| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 155, Issue 3 1229-1239, Copyright © 1995 by American Association of Immunologists
ARTICLES |
T Imai, M Kakizaki, M Nishimura and O Yoshie
Shionogi Institute for Medical Science, Osaka, Japan.
Previously, we have shown that CD81 and CD82, two members of the transmembrane 4 superfamily, form multimolecular membrane complexes by associating with each other and with CD4 or CD8 in T cells. In the present study, we further analyzed the molecular basis of the CD4 association with CD81 and CD82 by co-precipitation experiments. First, we examined the regions of CD4 involved in the association with CD81 and CD82 by employing chimeric proteins generated from CD4 and CD2. It was confirmed that CD4, but not CD2, was capable of binding with CD81 and CD82 in transfected cells. We found that the cytoplasmic region of CD4 was sufficient for the chimeric proteins to co-precipitate CD81, while both the cytoplasmic and extracellular regions of CD4 were required for them to efficiently co-precipitate CD82. We next found, by using truncated CD4 lacking the C-terminal 31 amino acids or mutated CD4 with the cysteine residues at 394 and 397 replaced by serine, that the p56lck binding site or the covalent modification with palmitic acid was not necessary for CD4 to associate with CD81 and CD82. Finally, we found that the binding of p56lck to CD4 strongly inhibited its association with CD81 and CD82. It is, therefore, suggested that CD4 exists at least in two physical states, one associated with p56lck and another associated with CD81 and CD82 in the absence or uncoupling of p56lck.
This article has been cited by other articles:
|
|
 |
|
  T. Lekishvili, E. Fromm, M. Mujoomdar, and F. Berditchevski The tumour-associated antigen L6 (L6-Ag) is recruited to the tetraspanin-enriched microdomains: implication for tumour cell motility J. Cell Sci., March 1, 2008; 121(5): 685 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gordon-Alonso, M. Yanez-Mo, O. Barreiro, S. Alvarez, M. A. Munoz-Fernandez, A. Valenzuela-Fernandez, and F. Sanchez-Madrid Tetraspanins CD9 and CD81 Modulate HIV-1-Induced Membrane Fusion J. Immunol., October 15, 2006; 177(8): 5129 - 5137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-H. Ho, F. Martin, A. Higginbottom, L. J. Partridge, V. Parthasarathy, G. W. Moseley, P. Lopez, C. Cheng-Mayer, and P. N. Monk Recombinant extracellular domains of tetraspanin proteins are potent inhibitors of the infection of macrophages by human immunodeficiency virus type 1. J. Virol., July 1, 2006; 80(13): 6487 - 6496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Seigneuret Complete Predicted Three-Dimensional Structure of the Facilitator Transmembrane Protein and Hepatitis C Virus Receptor CD81: Conserved and Variable Structural Domains in the Tetraspanin Superfamily Biophys. J., January 1, 2006; 90(1): 212 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Tardif and M. J. Tremblay Tetraspanin CD81 Provides a Costimulatory Signal Resulting in Increased Human Immunodeficiency Virus Type 1 Gene Expression in Primary CD4+ T Lymphocytes through NF-{kappa}B, NFAT, and AP-1 Transduction Pathways J. Virol., April 1, 2005; 79(7): 4316 - 4328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Furuya, H. Kato, N. Nishimura, I. Ishiwata, H. Ikeda, R. Ito, T. Yoshiki, and H. Ishikura Down-regulation of CD9 in Human Ovarian Carcinoma Cell Might Contribute to Peritoneal Dissemination: Morphologic Alteration and Reduced Expression of {beta}1 Integrin Subsets Cancer Res., April 1, 2005; 65(7): 2617 - 2625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Fallas, H. M. Tobin, O. Lou, D. Guo, D. B. Sant'Angelo, and L. K. Denzin Ectopic Expression of HLA-DO in Mouse Dendritic Cells Diminishes MHC Class II Antigen Presentation J. Immunol., August 1, 2004; 173(3): 1549 - 1560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. van Spriel, K. L. Puls, M. Sofi, D. Pouniotis, H. Hochrein, Z. Orinska, K.-P. Knobeloch, M. Plebanski, and M. D. Wright A Regulatory Role for CD37 in T Cell Proliferation J. Immunol., March 1, 2004; 172(5): 2953 - 2961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mittelbrunn, M. Yanez-Mo, D. Sancho, A. Ursa, and F. Sanchez-Madrid Cutting Edge: Dynamic Redistribution of Tetraspanin CD81 at the Central Zone of the Immune Synapse in Both T Lymphocytes and APC J. Immunol., December 15, 2002; 169(12): 6691 - 6695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Deng, R. H. Dekruyff, G. J. Freeman, D. T. Umetsu, and S. Levy Critical role of CD81 in cognate T-B cell interactions leading to Th2 responses Int. Immunol., May 1, 2002; 14(5): 513 - 523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yang, C. Claas, S.-K. Kraeft, L. B. Chen, Z. Wang, J. A. Kreidberg, and M. E. Hemler Palmitoylation of Tetraspanin Proteins: Modulation of CD151 Lateral Interactions, Subcellular Distribution, and Integrin-dependent Cell Morphology Mol. Biol. Cell, March 1, 2002; 13(3): 767 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Delaguillaumie, C. Lagaudriere-Gesbert, M. R. Popoff, and H. Conjeaud Rho GTPases link cytoskeletal rearrangements and activation processes induced via the tetraspanin CD82 in T lymphocytes J. Cell Sci., January 15, 2002; 115(2): 433 - 443. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Seigneuret, A. Delaguillaumie, C. Lagaudriere-Gesbert, and H. Conjeaud Structure of the Tetraspanin Main Extracellular Domain. A PARTIALLY CONSERVED FOLD WITH A STRUCTURALLY VARIABLE DOMAIN INSERTION J. Biol. Chem., October 19, 2001; 276(43): 40055 - 40064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Deng, V. P. Yeung, D. Tsitoura, R. H. DeKruyff, D. T. Umetsu, and S. Levy Allergen-Induced Airway Hyperreactivity Is Diminished in CD81-Deficient Mice J. Immunol., November 1, 2000; 165(9): 5054 - 5061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Witherden, R. Boismenu, and W. L. Havran CD81 and CD28 Costimulate T Cells Through Distinct Pathways J. Immunol., August 15, 2000; 165(4): 1902 - 1909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Denzer, M. van Eijk, M. J. Kleijmeer, E. Jakobson, C. de Groot, and H. J. Geuze Follicular Dendritic Cells Carry MHC Class II-Expressing Microvesicles at Their Surface J. Immunol., August 1, 2000; 165(3): 1259 - 1265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Yauch, A. R. Kazarov, B. Desai, R. T. Lee, and M. E. Hemler Direct Extracellular Contact between Integrin alpha 3beta 1 and TM4SF Protein CD151 J. Biol. Chem., March 24, 2000; 275(13): 9230 - 9238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Denzer, M. Kleijmeer, H. Heijnen, W Stoorvogel, and H. Geuze Exosome: from internal vesicle of the multivesicular body to intercellular signaling device J. Cell Sci., January 10, 2000; 113(19): 3365 - 3374. [Abstract] [PDF]
|
|
|
|
|
|
K. Toyo-oka, Y. Yashiro-Ohtani, C.-S. Park, X.-G. Tai, K. Miyake, T. Hamaoka, and H. Fujiwara Association of a tetraspanin CD9 with CD5 on the T cell surface: role of particular transmembrane domains in the association Int. Immunol., December 1, 1999; 11(12): 2043 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Berditchevski and E. Odintsova Characterization of Integrin–Tetraspanin Adhesion Complexes: Role of Tetraspanins in Integrin Signaling J. Cell Biol., July 26, 1999; 146(2): 477 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hammond, L. K. Denzin, M. Pan, J. M. Griffith, H. J. Geuze, and P. Cresswell The Tetraspan Protein CD82 Is a Resident of MHC Class II Compartments Where It Associates with HLA-DR, -DM, and -DO Molecules J. Immunol., October 1, 1998; 161(7): 3282 - 3291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hasegawa, T. Nomura, K. Kishimoto, K. Yanagisawa, and S. Fujita SFA-1/PETA-3 (CD151), a Member of the Transmembrane 4 Superfamily, Associates Preferentially with {alpha}5{beta}1 Integrin and Regulates Adhesion of Human T Cell Leukemia Virus Type 1-Infected T Cells to Fibronectin J. Immunol., September 15, 1998; 161(6): 3087 - 3095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Escola, M. J. Kleijmeer, W. Stoorvogel, J. M. Griffith, O. Yoshie, and H. J. Geuze Selective Enrichment of Tetraspan Proteins on the Internal Vesicles of Multivesicular Endosomes and on Exosomes Secreted by Human B-lymphocytes J. Biol. Chem., August 7, 1998; 273(32): 20121 - 20127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Fleming, E. Donnadieu, C. H. Song, F. V. Laethem, S. J. Galli, and J.-P. Kinet Negative Regulation of Fcepsilon RI-mediated Degranulation by CD81 J. Exp. Med., October 20, 1997; 186(8): 1307 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. N. Tsitsikov, J. C. Gutierrez-Ramos, and R. S. Geha Impaired CD19 expression and signaling, enhanced antibody response to type II T independent antigen and reduction of B-1 cells in CD81-deficient mice PNAS, September 30, 1997; 94(20): 10844 - 10849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Brown, N. H. Moskowitz, N. Killeen, and S. L. Reiner A Role for CD4 in Peripheral T Cell Differentiation J. Exp. Med., July 7, 1997; 186(1): 101 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Imai, T. Yoshida, M. Baba, M. Nishimura, M. Kakizaki, and O. Yoshie Molecular Cloning of a Novel T Cell-directed CC Chemokine Expressed in Thymus by Signal Sequence Trap Using Epstein-Barr Virus Vector J. Biol. Chem., August 30, 1996; 271(35): 21514 - 21521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Claas, C. S. Stipp, and M. E. Hemler Evaluation of Prototype Transmembrane 4 Superfamily Protein Complexes and Their Relation to Lipid Rafts J. Biol. Chem., March 9, 2001; 276(11): 7974 - 7984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Stipp, D. Orlicky, and M. E. Hemler FPRP, a Major, Highly Stoichiometric, Highly Specific CD81- and CD9-associated Protein J. Biol. Chem., February 9, 2001; 276(7): 4853 - 4862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Berditchevski, E. Gilbert, M. R. Griffiths, S. Fitter, L. Ashman, and S. J. Jenner Analysis of the CD151{middle dot}alpha 3beta 1 Integrin and CD151{middle dot}Tetraspanin Interactions by Mutagenesis J. Biol. Chem., October 26, 2001; 276(44): 41165 - 41174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Stipp, T. V. Kolesnikova, and M. E. Hemler EWI-2 Is a Major CD9 and CD81 Partner and Member of a Novel Ig Protein Subfamily J. Biol. Chem., October 26, 2001; 276(44): 40545 - 40554. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
