The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wen, R.
Right arrow Articles by Woodland, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wen, R.
Right arrow Articles by Woodland, D. L.

The Journal of Immunology, Vol 155, Issue 4 1884-1892, Copyright © 1995 by American Association of Immunologists

ARTICLES

Variable influence of MHC polymorphism on the recognition of bacterial superantigens by T cells

R Wen, MA Blackman and DL Woodland
Department of Pathology, University of Tennessee, Memphis 38163, USA.

Superantigen recognition by some T cells is strongly influenced by polymorphic residues of the MHC class II molecule, suggesting that the TCR contacts the class II molecule during superantigen engagement. However, the degree of MHC preference varies with V beta expression. For example, the recognition of staphylococcal enterotoxin B (SEB) by murine V beta 14+ T cell hybridomas is strongly influenced by MHC polymorphism, whereas the recognition of SEB by V beta 8.2+ hybridomas appears to be independent of MHC polymorphism. One possible explanation for this difference is that V beta 14+ TCR may have a lower avidity for SEB such that the stability of the TCR/SEB/MHC complex is more strongly influenced by potential TCR/MHC interactions. To investigate this possibility, we examined the MHC preference of SEB recognition by murine V beta 8.2+/CD4+ T cell hybridomas in which we altered the strength of SEB recognition. First, we compared the recognition of wild- type SEB and mutant SEB, which only weakly activates T cells. Second, we compared recognition of SEB presented by I-E and I-A molecules, which differ in their affinity for SEB. In both cases, the degree of MHC preference was significantly increased when the strength of SEB recognition was reduced. Furthermore, we used mutant MHC class II molecules to show that the degree of MHC preference was controlled by residues of the class II molecule predicted to interact with the TCR during SEB recognition. Taken together, these data support the idea that there is a TCR/MHC interaction during SEB recognition, and the influence of this interaction varies with the overall avidity of the TCR/SEB/MHC complex.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
M. Llewelyn, S. Sriskandan, M. Peakman, D. R. Ambrozak, D. C. Douek, W. W. Kwok, J. Cohen, and D. M. Altmann
HLA Class II Polymorphisms Determine Responses to Bacterial Superantigens
J. Immunol., February 1, 2004; 172(3): 1719 - 1726.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
C. Noel, S. Florquin, M. Goldman, and M. Y. Braun
Chronic exposure to superantigen induces regulatory CD4+ T cells with IL-10-mediated suppressive activity
Int. Immunol., April 1, 2001; 13(4): 431 - 439.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
P.-L. Li, R. E. Tiedemann, S. L. Moffat, and J. D. Fraser
The Superantigen Streptococcal Pyrogenic Exotoxin C (SPE-C) Exhibits a Novel Mode of Action
J. Exp. Med., August 4, 1997; 186(3): 375 - 383.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.