| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 155, Issue 12 5637-5646, Copyright © 1995 by American Association of Immunologists
ARTICLES |
WD Warren and MT Berton
Department of Microbiology, University of Texas Health Science Center at San Antonio 78284, USA.
The interaction between B cell CD40 and its ligand (CD40L) on activated Th cells provides a critical signal necessary for T cell-dependent isotype switching. Previous studies suggest that this signal might be important in regulating isotype switching at the level of germ-line Ig transcription. To assess the effects of the CD40L-CD40 interaction on germ-line Ig transcript expression in murine B cells, a membrane-bound form of mouse CD40L was expressed in the baculovirus system. We show that stimulation of resting splenic B cells with CD40L-expressing Sf9 cells induces germ-line gamma 1 and epsilon transcripts independently of cytokines. The CD40-mediated induction cannot be blocked by anti-IL- 4 Ab and is not mediated by other cytokines secreted endogenously in response to CD40 stimulation. Importantly, stimulation with CD40L and IL-4 together has a significant synergistic effect on germ-line transcript expression. Stimulation of CD40 does not activate the NF-IL- 4-gamma 1 DNA binding factor believed to be required for IL-4-dependent germ-line gamma 1 transcription. Moreover, mutation of the NF-IL-4- gamma 1 DNA binding site in a germ-line gamma 1 promoter-luciferase reporter gene construct completely ablates IL-4 responsiveness but has no effect on responsiveness to CD40L in transient transfection assays. These results demonstrate that the CD40L-CD40 interaction and IL-4 activate germ-line Ig gene transcription by distinct but synergistic mechanisms and suggest that multiple signals may be required to induce sufficient germ-line transcription and/or germ-line transcript levels necessary to target switch recombination.
This article has been cited by other articles:
|
|
 |
|
  M. E. Munroe and G. A. Bishop A Costimulatory Function for T Cell CD40 J. Immunol., January 15, 2007; 178(2): 671 - 682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Collins, J. Shi, B. E. Burrell, D. K. Bishop, and W. A. Dunnick Induced Expression of Murine {gamma}2a by CD40 Ligation Independently of IFN-{gamma} J. Immunol., October 15, 2006; 177(8): 5414 - 5419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Valineva, J. Yang, and O. Silvennoinen Characterization of RNA helicase A as component of STAT6-dependent enhanceosome Nucleic Acids Res., September 1, 2006; 34(14): 3938 - 3946. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Valineva, J. Yang, R. Palovuori, and O. Silvennoinen The Transcriptional Co-activator Protein p100 Recruits Histone Acetyltransferase Activity to STAT6 and Mediates Interaction between the CREB-binding Protein and STAT6 J. Biol. Chem., April 15, 2005; 280(15): 14989 - 14996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Munroe and G. A. Bishop Role of Tumor Necrosis Factor (TNF) Receptor-associated Factor 2 (TRAF2) in Distinct and Overlapping CD40 and TNF Receptor 2/CD120b-mediated B Lymphocyte Activation J. Biol. Chem., December 17, 2004; 279(51): 53222 - 53231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Berton, L. A. Linehan, K. R. Wick, and W. A. Dunnick NF-{kappa}B elements associated with the Stat6 site in the germline {gamma}1 immunoglobulin promoter are not necessary for the transcriptional response to CD40 ligand Int. Immunol., December 1, 2004; 16(12): 1741 - 1749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. A. Dunnick, J. Shi, K. A. Graves, and J. T. Collins Germline Transcription and Switch Recombination of a Transgene Containing the Entire H Chain Constant Region Locus: Effect of a Mutation in a STAT6 Binding Site in the {gamma}1 Promoter J. Immunol., November 1, 2004; 173(9): 5531 - 5539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. O. Lee, J. Moyron-Quiroz, J. Rangel-Moreno, K. L. Kusser, L. Hartson, F. Sprague, F. E. Lund, and T. D. Randall CD40, but Not CD154, Expression on B Cells Is Necessary for Optimal Primary B Cell Responses J. Immunol., December 1, 2003; 171(11): 5707 - 5717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Kasprowicz, P. S. Smallwood, A. J. Tyznik, and S. F. Ziegler Scurfin (FoxP3) Controls T-Dependent Immune Responses In Vivo Through Regulation of CD4+ T Cell Effector Function J. Immunol., August 1, 2003; 171(3): 1216 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Podojil and V. M. Sanders Selective Regulation of Mature IgG1 Transcription by CD86 and {beta}2-Adrenergic Receptor Stimulation J. Immunol., May 15, 2003; 170(10): 5143 - 5151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pesu, S. Aittomaki, K. Takaluoma, A. Lagerstedt, and O. Silvennoinen p38 Mitogen-activated Protein Kinase Regulates Interleukin-4-induced Gene Expression by Stimulating STAT6-mediated Transcription J. Biol. Chem., October 4, 2002; 277(41): 38254 - 38261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. Hostager and G. A. Bishop Role of TNF Receptor-Associated Factor 2 in the Activation of IgM Secretion by CD40 and CD120b J. Immunol., April 1, 2002; 168(7): 3318 - 3322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Martinez-Barnetche, V. Madrid-Marina, R. A. Flavell, and J. Moreno Does CD40 Ligation Induce B Cell Negative Selection? J. Immunol., February 1, 2002; 168(3): 1042 - 1049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Litterst and E. Pfitzner Transcriptional Activation by STAT6 Requires the Direct Interaction with NCoA-1 J. Biol. Chem., November 30, 2001; 276(49): 45713 - 45721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. C. de Lafaille, S. Muriglan, M.-J. Sunshine, Y. Lei, N. Kutchukhidze, G. C. Furtado, A. K. Wensky, D. Olivares-Villagomez, and J. J. Lafaille Hyper Immunoglobulin E Response in Mice with Monoclonal Populations of B and T Lymphocytes"" J. Exp. Med., November 5, 2001; 194(9): 1349 - 1360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rabah, S. Grant, C. Ma, and D. H. Conrad Bryostatin-1 Specifically Inhibits In Vitro IgE Synthesis J. Immunol., November 1, 2001; 167(9): 4910 - 4918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Laurencikiene, V. Deveikaite, and E. Severinson HS1,2 Enhancer Regulation of Germline {epsilon} and {gamma}2b Promoters in Murine B Lymphocytes: Evidence for Specific Promoter-Enhancer Interactions J. Immunol., September 15, 2001; 167(6): 3257 - 3265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gao, T. Dang, and D. Yuan IFN-{gamma}-Dependent and -Independent Initiation of Switch Recombination by NK Cells J. Immunol., August 15, 2001; 167(4): 2011 - 2018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-s. Mao and J. Stavnezer Differential Regulation of Mouse Germline Ig {gamma}1 and {epsilon} Promoters by IL-4 and CD40 J. Immunol., August 1, 2001; 167(3): 1522 - 1534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. D. Erickson, T. M. Foy, and T. J. Waldschmidt Murine B1 B Cells Require IL-5 for Optimal T Cell-Dependent Activation J. Immunol., February 1, 2001; 166(3): 1531 - 1539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Shen and J. Stavnezer Activation of the Mouse Ig Germline {{epsilon}} Promoter by IL-4 Is Dependent on AP-1 Transcription Factors J. Immunol., January 1, 2001; 166(1): 411 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. F. Webb, Y. Yamashita, N. Ayers, S. Evetts, Y. Paulin, M. E. Conley, and E. A. Smith The Transcription Factor Bright Associates with Bruton's Tyrosine Kinase, the Defective Protein in Immunodeficiency Disease J. Immunol., December 15, 2000; 165(12): 6956 - 6965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Adams, H. Ackerly, K. Cunningham, and W. Dunnick A DNase I hypersensitive site near the murine {gamma}1 switch region contributes to insertion site independence of transgenes and modulates the amount of transcripts induced by CD40 ligation Int. Immunol., December 1, 2000; 12(12): 1705 - 1713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Kasprowicz, A. P. Kohm, M. T. Berton, A. J. Chruscinski, A. Sharpe, and V. M. Sanders Stimulation of the B Cell Receptor, CD86 (B7-2), and the {beta}2-Adrenergic Receptor Intrinsically Modulates the Level of IgG1 and IgE Produced per B Cell J. Immunol., July 15, 2000; 165(2): 680 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Davey, G. Greicius, J. Thyberg, and E. Severinson STAT6 is required for the regulation of IL-4-induced cytoskeletal events in B cells Int. Immunol., July 1, 2000; 12(7): 995 - 1003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Collins and W. A. Dunnick Cutting Edge: IFN-{gamma} Regulated Germline Transcripts Are Expressed from {gamma}2a Transgenes Independently of the Heavy Chain 3' Enhancers J. Immunol., December 1, 1999; 163(11): 5758 - 5762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Faquim-Mauro, R. L. Coffman, I. A. Abrahamsohn, and M. S. Macedo Cutting Edge: Mouse IgG1 Antibodies Comprise Two Functionally Distinct Types That Are Differentially Regulated by IL-4 and IL-12 J. Immunol., October 1, 1999; 163(7): 3572 - 3576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Soro, P. Morales-A., J. A. Martinez-M., S. Morales-A., S. G. Copin, M. A. R. Marcos, and M.-L. Gaspar Differential Involvement of the Transcription Factor Blimp-1 in T Cell-Independent and -Dependent B Cell Differentiation to Plasma Cells J. Immunol., July 15, 1999; 163(2): 611 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Spieker-Polet, P.-C. Yam, Z. Arbieva, S.-K. Zhai, and K. L. Knight In Vitro Induction of the Expression of Multiple IgA Isotype Genes in Rabbit B Cells by TGF-{beta} and IL-2 J. Immunol., May 1, 1999; 162(9): 5380 - 5388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hsing and G. A. Bishop Requirement for Nuclear Factor-{kappa}B Activation by a Distinct Subset of CD40-Mediated Effector Functions in B Lymphocytes J. Immunol., March 1, 1999; 162(5): 2804 - 2811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-C. Peakman and N. Maizels Localization of Splenic B Cells Activated for Switch Recombination by In Situ Hybridization with I{gamma}1 Switch Transcript and Rad51 Probes J. Immunol., October 15, 1998; 161(8): 4008 - 4015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-C. Lin, H. H. Wortis, and J. Stavnezer The Ability of CD40L, but Not Lipopolysaccharide, To Initiate Immunoglobulin Switching to Immunoglobulin G1 Is Explained by Differential Induction of NF-kappa B/Rel Proteins Mol. Cell. Biol., September 1, 1998; 18(9): 5523 - 5532. [Abstract] [Full Text]
|
|
|
|
|
|
J. D. Jeppson, H. R. Patel, N. Sakata, J. Domenico, N. Terada, and E. W. Gelfand Requirement for Dual Signals by Anti-CD40 and IL-4 for the Induction of Nuclear Factor-{kappa}B, IL-6, and IgE in Human B Lymphocytes J. Immunol., August 15, 1998; 161(4): 1738 - 1742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wykes, A. Pombo, C. Jenkins, and G. G. MacPherson Dendritic Cells Interact Directly with Naive B Lymphocytes to Transfer Antigen and Initiate Class Switching in a Primary T-Dependent Response J. Immunol., August 1, 1998; 161(3): 1313 - 1319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ballantyne, D. L. Henry, J. R. Muller, F. Briere, C. M. Snapper, M. Kehry, and K. B. Marcu Efficient Recombination of a Switch Substrate Retrovector in CD40-Activated B Lymphocytes: Implications for the Control of CH Gene Switch Recombination J. Immunol., August 1, 1998; 161(3): 1336 - 1347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Linehan, W. D. Warren, P. A. Thompson, M. J. Grusby, and M. T. Berton STAT6 Is Required for IL-4-Induced Germline Ig Gene Transcription and Switch Recombination J. Immunol., July 1, 1998; 161(1): 302 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Shen and J. Stavnezer Interaction of Stat6 and NF-kappa B: Direct Association and Synergistic Activation of Interleukin-4-Induced Transcription Mol. Cell. Biol., June 1, 1998; 18(6): 3395 - 3404. [Abstract] [Full Text]
|
|
|
|
|
|
K.-M. Toellner, S. A. Luther, D. M.-Y. Sze, R. K.-W. Choy, D. R. Taylor, I. C.M. MacLennan, and H. Acha-Orbea T Helper 1 (Th1) and Th2 Characteristics Start to Develop During T Cell Priming and Are Associated with an Immediate Ability to Induce Immunoglobulin Class Switching J. Exp. Med., April 20, 1998; 187(8): 1193 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Ford, C. H. Yin, B. Barnhart, K. Sztam, and L. R. Covey CD40 Ligand Exerts Differential Effects on the Expression of I{gamma} Transcripts in Subclones of an IgM+ Human B Cell Lymphoma Line J. Immunol., January 15, 1998; 160(2): 595 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ling and D. V. Goeddel MIP-T3, a Novel Protein Linking Tumor Necrosis Factor Receptor-associated Factor 3 to the Microtubule Network J. Biol. Chem., July 28, 2000; 275(31): 23852 - 23860. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
