The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Szente, B. E.
Right arrow Articles by Johnson, H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Szente, B. E.
Right arrow Articles by Johnson, H. M.

The Journal of Immunology, Vol 155, Issue 12 5617-5622, Copyright © 1995 by American Association of Immunologists

ARTICLES

Identification of IFN-gamma receptor binding sites for JAK2 and enhancement of binding by IFN-gamma and its C-terminal peptide IFN- gamma(95-133)

BE Szente, PS Subramaniam and HM Johnson
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611, USA.

The tyrosine kinase JAK2 is an integral part of the signal transduction pathways of a number of cytokines and growth factors, including IFN- gamma. Previously, we identified a species-nonspecific binding site for the C terminus of IFN-gamma, encompassed by IFN-gamma peptide IFN- gamma(95-133), on the membrane proximal region of the cytoplasmic domain of the IFN-gamma R alpha-chain. Using both a radioligand binding assay and coimmunoprecipitation with antireceptor antiserum, we were able to demonstrate specific interaction of JAK2 with the murine IFN- gamma R(MIR) alpha-chain. Furthermore, this interaction is increased by the addition of murine IFN-gamma or its C-terminal peptide, muIFN- gamma(95-133). We also identified two regions of the cytoplasmic domain of the receptor that interact with JAK2 using synthetic peptides of the MIR alpha-chain in receptor competition studies. These regions are encompassed by receptor peptide MIR(283-309), which is adjacent to the membrane proximal region at which the C terminus of IFN-gamma binds, and receptor peptide MIR(404-432), which lies near the C terminus of the receptor, encompassing a potentially important phosphorylation site. These data show site-specific interaction between JAK2 and IFN- gamma with the IFN-gamma R and have broader implications for the role of the IFN-gamma ligand in the IFN-gamma signal transduction pathway. Furthermore, the data support previous studies that demonstrated that intracellular IFN-gamma plays a role in cell activation.


This article has been cited by other articles:


Home page
 CVIHome page
M. G. Mujtaba, C. B. Patel, R. A. Patel, L. O. Flowers, M. A. Burkhart, L. W. Waiboci, J. Martin, M. I. Haider, C. M. Ahmed, and H. M. Johnson
The Gamma Interferon (IFN-{gamma}) Mimetic Peptide IFN-{gamma}(95-133) Prevents Encephalomyocarditis Virus Infection both in Tissue Culture and in Mice.
Clin. Vaccine Immunol., August 1, 2006; 13(8): 944 - 952.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. M. I. Ahmed and H. M. Johnson
IFN-{gamma} and Its Receptor Subunit IFNGR1 Are Recruited to the IFN-{gamma}-Activated Sequence Element at the Promoter Site of IFN-{gamma}-Activated Genes: Evidence of Transactivational Activity in IFNGR1
J. Immunol., July 1, 2006; 177(1): 315 - 321.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. M. I. Ahmed, M. A. Burkhart, P. S. Subramaniam, M. G. Mujtaba, and H. M. Johnson
Peptide Mimetics of Gamma Interferon Possess Antiviral Properties against Vaccinia Virus and Other Viruses in the Presence of Poxvirus B8R Protein
J. Virol., May 1, 2005; 79(9): 5632 - 5639.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. M. I. Ahmed, M. A. Burkhart, M. G. Mujtaba, P. S. Subramaniam, and H. M. Johnson
The role of IFN{gamma} nuclear localization sequence in intracellular function
J. Cell Sci., August 1, 2003; 116(15): 3089 - 3098.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. S. Subramaniam and H. M. Johnson
Lipid Microdomains Are Required Sites for the Selective Endocytosis and Nuclear Translocation of IFN-{gamma}, Its Receptor Chain IFN-{gamma} Receptor-1, and the Phosphorylation and Nuclear Translocation of STAT1{alpha}
J. Immunol., August 15, 2002; 169(4): 1959 - 1969.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Subramaniam, J Larkin, M. Mujtaba, M. Walter, and H. Johnson
The COOH-terminal nuclear localization sequence of interferon gamma regulates STAT1 alpha nuclear translocation at an intracellular site
J. Cell Sci., January 8, 2000; 113(15): 2771 - 2781.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
P. S. Subramaniam, M. G. Mujtaba, M. R. Paddy, and H. M. Johnson
The Carboxyl Terminus of Interferon-gamma Contains a Functional Polybasic Nuclear Localization Sequence
J. Biol. Chem., January 1, 1999; 274(1): 403 - 407.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.