| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 155, Issue 3 1252-1263, Copyright © 1995 by American Association of Immunologists
ARTICLES |
R Pardi, G Bossi, L Inverardi, E Rovida and JR Bender
Scientific Institute San Raffaele-DIBIT, University of Milano School of Medicine, Italy.
Selected functions of integrins, including regulated cytoskeletal association and transmembrane signaling, depend on a poorly defined bidirectional communication between the extracellular and cytoplasmic domains of the alpha and beta subunits. To investigate this problem in the leukocyte integrin alpha L beta 2 (LFA-1), we generated a series of cytoplasmic truncation or internal substitution mutants of the alpha L and beta 2 cytoplasmic domains, and assessed their biochemical and functional properties upon ectopic expression in constitutively adherent cells. Expression of the alpha L beta 2 heterodimer in stably adherent cells is sufficient to promote its constitutive cytoskeletal association. Structural determinants for such association are located in selected regions of the beta 2 cytoplasmic domain, which display functional interdependence. In addition, a conserved region (Arg733- Lys742) in the beta 2 cytoplasmic domain seems to be critical not only for its cytoskeletal association, but also for endoplasmic reticulum retention, assembly, and transport to the plasma membrane of the mature alpha L beta 2 heterodimer. Analysis of deletion mutants of the alpha L subunit demonstrates a role of the conserved, membrane-proximal GFFKR motif in conferring stability to the alpha beta complex, possibly because of its direct involvement in heterodimer formation. We propose that a previously uncharacterized association of defined subregions of the cytoplasmic domains of integrin alpha and beta subunits affects the dimerization and regulated function of the adhesion receptor.
This article has been cited by other articles:
|
|
 |
|
  W. M. McKillop, J. W. Barrett, S. H. Pasternak, B. M. C. Chan, and G. A. Dekaban The extracellular domain of CD11d regulates its cell surface expression J. Leukoc. Biol., October 1, 2009; 86(4): 851 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Van de Walle, K. Vanhoorelbeke, Z. Majer, E. Illyes, J. Baert, I. Pareyn, and H. Deckmyn Two Functional Active Conformations of the Integrin {alpha}2{beta}1, Depending on Activation Condition and Cell Type J. Biol. Chem., November 4, 2005; 280(44): 36873 - 36882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Kanse, R. L. Matz, K. T. Preissner, and K. Peter Promotion of Leukocyte Adhesion by a Novel Interaction Between Vitronectin and the {beta}2 Integrin Mac-1 ({alpha}M{beta}2, CD11b/CD18) Arterioscler Thromb Vasc Biol, December 1, 2004; 24(12): 2251 - 2256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Wang, T. M. Leisner, and L. V. Parise Platelet {alpha}2{beta}1 integrin activation: contribution of ligand internalization and the {alpha}2-cytoplasmic domain Blood, August 15, 2003; 102(4): 1307 - 1315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang, Z. Li, E.-K. Viklund, and S. Stromblad p21-activated kinase 4 interacts with integrin {alpha}v{beta}5 and regulates {alpha}v{beta}5-mediated cell migration J. Cell Biol., September 29, 2002; 158(7): 1287 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Abraham and J. Miller Molecular Mechanisms of IL-2 Gene Regulation Following Costimulation Through LFA-1 J. Immunol., November 1, 2001; 167(9): 5193 - 5201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sancho, A. G. Santis, J. L. Alonso-Lebrero, F. Viedma, R. Tejedor, and F. Sanchez-Madrid Functional Analysis of Ligand-Binding and Signal Transduction Domains of CD69 and CD23 C-Type Lectin Leukocyte Receptors J. Immunol., October 1, 2000; 165(7): 3868 - 3875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-M. Tan, R. H. Hyland, A. Al-Shamkhani, W. A. Douglass, J. M. Shaw, and S. K. A. Law Effect of Integrin {beta}2 Subunit Truncations on LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) Assembly, Surface Expression, and Function J. Immunol., September 1, 2000; 165(5): 2574 - 2581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shenkman, M. Ehrlich, and G. Z. Lederkremer Masking of an Endoplasmic Reticulum Retention Signal by Its Presence in the Two Subunits of the Asialoglycoprotein Receptor J. Biol. Chem., January 28, 2000; 275(4): 2845 - 2851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V Martel, L Vignoud, S Dupe, P Frachet, M. Block, and C Albiges-Rizo Talin controls the exit of the integrin alpha 5 beta 1 from an early compartment of the secretory pathway J. Cell Sci., January 6, 2000; 113(11): 1951 - 1961. [Abstract] [PDF]
|
|
|
|
|
|
A. Armulik, I. Nilsson, G. von Heijne, and S. Johansson Determination of the Border between the Transmembrane and Cytoplasmic Domains of Human Integrin Subunits J. Biol. Chem., December 24, 1999; 274(52): 37030 - 37034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. van Kooyk, S. J. van Vliet, and C. G. Figdor The Actin Cytoskeleton Regulates LFA-1 Ligand Binding through Avidity Rather than Affinity Changes J. Biol. Chem., September 17, 1999; 274(38): 26869 - 26877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Abraham, J. Griffith, and J. Miller The Dependence for Leukocyte Function-Associated Antigen-1/ICAM-1 Interactions in T Cell Activation Cannot Be Overcome by Expression of High Density TCR Ligand J. Immunol., April 15, 1999; 162(8): 4399 - 4405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. PETTIT Cytosolic Free Calcium and the Cytoskeleton in the Control of Leukocyte Chemotaxis Physiol Rev, October 1, 1998; 78(4): 949 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Zuckerman, L. Pullen, and J. Miller Functional Consequences of Costimulation by ICAM-1 on IL-2 Gene Expression and T Cell Activation J. Immunol., April 1, 1998; 160(7): 3259 - 3268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Bleijs, G. C. F. van Duijnhoven, S. J. van Vliet, J. P. H. Thijssen, C. G. Figdor, and Y. van Kooyk A Single Amino Acid in the Cytoplasmic Domain of the beta 2 Integrin Lymphocyte Function-associated Antigen-1 Regulates Avidity-dependent Inside-out Signaling J. Biol. Chem., March 23, 2001; 276(13): 10338 - 10346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. F. Coe, J. A. Askari, A. D. Kline, M. K. Robinson, H. Kirby, P. E. Stephens, and M. J. Humphries Generation of a Minimal alpha 5beta 1 Integrin-Fc Fragment J. Biol. Chem., September 14, 2001; 276(38): 35854 - 35866. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
