The Journal of Immunology, Vol 155, Issue 8 4072-4077, Copyright © 1995 by American Association of Immunologists

ARTICLES

T lymphocytes from volunteers immunized with irradiated Plasmodium falciparum sporozoites recognize liver and blood stage malaria antigens

U Krzych, JA Lyon, T Jareed, I Schneider, MR Hollingdale, DM Gordon and WR Ballou
Department of Immunology, Walter Reed Army Institute of Research, Washington, DC 20307, USA.

The model of protective immunity induced by immunization with irradiated plasmodia sporozoites (SPZ) has become the prototype for a promising vaccine strategy based on Ab and CTL responses directed against pre-erythrocytic stage Ags, in particular the circumsporozoite protein (CSP) and sporozoite surface protein 2 (SSP2). However, results from recently conducted vaccine studies suggest that T cell responses directed against additional specificities might also be required for protection. We have tested this hypothesis by examining human T lymphocytes from irradiated Plasmodium falciparum SPZ-immune volunteers for proliferative reactivities to parasitized red blood cells (pRBC) and recombinant proteins and synthetic peptides representing certain liver and blood stage Ags. In this work, we report that although SPZ- induced protective immunity is stage-specific, SPZ-immune lymphocytes recognized determinants associated with erythrocytic and liver stage parasites. Thus, protective immunity induced by irradiated SPZ may depend upon responses against pre-erythrocytic Ags in addition to CSP and SSP2.

This article has been cited by other articles:

				S. Pichyangkul, U. Kum-Arb, K. Yongvanitchit, A. Limsalakpetch, M. Gettayacamin, D. E. Lanar, L. A. Ware, V. A. Stewart, D. G. Heppner, P. Mettens, et al. Preclinical Evaluation of the Safety and Immunogenicity of a Vaccine Consisting of Plasmodium falciparum Liver-Stage Antigen 1 with Adjuvant AS01B Administered Alone or Concurrently with the RTS,S/AS01B Vaccine in Rhesus Primates Infect. Immun., January 1, 2008; 76(1): 229 - 238. [Abstract] [Full Text] [PDF]

				C. Brando, L. A. Ware, H. Freyberger, A. Kathcart, A. Barbosa, S. Cayphas, M.-A. Demoitie, P. Mettens, D. G. Heppner, and D. E. Lanar Murine Immune Responses to Liver-Stage Antigen 1 Protein FMP011, a Malaria Vaccine Candidate, Delivered with Adjuvant AS01B or AS02A Infect. Immun., February 1, 2007; 75(2): 838 - 845. [Abstract] [Full Text] [PDF]

				J. E. Garcia, A. Puentes, and M. E. Patarroyo Developmental Biology of Sporozoite-Host Interactions in Plasmodium falciparum Malaria: Implications for Vaccine Design Clin. Microbiol. Rev., October 1, 2006; 19(4): 686 - 707. [Abstract] [Full Text] [PDF]

				U. Woehlbier, C. Epp, C. W. Kauth, R. Lutz, C. A. Long, B. Coulibaly, B. Kouyate, M. Arevalo-Herrera, S. Herrera, and H. Bujard Analysis of Antibodies Directed against Merozoite Surface Protein 1 of the Human Malaria Parasite Plasmodium falciparum Infect. Immun., February 1, 2006; 74(2): 1313 - 1322. [Abstract] [Full Text] [PDF]

				C. J. Hillier, L. A. Ware, A. Barbosa, E. Angov, J. A. Lyon, D. G. Heppner, and D. E. Lanar Process Development and Analysis of Liver-Stage Antigen 1, a Preerythrocyte-Stage Protein-Based Vaccine for Plasmodium falciparum Infect. Immun., April 1, 2005; 73(4): 2109 - 2115. [Abstract] [Full Text] [PDF]

				T. C. Luke and S. L. Hoffman Rationale and plans for developing a non-replicating, metabolically active, radiation-attenuated Plasmodium falciparum sporozoite vaccine J. Exp. Biol., November 1, 2003; 206(21): 3803 - 3808. [Abstract] [Full Text] [PDF]

				C. C. John, P. O. Sumba, J. H. Ouma, B. L. Nahlen, C. L. King, and J. W. Kazura Cytokine Responses to Plasmodium falciparum Liver-Stage Antigen 1 Vary in Rainy and Dry Seasons in Highland Kenya Infect. Immun., September 1, 2000; 68(9): 5198 - 5204. [Abstract] [Full Text] [PDF]

				S. K. Joshi, A. Bharadwaj, S. Chatterjee, and V. S. Chauhan Analysis of Immune Responses against T- and B-Cell Epitopes from Plasmodium falciparum Liver-Stage Antigen 1 in Rodent Malaria Models and Malaria-Exposed Human Subjects in India Infect. Immun., January 1, 2000; 68(1): 141 - 150. [Abstract] [Full Text] [PDF]