The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fernandez, A.
Right arrow Articles by McConkey, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fernandez, A.
Right arrow Articles by McConkey, D. J.

The Journal of Immunology, Vol 155, Issue 11 5133-5139, Copyright © 1995 by American Association of Immunologists

ARTICLES

Oxygen radical production and thiol depletion are required for Ca(2+)- mediated endogenous endonuclease activation in apoptotic thymocytes

A Fernandez, J Kiefer, L Fosdick and DJ McConkey
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.

Glucocorticoid hormones stimulate apoptosis in thymocytes via a mechanism that involves changes in intracellular Ca2+, and exogenous Ca2+ can also directly promote the nuclear alterations of apoptosis (lamin degradation and chromatin cleavage) in isolated nuclei. Here we report that glucocorticoid treatment resulted in the production of reactive oxygen species and the depletion of reduced glutathione. Separation of apoptotic cells on Percoll gradients demonstrated that both effects selectively occurred in thymocytes undergoing apoptosis. Moreover, glucocorticoid-induced endonuclease activation was partially blocked by the antioxidant N-acetyl-L-cysteine. Although abrogation of methylprednisolone-induced Ca2+ increases using the intracellular Ca2+ buffer 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid resulted in inhibition of endonuclease activation, it failed to prevent GSH depletion. However, N-acetyl-L-cysteine almost completely blocked methylprednisolone-induced elevations in cytosolic calcium levels, indicating that oxidative stress was playing a role in the Ca2+ response. Our results support the idea that oxidative stress is a key component of the apoptotic effector pathway in thymocytes, and that it interacts, at least in part, with the Ca2+ response.


This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
D. R. Dobis, R. T. Sawyer, M. M. Gillespie, J. Huang, L. S. Newman, L. A. Maier, and B. J. Day
Modulation of Lymphocyte Proliferation by Antioxidants in Chronic Beryllium Disease
Am. J. Respir. Crit. Care Med., May 1, 2008; 177(9): 1002 - 1011.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. C. Davis, K. S. McColl, F. Zhong, Z. Wang, M. H. Malone, and C. W. Distelhorst
Dexamethasone-induced Inositol 1,4,5-Trisphosphate Receptor Elevation in Murine Lymphoma Cells Is Not Required for Dexamethasone-mediated Calcium Elevation and Apoptosis
J. Biol. Chem., April 18, 2008; 283(16): 10357 - 10365.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Franco and J. A. Cidlowski
SLCO/OATP-like Transport of Glutathione in FasL-induced Apoptosis: GLUTATHIONE EFFLUX IS COUPLED TO AN ORGANIC ANION EXCHANGE AND IS NECESSARY FOR THE PROGRESSION OF THE EXECUTION PHASE OF APOPTOSIS
J. Biol. Chem., October 6, 2006; 281(40): 29542 - 29557.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. Quadrilatero and L. Hoffman-Goetz
N-acetyl-l-cysteine protects intestinal lymphocytes from apoptotic death after acute exercise in adrenalectomized mice
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1664 - R1672.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Pullar and M. B. Hampton
Diphenyleneiodonium Triggers the Efflux of Glutathione from Cultured Cells
J. Biol. Chem., May 24, 2002; 277(22): 19402 - 19407.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
C. C. Franklin, C. M. Krejsa, R. H. Pierce, C. C. White, N. Fausto, and T. J. Kavanagh
Caspase-3-Dependent Cleavage of the Glutamate-L-Cysteine Ligase Catalytic Subunit during Apoptotic Cell Death
Am. J. Pathol., May 1, 2002; 160(5): 1887 - 1894.
[Abstract] [Full Text] [PDF]

Home page
 Cell Growth Differ.Home page
M. Zhang, M. J. Blake, P. W. Gout, D. J. Buckley, and A. R. Buckley
Proteolysis of Heat Shock Transcription Factor Is Associated with Apoptosis in Rat Nb2 Lymphoma Cells
Cell Growth Differ., November 1, 1999; 10(11): 759 - 767.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
J. R. Lee and G. A. Koretzky
Extracellular Signal-Regulated Kinase-2, But Not c-Jun NH2-Terminal Kinase, Activation Correlates with Surface IgM-Mediated Apoptosis in the WEHI 231 B Cell Line
J. Immunol., August 15, 1998; 161(4): 1637 - 1644.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. A. Herzenberg, S. C. De Rosa, J. G. Dubs, M. Roederer, M. T. Anderson, S. W. Ela, S. C. Deresinski, and L. A. Herzenberg
Glutathione deficiency is associated with impaired survival in HIV disease
PNAS, March 4, 1997; 94(5): 1967 - 1972.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-M. Kim, M. E. de Vera, S. C. Watkins, and T. R. Billiar
Nitric Oxide Protects Cultured Rat Hepatocytes from Tumor Necrosis Factor-alpha -induced Apoptosis by Inducing Heat Shock Protein 70Expression
J. Biol. Chem., January 10, 1997; 272(2): 1402 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
D. McConkey and S Orrenius
Signal transduction pathways in apoptosis
Stem Cells, November 1, 1996; 14(6): 619 - 631.
[Abstract]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.