| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 155, Issue 4 1862-1872, Copyright © 1995 by American Association of Immunologists
ARTICLES |
D Vanhecke, G Leclercq, J Plum and B Vandekerckhove
Department of Clinical Biology, Microbiology and Immunology, University Hospital, Ghent, Belgium.
During thymocyte development, CD69 expression is induced at an early stage of positive selection. To examine the differentiation of human CD69+CD3+ thymocytes, discrete phenotypes were defined by the relative expression of CD69, CD27, CD1, and CD45RA/RO: CD3+CD69- thymocytes were CD27-CD1+CD45RA-/RO+ (phenotype I), whereas the CD3+ CD69+ population could be subdivided into CD27-CD1+CD45RA-/RA+ (phenotype II), CD27+CD1+CD45RA-/RO+ (phenotype III), CD27+CD1-CD45RA-/dull/RO+ (phenotype IV), and CD27+CD1-CD45RA+/ROdull (phenotype V) thymocytes. Phenotype I thymocytes were CD4+CD8 alpha beta + double positive (DP). Phenotype II thymocytes contained DP and CD4+CD8 alpha dullCD8 beta- cells, whereas phenotype III thymocytes were DP and mostly CD4dullCD8 alpha beta+, indicating that CD27 on DP cells may be a marker for CD8- committed cells. Results obtained with SCID-hu mice, transplanted with human fetal thymus and liver, showed that immature human CD69-CD3+ thymocytes were corticosteroid-sensitive, whereas essentially all CD69+CD3+ cells were resistant. During differentiation of one cohort of corticosteroid-resistant CD69+CD3+ thymocytes, phenotype II and III thymocytes, disappeared within a week, whereas the percentage of phenotype IV and especially V thymocytes increased, suggesting that the latter represent the end stages in differentiation. Recent thymic emigrants in SCID-hu mice were identified as CD69-CD27+CD1-CD45RA- /dull/RO+ or CD45RA+/ROdull cells. Because phenotype IV and phenotype V thymocytes rapidly lose CD69 expression in cell culture, these thymocytes are probably the cells that are exported from the thymus. In conclusion, after the acquisition of CD69, thymocyte differentiation appears to continue in an ordered pattern, and cells that eventually leave the thymus are CD69-CD1-CD45RA+ or CD45RA-/dull.
This article has been cited by other articles:
|
|
 |
|
  M. Sinkora, J. Sinkorova, Z. Cimburek, and W. Holtmeier Two Groups of Porcine TCR{gamma}{delta}+ Thymocytes Behave and Diverge Differently J. Immunol., January 15, 2007; 178(2): 711 - 719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Seddiki, B. Santner-Nanan, S. G. Tangye, S. I. Alexander, M. Solomon, S. Lee, R. Nanan, and B. F. de Saint Groth Persistence of naive CD45RA+ regulatory T cells in adult life Blood, April 1, 2006; 107(7): 2830 - 2838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Legrand, K. Weijer, and H. Spits Experimental Models to Study Development and Function of the Human Immune System In Vivo J. Immunol., February 15, 2006; 176(4): 2053 - 2058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gutierrez-Frias, R. Sacedon, C. Hernandez-Lopez, T. Cejalvo, T. Crompton, A. G. Zapata, A. Varas, and A. Vicente Sonic Hedgehog Regulates Early Human Thymocyte Differentiation by Counteracting the IL-7-Induced Development of CD34+ Precursor Cells J. Immunol., October 15, 2004; 173(8): 5046 - 5053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Strobel, M. Helmreich, G. Menioudakis, S. R. Lewin, T. Rudiger, A. Bauer, V. Hoffacker, R. Gold, W. Nix, B. Schalke, et al. Paraneoplastic myasthenia gravis correlates with generation of mature naive CD4+ T cells in thymomas Blood, June 17, 2002; 100(1): 159 - 166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. C. C. Kerre, G. De Smet, M. De Smedt, A. Zippelius, M. J. Pittet, A. W. Langerak, J. De Bosscher, F. Offner, B. Vandekerckhove, and J. Plum Adapted NOD/SCID model supports development of phenotypically and functionally mature T cells from human umbilical cord blood CD34+ cells Blood, March 1, 2002; 99(5): 1620 - 1626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hernandez-Lopez, A. Varas, R. Sacedon, E. Jimenez, J. J. Munoz, A. G. Zapata, and A. Vicente Stromal cell-derived factor 1/CXCR4 signaling is critical for early human T-cell development Blood, January 15, 2002; 99(2): 546 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakayama, D. J. Kasprowicz, M. Yamashita, L. A. Schubert, G. Gillard, M. Kimura, A. Didierlaurent, H. Koseki, and S. F. Ziegler The Generation of Mature, Single-Positive Thymocytes In Vivo Is Dysregulated by CD69 Blockade or Overexpression J. Immunol., January 1, 2002; 168(1): 87 - 94. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Imlach, S. McBreen, T. Shirafuji, C. Leen, J. E. Bell, and P. Simmonds Activated Peripheral CD8 Lymphocytes Express CD4 In Vivo and Are Targets for Infection by Human Immunodeficiency Virus Type 1 J. Virol., December 1, 2001; 75(23): 11555 - 11564. [Abstract] [Full Text]
|
|
|
|
|
|
T. Taghon, M. De Smedt, F. Stolz, M. Cnockaert, J. Plum, and G. Leclercq Enforced Expression of GATA-3 Severely Reduces Human Thymic Cellularity J. Immunol., October 15, 2001; 167(8): 4468 - 4475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Martin, X. Romero, M. A. de la Fuente, V. Tovar, N. Zapater, E. Esplugues, P. Pizcueta, J. Bosch, and P. Engel CD84 Functions as a Homophilic Adhesion Molecule and Enhances IFN-{gamma} Secretion: Adhesion Is Mediated by Ig-Like Domain 1 J. Immunol., October 1, 2001; 167(7): 3668 - 3676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Guillemard, M.-T. Nugeyre, L. Chene, N. Schmitt, C. Jacquemot, F. Barre-Sinoussi, and N. Israel Interleukin-7 and infection itself by human immunodeficiency virus 1 favor virus persistence in mature CD4+CD8{-}CD3+ thymocytes through sustained induction of Bcl-2 Blood, October 1, 2001; 98(7): 2166 - 2174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. de la Fuente, V. Tovar, N. Villamor, N. Zapater, P. Pizcueta, E. Campo, J. Bosch, and P. Engel Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9 Blood, June 1, 2001; 97(11): 3513 - 3520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Hoffacker, A. Schultz, J. J. Tiesinga, R. Gold, B. Schalke, W. Nix, R. Kiefer, H. K. Muller-Hermelink, and A. Marx Thymomas alter the T-cell subset composition in the blood: a potential mechanism for thymoma-associated autoimmune disease Blood, December 1, 2000; 96(12): 3872 - 3879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Varas, E. Jimenez, R. Sacedon, M. Rodriguez-Mahou, E. Maroto, A. G. Zapata, and A. Vicente Analysis of the Human Neonatal Thymus: Evidence for a Transient Thymic Involution J. Immunol., June 15, 2000; 164(12): 6260 - 6267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Verhasselt, E. Naessens, C. Verhofstede, M. De Smedt, S. Schollen, T. Kerre, D. Vanhecke, and J. Plum Human Immunodeficiency Virus nef Gene Expression Affects Generation and Function of Human T Cells, But Not Dendritic Cells Blood, October 15, 1999; 94(8): 2809 - 2818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chêne, M.-T. Nugeyre, E. Guillemard, N. Moulian, F. Barré-Sinoussi, and N. Israël Thymocyte-Thymic Epithelial Cell Interaction Leads to High-Level Replication of Human Immunodeficiency Virus Exclusively in Mature CD4+ CD8- CD3+ Thymocytes: a Critical Role for Tumor Necrosis Factor and Interleukin-7 J. Virol., September 1, 1999; 73(9): 7533 - 7542. [Abstract] [Full Text]
|
|
|
|
|
|
B. Blom, M. C.M. Verschuren, M. H.M. Heemskerk, A. Q. Bakker, E. J. van Gastel-Mol, I. L.M. Wolvers-Tettero, J. J.M. van Dongen, and H. Spits TCR Gene Rearrangements and Expression of the Pre-T Cell Receptor Complex During Human T-Cell Differentiation Blood, May 1, 1999; 93(9): 3033 - 3043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Plum, M. De Smedt, B. Verhasselt, F. Offner, T. Kerre, D. Vanhecke, G. Leclercq, and B. Vandekerckhove In Vitro Intrathymic Differentiation Kinetics of Human Fetal Liver CD34+CD38- Progenitors Reveals a Phenotypically Defined Dendritic/T-NK Precursor Split J. Immunol., January 1, 1999; 162(1): 60 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pedroza-Martins, K. B. Gurney, B. E. Torbett, and C. H. Uittenbogaart Differential Tropism and Replication Kinetics of Human Immunodeficiency Virus Type 1 Isolates in Thymocytes: Coreceptor Expression Allows Viral Entry, but Productive Infection of Distinct Subsets Is Determined at the Postentry Level J. Virol., December 1, 1998; 72(12): 9441 - 9452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hacein-Bey, G. D. S. Basile, J. Lemerle, A. Fischer, and M. Cavazzana-Calvo gamma c Gene Transfer in the Presence of Stem Cell Factor, FLT-3L, Interleukin-7 (IL-7), IL-1alpha , and IL-15 Cytokines Restores T-Cell Differentiation From gamma c(-) X-Linked Severe Combined Immunodeficiency Hematopoietic Progenitor Cells in Murine Fetal Thymic Organ Cultures Blood, December 1, 1998; 92(11): 4090 - 4097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Krishna and A. Varki 9-O-Acetylation of Sialomucins: A Novel Marker of Murine CD4 T Cells that Is Regulated during Maturation and Activation J. Exp. Med., June 2, 1997; 185(11): 1997 - 2013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sharfe, H. K. Dadi, M. Shahar, and C. M. Roifman Human immune disorder arising from mutation of the alpha chain of the interleukin-2 receptor PNAS, April 1, 1997; 94(7): 3168 - 3171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Res, B. Blom, T. Hori, K. Weijer, and H. Spits Downregulation of CD1 Marks Acquisition of Functional Maturation of Human Thymocytes and Defines a Control Point in Late Stages of Human T Cell Development J. Exp. Med., January 1, 1997; 185(1): 141 - 152. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
