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The Journal of Immunology, Vol 154, Issue 5 2075-2081, Copyright © 1995 by American Association of Immunologists

ARTICLES

T cell receptor alpha-chain defines the antigen specificity of antigen- specific suppressor factor but does not impart genetic restriction

RM O'Hara Jr, MC Byrne, VK Kuchroo, A Nagelin, MJ Whitters, S Jayaraman, SL Henderson, ME Dorf and M Collins
Genetics Institute, Cambridge, MA 02140.

Previous studies utilizing NP (4-hydroxy, 3-nitrophenyl acetyl hapten)- specific, T suppressor hybridomas have indicated that expression of TCR- alpha, but not TCR-beta, mRNA is required for expression of Ag-specific suppressor factor bioactivity. Suppressor-effector factor has been shown to be Ag specific and I-J restricted. Although the expression of TCR-alpha mRNA was necessary for suppressor activity, the role of TCR- alpha, as it pertained to the functional properties of T cell suppressor factor (TsF), was not established. To determine which properties of TsF could be accounted for by TCR-alpha expression, TCR- alpha cDNA, derived from NP-specific, suppressor T cell (Ts) hybridomas, was transfected into recipient Ts hybridomas of a second Ag specificity. The resulting heterologous transfectants displayed NP- specific, genetically restricted TsF activity. The Ag specificity corresponded to that of the TCR-alpha donor; however, the genetic restriction was influenced by the recipient cell, implying that TCR- alpha did not control genetic restriction of the TsF. Examination of TCR-beta expression in one of the MHC-restricted transfectants indicated that the genetic restriction of TsF could not be accounted for by TCR-beta gene products. The data support the conclusion that TCR- alpha expression is not only obligate for TsF bioactivity, but that the Ag specificity of the TCR-alpha dictates the Ag specificity of the resulting suppressor factor.


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