| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 155, Issue 9 4476-4484, Copyright © 1995 by American Association of Immunologists
ARTICLES |
NY Yount, MS Wang, J Yuan, N Banaiee, AJ Ouellette and ME Selsted
Department of Pathology, College of Medicine, University of California, Irvine 92717, USA.
Defensins constitute a family of 3- to 4-kDa antimicrobial peptides that are stored in the cytoplasmic granules of neutrophils, some macrophages, and intestinal Paneth cells. We have assessed defensin gene expression during myeloid differentiation by first characterizing cDNAs for each of the four known rat neutrophil defensins (RatNP 1-4). The cDNA sequences revealed that the peptides are synthesized as 87-94 amino acid precursors, each containing signal, pro-, and mature peptide segments. RatNP-3 and -4 mRNAs, but not those for RatNP-1 and -2 or other myeloid defensins, contained unique polypurine tracts located close to the termination codon in the 3' untranslated region. By using cDNA probes and/or riboprobes, we evaluated defensin transcript levels in a variety of tissues and in the full spectrum of neutrophil precursors. By in situ hybridization, defensin mRNAs were localized to neutrophil precursors in the bone marrow, with the highest mRNA levels occurring in promyelocytes and somewhat lower signals occurring in myeloblasts and myelocytes. Defensin mRNAs were not detectable in bands or mature neutrophils, nor at significant levels in tissues other than bone marrow. The accumulation of defensin protein in bone marrow cells was assessed by immunohistochemical staining with anti-RatNP-1 Ab. RatNP 1-4 mRNAs and protein levels were then correlated for each stage of neutrophilic differentiation to reveal the maturational profile of myeloid defensin gene expression in the rat.
This article has been cited by other articles:
|
|
 |
|
  C. Pierrot, E. Adam, D. Hot, S. Lafitte, M. Capron, J. D. George, and J. Khalife Contribution of T Cells and Neutrophils in Protection of Young Susceptible Rats from Fatal Experimental Malaria J. Immunol., February 1, 2007; 178(3): 1713 - 1722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Porter, H. Yang, S. Yavagal, G. C. Preza, O. Murillo, H. Lima, S. Greene, L. Mahoozi, M. Klein-Patel, G. Diamond, et al. Distinct Defensin Profiles in Neisseria gonorrhoeae and Chlamydia trachomatis Urethritis Reveal Novel Epithelial Cell-Neutrophil Interactions Infect. Immun., August 1, 2005; 73(8): 4823 - 4833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M.R. Aldred, E. J. Hollox, and J. A.L. Armour Copy number polymorphism and expression level variation of the human {alpha}-defensin genes DEFA1 and DEFA3 Hum. Mol. Genet., July 15, 2005; 14(14): 2045 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Patil, A. L. Hughes, and G. Zhang Rapid evolution and diversification of mammalian {alpha}-defensins as revealed by comparative analysis of rodent and primate genes Physiol Genomics, December 15, 2004; 20(1): 1 - 11. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Fujiwara, M. Ochiai, T. Ohta, M. Ohki, H. Aburatani, M. Nagao, T. Sugimura, and H. Nakagama Global gene expression analysis of rat colon cancers induced by a food-borne carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine Carcinogenesis, August 1, 2004; 25(8): 1495 - 1505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tanabe, J. Yuan, M. M. Zaragoza, S. Dandekar, A. Henschen-Edman, M. E. Selsted, and A. J. Ouellette Paneth Cell {alpha}-Defensins from Rhesus Macaque Small Intestine Infect. Immun., March 1, 2004; 72(3): 1470 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Yamamoto, N. Banaiee, N. Y. Yount, B. Patel, and M. E. Selsted {alpha}-Defensin expression during myelopoiesis: identification of cis and trans elements that regulate expression of NP-3 in rat promyelocytes J. Leukoc. Biol., February 1, 2004; 75(2): 332 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. T. Lay, F. Brugliera, and M. A. Anderson Isolation and Properties of Floral Defensins from Ornamental Tobacco and Petunia Plant Physiology, March 1, 2003; 131(3): 1283 - 1293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Com, F. Bourgeon, B. Evrard, T. Ganz, D. Colleu, B. Jegou, and C. Pineau Expression of Antimicrobial Defensins in the Male Reproductive Tract of Rats, Mice, and Humans Biol Reprod, January 1, 2003; 68(1): 95 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Tydell, N. Yount, D. Tran, J. Yuan, and M. E. Selsted Isolation, Characterization, and Antimicrobial Properties of Bovine Oligosaccharide-binding Protein. A MICROBICIDAL GRANULE PROTEIN OF EOSINOPHILS AND NEUTROPHILS J. Biol. Chem., May 24, 2002; 277(22): 19658 - 19664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Kaiser and G. Diamond Expression of mammalian defensin genes J. Leukoc. Biol., December 1, 2000; 68(6): 779 - 784. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-Q. Tang, J. Yuan, C. J. Miller, and M. E. Selsted Isolation, Characterization, cDNA Cloning, and Antimicrobial Properties of Two Distinct Subfamilies of alpha -Defensins from Rhesus Macaque Leukocytes Infect. Immun., November 1, 1999; 67(11): 6139 - 6144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tang, J. Yuan, G. Ösapay, K. Ösapay, D. Tran, C. J. Miller, A. J. Ouellette, and M. E. Selsted A Cyclic Antimicrobial Peptide Produced in Primate Leukocytes by the Ligation of Two Truncated -Defensins Science, October 15, 1999; 286(5439): 498 - 502. [Abstract] [Full Text]
|
|
|
|
|
|
N. Y. Yount, J. Yuan, A. Tarver, T. Castro, G. Diamond, P. A. Tran, J. N. Levy, C. McCullough, J. S. Cullor, C. L. Bevins, et al. Cloning and Expression of Bovine Neutrophil beta -Defensins. BIOSYNTHETIC PROFILE DURING NEUTROPHILIC MATURATION AND LOCALIZATION OF MATURE PEPTIDE TO NOVEL CYTOPLASMIC DENSE GRANULES J. Biol. Chem., September 10, 1999; 274(37): 26249 - 26258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. JEAN, S. REZAIGUIA-DELCLAUX, C. DELACOURT, R. LECLERCQ, C. LAFUMA, C. BRUN-BUISSON, A. HARF, and C. DELCLAUX Protective Effect of Endotoxin Instillation on Subsequent Bacteria-induced Acute Lung Injury in Rats Am. J. Respir. Crit. Care Med., December 1, 1998; 158(6): 1702 - 1708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Westendorf, C. M. Yamamoto, N. Lenny, J. R. Downing, M. E. Selsted, and S. W. Hiebert The t(8;21) Fusion Product, AML-1-ETO, Associates with C/EBP-alpha , Inhibits C/EBP-alpha -Dependent Transcription, and Blocks Granulocytic Differentiation Mol. Cell. Biol., January 1, 1998; 18(1): 322 - 333. [Abstract] [Full Text]
|
|
|
|
|
|
R. L. Gallo, K. J. Kim, M. Bernfield, C. A. Kozak, M. Zanetti, L. Merluzzi, and R. Gennaro Identification of CRAMP, a Cathelin-related Antimicrobial Peptide Expressed in the Embryonic and Adult Mouse J. Biol. Chem., May 16, 1997; 272(20): 13088 - 13093. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
