The Journal of Immunology, Vol 154, Issue 3 1097-1104, Copyright © 1995 by American Association of Immunologists

ARTICLES

CD43 diminishes susceptibility to T lymphocyte-mediated cytolysis

TA McFarland, B Ardman, N Manjunath, JA Fabry and J Lieberman
Department of Medicine, New England Medical Center Hospitals, Boston, MA 02111.

CD43 is a major membrane sialoglycoprotein expressed by cells of hematopoietic origin. One property of CD43 is its ability to interfere with heterotypic and homotypic cellular adhesion. To determine whether CD43 expression can affect cell functions requiring intercellular adhesion, we compared a CD43-positive human T cell line (CEM) and its CD43-negative counterpart derived by gene targeting for susceptibility to cell-mediated lysis. CD43-negative CEM cells were more susceptible than CD43-positive cells to lysis by allospecific T cell lines derived from several donors. Induction of CD43 expression on transfected HeLa cells also imparted resistance to lectin-mediated lysis by a CD8+ T cell clone. The effect of CD43 expression on reducing susceptibility to lysis was more pronounced in short-term cytotoxicity assays and tended to disappear as the time of contact between the effector cell and its target increased. The enhanced susceptibility of CD43-negative cells to lysis was not associated with increased expression of adhesion molecules known to mediate antigen-independent cellular adhesion. Sialic acid residues on CD43 contributed to the CD43 protective effect. These results suggest that either diminished CD43 expression or incomplete sialylation may render hematopoietic cells more susceptible to T lymphocyte-mediated cytolysis.

This article has been cited by other articles:

				E. Gubina, T. Chen, L. Zhang, E. F. Lizzio, and S. Kozlowski CD43 polarization in unprimed T cells can be dissociated from raft coalescence by inhibition of HMG CoA reductase Blood, April 1, 2002; 99(7): 2518 - 2525. [Abstract] [Full Text] [PDF]

				F. Ulloa and F. X. Real Differential Distribution of Sialic Acid in {{alpha}}2,3 and {{alpha}}2,6 Linkages in the Apical Membrane of Cultured Epithelial Cells and Tissues J. Histochem. Cytochem., April 1, 2001; 49(4): 501 - 510. [Abstract] [Full Text]

				Y.-W. He and M. J. Bevan High Level Expression of CD43 Inhibits T Cell Receptor/CD3-mediated Apoptosis J. Exp. Med., December 20, 1999; 190(12): 1903 - 1908. [Abstract] [Full Text] [PDF]

				M. Nieto, J. L. Rodriguez-Fernandez, F. Navarro, D. Sancho, J. M.R. Frade, M. Mellado, C. Martinez-A, C. Cabanas, and F. Sanchez-Madrid Signaling Through CD43 Induces Natural Killer Cell Activation, Chemokine Release, and PYK-2 Activation Blood, October 15, 1999; 94(8): 2767 - 2777. [Abstract] [Full Text] [PDF]

				J. M. Serrador, M. Nieto, J. L. Alonso-Lebrero, M. A. del Pozo, J. Calvo, H. Furthmayr, R. Schwartz-Albiez, F. Lozano, R. Gonzalez-Amaro, P. Sanchez-Mateos, et al. CD43 Interacts With Moesin and Ezrin and Regulates Its Redistribution to the Uropods of T Lymphocytes at the Cell-Cell Contacts Blood, June 15, 1998; 91(12): 4632 - 4644. [Abstract] [Full Text] [PDF]