The Journal of Immunology, Vol 155, Issue 2 902-908, Copyright © 1995 by American Association of Immunologists

ARTICLES

Transcriptional activation of the human TNF-alpha promoter by superantigen in human monocytic cells: role of NF-kappa B

NS Trede, AV Tsytsykova, T Chatila, AE Goldfeld and RS Geha
Division of Immunology, Children's Hospital, Boston, MA 02115, USA.

We have studied the transcriptional activation of the human TNF-alpha gene by the superantigen staphylococcal enterotoxin A (SEA) in the human premonocytic cell line THP-1. Nuclear proteins from SEA- stimulated THP-1 cells bound strongly to kappa 3, the most proximal of three putative NF-kappa B binding sites (kappa 1-kappa 3) found in the 5' regulatory region of the TNF-alpha gene, but only weakly to kappa 1, the most distal of the NF-kappa B binding sites, and showed no binding to kappa 2. The mobility of the kappa 3-nucleoprotein complex was identical to that of complexes formed between nuclear proteins and the consensus NF-kappa B seuqence. Moreover, both 5' and 3' mutants of kappa 3 were unable to displace kappa 3 binding, suggesting that the kappa 3 binding complex induced by SEA has the characteristics of NF- kappa B. Studies using Abs directed against the NF-kappa B subunits p50 and p65 suggested that both p50 and p65 bind to the kappa 3 sequence. Reporter gene assays showed that deletion of kappa 3 (-99 to -89 bp) and point mutation of the three 5' guanine bases in the kappa 3 sequence reduced the inducibility of the TNF-alpha promoter by SEA and LPS. These results indicate that superantigen induces NF-kappa B in human monocytic cells and suggest that binding of NF-kappa B to the kappa 3 site of the TNF-alpha promoter plays an important role in the transcriptional activation of the TNF-alpha gene by superantigen.

This article has been cited by other articles:

				S. J. Skinner, K. M. Deleault, R. Fecteau, and S. A. Brooks Extracellular Signal-regulated Kinase Regulation of Tumor Necrosis Factor-{alpha} mRNA Nucleocytoplasmic Transport Requires TAP-NxT1 Binding and the AU-rich Element J. Biol. Chem., February 8, 2008; 283(6): 3191 - 3199. [Abstract] [Full Text] [PDF]

				M. E. Gazzar, B. K. Yoza, J. Y.-Q. Hu, S. L. Cousart, and C. E. McCall Epigenetic Silencing of Tumor Necrosis Factor {alpha} during Endotoxin Tolerance J. Biol. Chem., September 14, 2007; 282(37): 26857 - 26864. [Abstract] [Full Text] [PDF]

				K. E. Sullivan, A. B. M. Reddy, K. Dietzmann, A. R. Suriano, V. P. Kocieda, M. Stewart, and M. Bhatia Epigenetic Regulation of Tumor Necrosis Factor Alpha Mol. Cell. Biol., July 15, 2007; 27(14): 5147 - 5160. [Abstract] [Full Text] [PDF]

				A. V. Tsytsykova, J. V. Falvo, M. Schmidt-Supprian, G. Courtois, D. Thanos, and A. E. Goldfeld Post-induction, Stimulus-specific Regulation of Tumor Necrosis Factor mRNA Expression J. Biol. Chem., April 20, 2007; 282(16): 11629 - 11638. [Abstract] [Full Text] [PDF]

				F. O. Baxter, P. J. Came, K. Abell, B. Kedjouar, M. Huth, K. Rajewsky, M. Pasparakis, and C. J. Watson IKK{beta}/2 induces TWEAK and apoptosis in mammary epithelial cells Development, September 1, 2006; 133(17): 3485 - 3494. [Abstract] [Full Text] [PDF]

				A. R. Suriano, A. N. Sanford, N. Kim, M. Oh, S. Kennedy, M. J. Henderson, K. Dietzmann, and K. E. Sullivan GCF2/LRRFIP1 Represses Tumor Necrosis Factor Alpha Expression Mol. Cell. Biol., October 15, 2005; 25(20): 9073 - 9081. [Abstract] [Full Text] [PDF]

				P. A. Hopkins, J. D. Fraser, A. C. Pridmore, H. H. Russell, R. C. Read, and S. Sriskandan Superantigen recognition by HLA class II on monocytes up-regulates toll-like receptor 4 and enhances proinflammatory responses to endotoxin Blood, May 1, 2005; 105(9): 3655 - 3662. [Abstract] [Full Text] [PDF]

				Y. Zhao, S. Joshi-Barve, S. Barve, and L. H. Chen Eicosapentaenoic Acid Prevents LPS-Induced TNF-{alpha} Expression by Preventing NF-{kappa}B Activation J. Am. Coll. Nutr., February 1, 2004; 23(1): 71 - 78. [Abstract] [Full Text] [PDF]

				G. Lagoumintzis, M. Christofidou, G. Dimitracopoulos, and F. Paliogianni Pseudomonas aeruginosa Slime Glycolipoprotein Is a Potent Stimulant of Tumor Necrosis Factor Alpha Gene Expression and Activation of Transcription Activators Nuclear Factor {kappa}B and Activator Protein 1 in Human Monocytes Infect. Immun., August 1, 2003; 71(8): 4614 - 4622. [Abstract] [Full Text] [PDF]

				G. Stoecklin, M. Lu, B. Rattenbacher, and C. Moroni A Constitutive Decay Element Promotes Tumor Necrosis Factor Alpha mRNA Degradation via an AU-Rich Element-Independent Pathway Mol. Cell. Biol., May 15, 2003; 23(10): 3506 - 3515. [Abstract] [Full Text] [PDF]

				S. K. De, K. Devadas, and A. L. Notkins Elevated Levels of Tumor Necrosis Factor Alpha (TNF-{alpha}) in Human Immunodeficiency Virus Type 1-Transgenic Mice: Prevention of Death by Antibody to TNF-{alpha} J. Virol., October 11, 2002; 76(22): 11710 - 11714. [Abstract] [Full Text] [PDF]

				Y. Fu, K. K. Ishii, Y. Munakata, T. Saitoh, M. Kaku, and T. Sasaki Regulation of Tumor Necrosis Factor Alpha Promoter by Human Parvovirus B19 NS1 through Activation of AP-1 and AP-2 J. Virol., May 3, 2002; 76(11): 5395 - 5403. [Abstract] [Full Text] [PDF]

				P. W. Finn, J. R. Stone, M. R. Boothby, and D. L. Perkins Inhibition of NF-{kappa}B-Dependent T Cell Activation Abrogates Acute Allograft Rejection J. Immunol., November 15, 2001; 167(10): 5994 - 6001. [Abstract] [Full Text] [PDF]

				J. Warwick-Davies, A. J. Watson, G. E. Griffin, S. Krishna, and R. J. Shattock Enhancement of Mycobacterium tuberculosis-Induced Tumor Necrosis Factor Alpha Production from Primary Human Monocytes by an Activated T-Cell Membrane-Mediated Mechanism Infect. Immun., November 1, 2001; 69(11): 6580 - 6587. [Abstract] [Full Text] [PDF]

				A.-K. Yi, J.-G. Yoon, S.-C. Hong, T. W. Redford, and A. M. Krieg Lipopolysaccharide and CpG DNA synergize for tumor necrosis factor-{alpha} production through activation of NF-{kappa}B Int. Immunol., November 1, 2001; 13(11): 1391 - 1404. [Abstract] [Full Text] [PDF]

				M. Guha, M. A. O'Connell, R. Pawlinski, A. Hollis, P. McGovern, S.-F. Yan, D. Stern, and N. Mackman Lipopolysaccharide activation of the MEK-ERK1/2 pathway in human monocytic cells mediates tissue factor and tumor necrosis factor {alpha} expression by inducing Elk-1 phosphorylation and Egr-1 expression Blood, September 1, 2001; 98(5): 1429 - 1439. [Abstract] [Full Text] [PDF]

				S. Ellermann-Eriksen and V. Kruys Expression of TNF-{alpha} by Herpes Simplex Virus-Infected Macrophages Is Regulated by a Dual Mechanism: Transcriptional Regulation by NF-{kappa}B and Activating Transcription Factor 2/Jun and Translational Regulation Through the AU-Rich Region of the 3' Untranslated Region J. Immunol., August 15, 2001; 167(4): 2202 - 2208. [Abstract] [Full Text] [PDF]

				J. Y. Lee and K. E. Sullivan Gamma Interferon and Lipopolysaccharide Interact at the Level of Transcription To Induce Tumor Necrosis Factor Alpha Expression Infect. Immun., May 1, 2001; 69(5): 2847 - 2852. [Abstract] [Full Text] [PDF]

				E. Y. Tsai, J. V. Falvo, A. V. Tsytsykova, A. K. Barczak, A. M. Reimold, L. H. Glimcher, M. J. Fenton, D. C. Gordon, I. F. Dunn, and A. E. Goldfeld A Lipopolysaccharide-Specific Enhancer Complex Involving Ets, Elk-1, Sp1, and CREB Binding Protein and p300 Is Recruited to the Tumor Necrosis Factor Alpha Promoter In Vivo Mol. Cell. Biol., August 15, 2000; 20(16): 6084 - 6094. [Abstract] [Full Text]

				S. M. Weber and S. M. Levitz Chloroquine Interferes with Lipopolysaccharide-Induced TNF-{alpha} Gene Expression by a Nonlysosomotropic Mechanism J. Immunol., August 1, 2000; 165(3): 1534 - 1540. [Abstract] [Full Text] [PDF]

				J. Y. Leung, F. E. McKenzie, A. M. Uglialoro, P. O. Flores-Villanueva, B. C. Sorkin, E. J. Yunis, D. L. Hartl, and A. E. Goldfeld Identification of phylogenetic footprints in primate tumor necrosis factor-alpha promoters PNAS, June 6, 2000; 97(12): 6614 - 6618. [Abstract] [Full Text] [PDF]

				S. Takashiba, T. E. Van Dyke, S. Amar, Y. Murayama, A. W. Soskolne, and L. Shapira Differentiation of Monocytes to Macrophages Primes Cells for Lipopolysaccharide Stimulation via Accumulation of Cytoplasmic Nuclear Factor kappa B Infect. Immun., November 1, 1999; 67(11): 5573 - 5578. [Abstract] [Full Text] [PDF]

				S. Kastenbauer and H. W. L. Ziegler-Heitbrock NF-kappa B1 (p50) Is Upregulated in Lipopolysaccharide Tolerance and Can Block Tumor Necrosis Factor Gene Expression Infect. Immun., April 1, 1999; 67(4): 1553 - 1559. [Abstract] [Full Text] [PDF]

				D. V. Kuprash, I. A. Udalova, R. L. Turetskaya, D. Kwiatkowski, N. R. Rice, and S. A. Nedospasov Similarities and Differences Between Human and Murine TNF Promoters in Their Response to Lipopolysaccharide J. Immunol., April 1, 1999; 162(7): 4045 - 4052. [Abstract] [Full Text] [PDF]

				U. R. Pendurthi, J. T. Williams, and L. V. M. Rao Resveratrol, a Polyphenolic Compound Found in Wine, Inhibits Tissue Factor Expression in Vascular Cells : A Possible Mechanism for the Cardiovascular Benefits Associated With Moderate Consumption of Wine Arterioscler. Thromb. Vasc. Biol., February 1, 1999; 19(2): 419 - 426. [Abstract] [Full Text] [PDF]

				M. BAER, S. NEDOSPASOV, and P.F. JOHNSON Attenuation of Tumor Necrosis Factor {alpha} Gene Transcription in Macrophages by an Autocrine Factor Cold Spring Harb Symp Quant Biol, January 1, 1999; 64(0): 437 - 444. [Abstract] [PDF]

				F. Chen, V. Castranova, X. Shi, and L. M. Demers New Insights into the Role of Nuclear Factor-{kappa}B, a Ubiquitous Transcription Factor in the Initiation of Diseases Clin. Chem., January 1, 1999; 45(1): 7 - 17. [Abstract] [Full Text] [PDF]

				M. Delgado, E. J. Munoz-Elias, Y. Kan, I. Gozes, M. Fridkin, D. E. Brenneman, R. P. Gomariz, and D. Ganea Vasoactive Intestinal Peptide and Pituitary Adenylate Cyclase-activating Polypeptide Inhibit Tumor Necrosis Factor alpha  Transcriptional Activation by Regulating Nuclear Factor-kB and cAMP Response Element-binding Protein/c-Jun J. Biol. Chem., November 20, 1998; 273(47): 31427 - 31436. [Abstract] [Full Text] [PDF]

				N. Fujisawa, S. Hayashi, A. Kurdowska, J. M. Noble, K. Naitoh, and E. J. Miller Staphylococcal Enterotoxin A-Induced Injury of Human Lung Endothelial Cells and IL-8 Accumulation Are Mediated by TNF-{alpha} J. Immunol., November 15, 1998; 161(10): 5627 - 5632. [Abstract] [Full Text] [PDF]

				M. Baer, A. Dillner, R. C. Schwartz, C. Sedon, S. Nedospasov, and P. F. Johnson Tumor Necrosis Factor Alpha Transcription in Macrophages Is Attenuated by an Autocrine Factor That Preferentially Induces NF-kappa B p50 Mol. Cell. Biol., October 1, 1998; 18(10): 5678 - 5689. [Abstract] [Full Text]

				I. A. Udalova, J. C. Knight, V. Vidal, S. A. Nedospasov, and D. Kwiatkowski Complex NF-kappa B Interactions at the Distal Tumor Necrosis Factor Promoter Region in Human Monocytes J. Biol. Chem., August 14, 1998; 273(33): 21178 - 21186. [Abstract] [Full Text] [PDF]

				R. Sakiri, B. Ramegowda, and V. L. Tesh Shiga Toxin Type 1 Activates Tumor Necrosis Factor-alpha Gene Transcription and Nuclear Translocation of the Transcriptional Activators Nuclear Factor-kappa B and Activator Protein-1 Blood, July 15, 1998; 92(2): 558 - 566. [Abstract] [Full Text] [PDF]

				D. R. Meldrum Tumor necrosis factor in the heart Am J Physiol Regulatory Integrative Comp Physiol, March 1, 1998; 274(3): R577 - R595. [Abstract] [Full Text] [PDF]

				J. Yao, N. Mackman, T. S. Edgington, and S.-T. Fan Lipopolysaccharide Induction of the Tumor Necrosis Factor-alpha Promoter in Human Monocytic Cells. REGULATION BY Egr-1, c-Jun, AND NF-kappa B TRANSCRIPTION FACTORS J. Biol. Chem., July 11, 1997; 272(28): 17795 - 17801. [Abstract] [Full Text] [PDF]

				V. N. Ivanov and V. N. Ivanov Transcription Factor Activation during Signal-induced Apoptosis of Immature CD4+CD8+ Thymocytes. A PROTECTIVE ROLE OF c-Fos J. Biol. Chem., March 28, 1997; 272(13): 8558 - 8566. [Abstract] [Full Text] [PDF]

				M. Delgado and D. Ganea Vasoactive Intestinal Peptide and Pituitary Adenylate Cyclase-activating Polypeptide Inhibit Nuclear Factor-kappa B-dependent Gene Activation at Multiple Levels in the Human Monocytic Cell Line THP-1 J. Biol. Chem., January 5, 2001; 276(1): 369 - 380. [Abstract] [Full Text] [PDF]

				J. H. Steer, K. M. Kroeger, L. J. Abraham, and D. A. Joyce Glucocorticoids Suppress Tumor Necrosis Factor-alpha Expression by Human Monocytic THP-1 Cells by Suppressing Transactivation through Adjacent NF-kappa B and c-Jun-Activating Transcription Factor-2 Binding Sites in the Promoter J. Biol. Chem., June 9, 2000; 275(24): 18432 - 18440. [Abstract] [Full Text] [PDF]