The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Silvennoinen, O.
Right arrow Articles by Campana, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Silvennoinen, O.
Right arrow Articles by Campana, D.

The Journal of Immunology, Vol 156, Issue 1 100-107, Copyright © 1996 by American Association of Immunologists

ARTICLES

CD38 signal transduction in human B cell precursors. Rapid induction of tyrosine phosphorylation, activation of syk tyrosine kinase, and phosphorylation of phospholipase C-gamma and phosphatidylinositol 3- kinase

O Silvennoinen, H Nishigaki, A Kitanaka, M Kumagai, C Ito, F Malavasi, Q Lin, ME Conley and D Campana
Department of Virology, University of Helsinki, Finland.

Ligation of CD38 inhibits proliferation and induces apoptosis of human immature B cells, but the molecular mechanisms underlying this function are unknown. We found that CD38 dimerization with the specific mAbs T16 and IB4 induces rapid and transient tyrosine phosphorylation of several intracellular proteins in the immature B cell lines RS4;11, REH, 380, Nalm6, and OP-1. This effect could be markedly reduced by incubating cells with the tyrosine kinase inhibitors genistein, staurosporine, and herbimycin A. CD38 dimerization induced tyrosine phosphorylation of the protein kinase syk and increased syk kinase activity. CD38 dimerization also induced tyrosine phosphorylation of phospholipase C-gamma and of the p85 subunit of phosphatidylinositol 3-kinase (PI 3-K). The latter was accompanied by a distinct increase in PI 3-kinase activity in the immunoprecipitates obtained with an anti-phosphotyrosine Ab. In contrast to the signaling triggered by surface Ig engagement in B lymphocytes, CD38 ligation did not appear to induce tyrosine phosphorylation of the src-like protein tyrosine kinases lyn, fyn, and btk, or of vav- and ras-GTPase-activating protein, nor did it induce detectable changes in cytosolic CA2+ concentrations. CD38 signaling also differed from cytokine-induced signaling in that it did not cause tyrosine phosphorylation of Jak1 and Jak2. Finally, CD38 ligation did not inhibit IL-3-induced tyrosine phosphorylation of Jak2. These results identify CD38 as a cell surface receptor with signal transduction properties activated by dimerization. Induction of signal transduction by CD38 ligation implies the existence of a yet unidentified natural ligand of CD38.


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
F. Malavasi, S. Deaglio, A. Funaro, E. Ferrero, A. L. Horenstein, E. Ortolan, T. Vaisitti, and S. Aydin
Evolution and Function of the ADP Ribosyl Cyclase/CD38 Gene Family in Physiology and Pathology
Physiol Rev, July 1, 2008; 88(3): 841 - 886.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Mayo, J. Jacob-Hirsch, N. Amariglio, G. Rechavi, M.-J. Moutin, F. E. Lund, and R. Stein
Dual Role of CD38 in Microglial Activation and Activation-Induced Cell Death
J. Immunol., July 1, 2008; 181(1): 92 - 103.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Deaglio, T. Vaisitti, S. Aydin, E. Ferrero, and F. Malavasi
In-tandem insight from basic science combined with clinical research: CD38 as both marker and key component of the pathogenetic network underlying chronic lymphocytic leukemia
Blood, August 15, 2006; 108(4): 1135 - 1144.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
F. E. Lund, H. Muller-Steffner, H. Romero-Ramirez, M. E. Moreno-Garcia, S. Partida-Sanchez, M. Makris, N. J. Oppenheimer, L. Santos-Argumedo, and F. Schuber
CD38 induces apoptosis of a murine pro-B leukemic cell line by a tyrosine kinase-dependent but ADP-ribosyl cyclase- and NAD glycohydrolase-independent mechanism
Int. Immunol., July 1, 2006; 18(7): 1029 - 1042.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M.-T. Zilber, N. Setterblad, T. Vasselon, C. Doliger, D. Charron, N. Mooney, and C. Gelin
MHC class II/CD38/CD9: a lipid-raft-dependent signaling complex in human monocytes
Blood, November 1, 2005; 106(9): 3074 - 3081.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
H. Kaku, K. Horikawa, Y. Obata, I. Kato, H. Okamoto, N. Sakaguchi, S. Gerondakis, and K. Takatsu
NF-{kappa}B is required for CD38-mediated induction of C{gamma}1 germline transcripts in murine B lymphocytes
Int. Immunol., September 1, 2002; 14(9): 1055 - 1064.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
R. Mallone, A. Funaro, M. Zubiaur, G. Baj, C. M. Ausiello, C. Tacchetti, J. Sancho, C. Grossi, and F. Malavasi
Signaling through CD38 induces NK cell activation
Int. Immunol., April 1, 2001; 13(4): 397 - 409.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
R. Mallone, E. Ortolan, G. Baj, A. Funaro, S. Giunti, E. Lillaz, F. Saccucci, M. Cassader, P. Cavallo-Perin, and F. Malavasi
Autoantibody Response to CD38 in Caucasian Patients With Type 1 and Type 2 Diabetes: Immunological and Genetic Characterization
Diabetes, April 1, 2001; 50(4): 752 - 762.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
C. F. Webb, Y. Yamashita, N. Ayers, S. Evetts, Y. Paulin, M. E. Conley, and E. A. Smith
The Transcription Factor Bright Associates with Bruton's Tyrosine Kinase, the Defective Protein in Immunodeficiency Disease
J. Immunol., December 15, 2000; 165(12): 6956 - 6965.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. J. Rebecchi and S. N. Pentyala
Structure, Function, and Control of Phosphoinositide-Specific Phospholipase C
Physiol Rev, October 1, 2000; 80(4): 1291 - 1335.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-S. Cho, M.-K. Han, Y. B. Choi, Y. Yun, J. Shin, and U.-H. Kim
Direct Interaction of the CD38 Cytoplasmic Tail and the Lck SH2 Domain. CD38 TRANSDUCES T CELL ACTIVATION SIGNALS THROUGH ASSOCIATED Lck
J. Biol. Chem., January 21, 2000; 275(3): 1685 - 1690.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
E. Todisco, T. Suzuki, K. Srivannaboon, E. Coustan-Smith, S. C. Raimondi, F. G. Behm, A. Kitanaka, and D. Campana
CD38 ligation inhibits normal and leukemic myelopoiesis
Blood, January 15, 2000; 95(2): 535 - 542.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. Sconocchia, J. A. Titus, A. Mazzoni, A. Visintin, F. Pericle, S. W. Hicks, F. Malavasi, and D. M. Segal
CD38 Triggers Cytotoxic Responses in Activated Human Natural Killer Cells
Blood, December 1, 1999; 94(11): 3864 - 3871.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Borrione, S. Peola, S. Mariani, B. Besostri, R. Mallone, F. Malavasi, A. Pileri, and M. Massaia
CD38 Stimulation Lowers the Activation Threshold and Enhances the Alloreactivity of Cord Blood T Cells by Activating the Phosphatidylinositol 3-Kinase Pathway and Inducing CD73 Expression
J. Immunol., May 15, 1999; 162(10): 6238 - 6246.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Kitanaka, T. Suzuki, C. Ito, H. Nishigaki, E. Coustan-Smith, T. Tanaka, Y. Kubota, and D. Campana
CD38-Mediated Signaling Events in Murine Pro-B Cells Expressing Human CD38 With or Without Its Cytoplasmic Domain
J. Immunol., February 15, 1999; 162(4): 1952 - 1958.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Konopleva, Z. Estrov, S. Zhao, M. Andreeff, and K. Mehta
Ligation of Cell Surface CD38 Protein with Agonistic Monoclonal Antibody Induces a Cell Growth Signal in Myeloid Leukemia Cells
J. Immunol., November 1, 1998; 161(9): 4702 - 4708.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Laser, V. S. Kasi, M. Hamawaki, G. Cooper IV, C. M. Kerr, and D. Kuppuswamy
Differential Activation of p70 and p85 S6 Kinase Isoforms during Cardiac Hypertrophy in the Adult Mammal
J. Biol. Chem., September 18, 1998; 273(38): 24610 - 24619.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. A. Cockayne, T. Muchamuel, J. C. Grimaldi, H. Muller-Steffner, T. D. Randall, F. E. Lund, R. Murray, F. Schuber, and M. C. Howard
Mice Deficient for the Ecto-Nicotinamide Adenine Dinucleotide Glycohydrolase CD38 Exhibit Altered Humoral Immune Responses
Blood, August 15, 1998; 92(4): 1324 - 1333.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. Morra, M. Zubiaur, C. Terhorst, J. Sancho, and F. Malavasi
CD38 is functionally dependent on the TCR/CD3 complex in human T cells
FASEB J, May 1, 1998; 12(7): 581 - 592.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
E. Zocchi, A. Daga, C. Usai, L. Franco, L. Guida, S. Bruzzone, A. Costa, C. Marchetti, and A. De Flora
Expression of CD38 Increases Intracellular Calcium Concentration and Reduces Doubling Time in HeLa and 3T3 Cells
J. Biol. Chem., April 3, 1998; 273(14): 8017 - 8024.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Venkataraman, N. Muthusamy, S. Muthukkumar, and S. Bondada
Activation of Lyn, Blk, and Btk But Not Syk in CD72-Stimulated B Lymphocytes
J. Immunol., April 1, 1998; 160(7): 3322 - 3329.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Funaro, M. Reinis, O. Trubiani, S. Santi, R. Di Primio, and F. Malavasi
CD38 Functions Are Regulated Through an Internalization Step
J. Immunol., March 1, 1998; 160(5): 2238 - 2247.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Kitanaka, H. Mano, M. Ellen Conley, and D. Campana
Expression and Activation of the Nonreceptor Tyrosine Kinase Tec in Human B Cells
Blood, February 1, 1998; 91(3): 940 - 948.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Deaglio, M. Morra, R. Mallone, C. M. Ausiello, E. Prager, G. Garbarino, U. Dianzani, H. Stockinger, and F. Malavasi
Human CD38 (ADP-Ribosyl Cyclase) Is a Counter-Receptor of CD31, an Ig Superfamily Member
J. Immunol., January 1, 1998; 160(1): 395 - 402.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Zubiaur, O. Fernandez, E. Ferrero, J. Salmeron, B. Malissen, F. Malavasi, and J. Sancho
CD38 Is Associated with Lipid Rafts and upon Receptor Stimulation Leads to Akt/Protein Kinase B and Erk Activation in the Absence of the CD3-zeta Immune Receptor Tyrosine-based Activation Motifs
J. Biol. Chem., January 4, 2002; 277(1): 13 - 22.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M.-T. Zilber, S. Gregory, R. Mallone, S. Deaglio, F. Malavasi, D. Charron, and C. Gelin
CD38 expressed on human monocytes: A coaccessory molecule in the superantigen-induced proliferation
PNAS, March 14, 2000; 97(6): 2840 - 2845.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.