The Journal of Immunology, Vol 154, Issue 6 2655-2667, Copyright © 1995 by American Association of Immunologists

ARTICLES

Immobilized anti-CD3 antibody activates T cell clones to induce the production of interstitial collagenase, but not tissue inhibitor of metalloproteinases, in monocytic THP-1 cells and dermal fibroblasts

AM Miltenburg, S Lacraz, HG Welgus and JM Dayer
Department of Medicine, University Hospital, Geneva, Switzerland.

In this study we have investigated whether direct cell to cell contact between activated paraformaldehyde-fixed T cell clones obtained from synovial tissue of patients with osteoarthritis (OA) or rheumatoid arthritis and target monocytic cells or dermal fibroblasts influenced the balance between interstitial collagenase and its specific inhibitor tissue inhibitor of metalloproteinases (TIMP) produced by the latter cell types. PHA/PMA-activated fixed T cell clones or their membranes strongly induced the production of collagenase both in monocytic THP-1 cells and in dermal fibroblasts. In contrast, only low levels of TIMP were induced in THP-1 cells and no change of TIMP expression was observed in fibroblasts as a result of stimulation with PHA/PMA- activated T cells or T cell membranes. Anti-CD3-activated T cell clones stimulated the production of collagenase both in THP-1 cells and fibroblasts, whereas TIMP levels were not influenced. Collagenase production in THP-1 cells induced by anti-CD3-activated T cell clones was 1) dependent on the dose of anti-CD3 used to stimulate the T cells, 2) initiated only when CD3 was cross-linked, and 3) inhibited when cyclosporin A was included during T cell activation. Our data collectively indicate that activated T cells in contact with monocytic cells or fibroblasts may alter the balance between interstitial collagenase and its specific inhibitor TIMP. This selective induction of a mediator profile representative of matrix breakdown as a result of target cell interaction with activated T cells may be an important factor in the local process of tissue destruction that characterizes osteoarthritis and rheumatoid arthritis.

This article has been cited by other articles:

				A. Scanu, N. Molnarfi, K. J. Brandt, L. Gruaz, J.-M. Dayer, and D. Burger Stimulated T cells generate microparticles, which mimic cellular contact activation of human monocytes: differential regulation of pro- and anti-inflammatory cytokine production by high-density lipoproteins J. Leukoc. Biol., April 1, 2008; 83(4): 921 - 927. [Abstract] [Full Text] [PDF]

				N. L. Webster and S. M. Crowe Matrix metalloproteinases, their production by monocytes and macrophages and their potential role in HIV-related diseases J. Leukoc. Biol., November 1, 2006; 80(5): 1052 - 1066. [Abstract] [Full Text] [PDF]

				J. L. Owen, V. Iragavarapu-Charyulu, Z. Gunja-Smith, L. M. Herbert, J. F. Grosso, and D. M. Lopez Up-Regulation of Matrix Metalloproteinase-9 in T Lymphocytes of Mammary Tumor Bearers: Role of Vascular Endothelial Growth Factor J. Immunol., October 15, 2003; 171(8): 4340 - 4351. [Abstract] [Full Text] [PDF]

				J-M. Dayer How T-lymphocytes are activated and become activators by cell{-}cell interaction Eur. Respir. J., September 20, 2003; 22(44_suppl): 10s - 15s. [Full Text] [PDF]

				K.-i. Shimokawa, M. Katayama, Y. Matsuda, H. Takahashi, I. Hara, H. Sato, and S. Kaneko Matrix metalloproteinase (MMP)-2 and MMP-9 activities in human seminal plasma Mol. Hum. Reprod., January 1, 2002; 8(1): 32 - 36. [Abstract] [Full Text] [PDF]

				N. Hyka, J.-M. Dayer, C. Modoux, T. Kohno, C. K. Edwards III, P. Roux-Lombard, and D. Burger Apolipoprotein A-I inhibits the production of interleukin-1{beta} and tumor necrosis factor-{alpha} by blocking contact-mediated activation of monocytes by T lymphocytes Blood, April 15, 2001; 97(8): 2381 - 2389. [Abstract] [Full Text] [PDF]

				N. M. Price, J. Farrar, T. T. H. Chau, N. T. H. Mai, T. T. Hien, and J. S. Friedland Identification of a Matrix-Degrading Phenotype in Human Tuberculosis In Vitro and In Vivo J. Immunol., March 15, 2001; 166(6): 4223 - 4230. [Abstract] [Full Text] [PDF]

				C. Chizzolini, R. Rezzonico, C. De Luca, D. Burger, and J.-M. Dayer Th2 Cell Membrane Factors in Association with IL-4 Enhance Matrix Metalloproteinase-1 (MMP-1) While Decreasing MMP-9 Production by Granulocyte-Macrophage Colony-Stimulating Factor-Differentiated Human Monocytes J. Immunol., June 1, 2000; 164(11): 5952 - 5960. [Abstract] [Full Text] [PDF]

				P. Giraudon, R. Szymocha, S. Buart, A. Bernard, L. Cartier, M.-F. Belin, and H. Akaoka T Lymphocytes Activated by Persistent Viral Infection Differentially Modify the Expression of Metalloproteinases and Their Endogenous Inhibitors, TIMPs, in Human Astrocytes: Relevance to HTLV-I-Induced Neurological Disease J. Immunol., March 1, 2000; 164(5): 2718 - 2727. [Abstract] [Full Text] [PDF]

				C. Phan, A. W. McMahon, R. C. Nelson, J. F. Elliott, and A. G. Murray Activated Lymphocytes Promote Endothelial Cell Detachment from Matrix: A Role for Modulation of Endothelial Cell {beta}1 Integrin Affinity J. Immunol., October 15, 1999; 163(8): 4557 - 4563. [Abstract] [Full Text] [PDF]

				R. Rezzonico, D. Burger, and J.-M. Dayer Direct Contact between T Lymphocytes and Human Dermal Fibroblasts or Synoviocytes Down-regulates Types I and III Collagen Production via Cell-associated Cytokines J. Biol. Chem., July 24, 1998; 273(30): 18720 - 18728. [Abstract] [Full Text] [PDF]

				L. I. Sakkas, C. Scanzello, N. Johanson, J. Burkholder, A. Mitra, P. Salgame, C. D. Katsetos, and C. D. Platsoucas T Cells and T-Cell Cytokine Transcripts in the Synovial Membrane in Patients with Osteoarthritis Clin. Vaccine Immunol., July 1, 1998; 5(4): 430 - 437. [Abstract] [Full Text]

				F. Aoudjit, E. F. Potworowski, and Y. St-Pierre Bi-Directional Induction of Matrix Metalloproteinase-9 and Tissue Inhibitor of Matrix Metalloproteinase-1 During T Lymphoma/Endothelial Cell Contact: Implication of ICAM-1 J. Immunol., March 15, 1998; 160(6): 2967 - 2973. [Abstract] [Full Text] [PDF]