhaft, B. C. Furie, and B. Furie Targeted Gene Disruption Demonstrates That P-Selectin Glycoprotein Ligand 1 (PSGL-1) Is Required for P-Selectin-mediated but Not E-Selectin-mediated Neutrophil Rolling and Migration J. Exp. Med., December 20, 1999; 190(12): 1769 - 1782. [Abstract] [Full Text] [PDF]

				L. Tu, P. G. Murphy, X. Li, and T. F. Tedder L-Selectin Ligands Expressed by Human Leukocytes Are HECA-452 Antibody-Defined Carbohydrate Epitopes Preferentially Displayed by P-Selectin Glycoprotein Ligand-1 J. Immunol., November 1, 1999; 163(9): 5070 - 5078. [Abstract] [Full Text] [PDF]

				E. Wilson, M. K. Aydintug, and M. A. Jutila A Circulating Bovine {gamma}{delta} T Cell Subset, Which Is Found in Large Numbers in the Spleen, Accumulates Inefficiently in an Artificial Site of Inflammation: Correlation with Lack of Expression of E-Selectin Ligands and L-Selectin J. Immunol., April 15, 1999; 162(8): 4914 - 4919. [Abstract] [Full Text] [PDF]

				R. Alon, S. Chen, R. Fuhlbrigge, K. D. Puri, and T. A. Springer The kinetics and shear threshold of transient and rolling interactions of L-selectin with its ligand on leukocytes PNAS, September 29, 1998; 95(20): 11631 - 11636. [Abstract] [Full Text] [PDF]

				Y.-C. Lim, K. Snapp, G. S. Kansas, R. Camphausen, H. Ding, and F. W. Luscinskas Important Contributions of P-Selectin Glycoprotein Ligand-1-Mediated Secondary Capture to Human Monocyte Adhesion to P-Selectin, E-Selectin, and TNF-{alpha}-Activated Endothelium Under Flow In Vitro J. Immunol., September 1, 1998; 161(5): 2501 - 2508. [Abstract] [Full Text] [PDF]

				M. A. Jutila, E. Wilson, and S. Kurk Characterization of an Adhesion Molecule that Mediates Leukocyte Rolling on 24 h Cytokine- or Lipopolysaccharide-stimulated Bovine Endothelial Cells under Flow Conditions J. Exp. Med., November 17, 1997; 186(10): 1701 - 1711. [Abstract] [Full Text] [PDF]

				M. A. d. Pozo, C. Cabanas, M. C. Montoya, A. Ager, P. Sanchez-Mateos, and F. Sanchez-Madrid ICAMs Redistributed by Chemokines to Cellular Uropods as a Mechanism for Recruitment of T Lymphocytes J. Cell Biol., April 21, 1997; 137(2): 493 - 508. [Abstract] [Full Text] [PDF]