The Journal of Immunology, Vol 154, Issue 4 1804-1809, Copyright © 1995 by American Association of Immunologists

ARTICLES

Accumulation of distinct T cell clonotypes in human solid tumors

K Yamamoto, K Masuko, S Takahashi, Y Ikeda, T Kato, Y Mizushima, K Hayashi and K Nishioka
Division of Rheumatology and Molecular Immunology, St. Marianna University, School of Medicine, Kawasaki, Japan.

Tumor-infiltrating lymphocytes (TIL) have been described in a variety of human solid tumors. It is unknown whether such T cells are nonspecific inflammatory cells or a subset of specific host immune responses. To examine this question, we have analyzed the clonotypes of TCR beta-chain messages expressed in TIL, draining lymph nodes, and PBL of 10 patients with uterine or ovarian tumors. We report here that TIL bears distinct T cell clonotype accumulations only in patients without obvious metastasis. In contrast, accumulations of clonally expanded T cells were also found in lymph nodes and PBL of patients with metastatic cancer. The numbers and locations of the accumulated T cell clonotypes seemed to correlate with the stage of tumor invasion and the degree of metastasis. These data support the existence of Ag-driven immune responses to solid tumors in vivo.

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