The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Elson, C. O.
Right arrow Articles by Anderson, A. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Elson, C. O.
Right arrow Articles by Anderson, A. O.

The Journal of Immunology, Vol 154, Issue 3 1032-1040, Copyright © 1995 by American Association of Immunologists

ARTICLES

Morphologic and functional alterations of mucosal T cells by cholera toxin and its B subunit

CO Elson, SP Holland, MT Dertzbaugh, CF Cuff and AO Anderson
Department of Medicine, University of Alabama at Birmingham 35294.

Despite the mucosal immunogenicity and adjuvanticity in vivo of cholera toxin (CT), both CT and CT B subunit are strong inhibitors of T cell activation in vitro. This study asked whether such T cell inhibition is relevant to the mucosal effects of CT in vivo. The activation of T cells pulsed in vitro for only 15 to 120 min with CT or CT B subunit, respectively, was inhibited, consistent with the expected short exposure times in vivo. Although both CD8+ and CD4+ T cells were inhibited in vitro, CD8+ T cells bound more toxin and were inhibited to a greater degree than were CD4+ T cells. Intestinal gavage of mice with 10 micrograms CT did not alter the overall composition of Peyer's Patch, mesenteric lymph node, or spleen but did cause a marked depletion of intraepithelial lymphocytes, mainly CD8+ T cells, and of lymphocytes in the dome epithelium over Peyer's Patch. To determine whether such inhibition of T cells was functionally relevant in vivo, T cells from mice fed keyhole limpet hemocyanin (KLH) were adoptively transferred into naive recipients, who were then parenterally immunized. T cells from mice fed KLH alone inhibited both the systemic IgG and secretory IgA anti-KLH response, but T cells from mice fed KLH plus CT did not, indicating that mucosally applied CT was able to abrogate the induction of this suppressor T cell. We conclude that one of the mechanisms of CT's mucosal effects in vivo is the inhibition of certain mucosal T cell functions and alteration of the regulatory T cell environment in gut-associated lymphoid tissue.


This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
G. Dixit, C. Mikoryak, T. Hayslett, A. Bhat, and R. K. Draper
Cholera Toxin Up-Regulates Endoplasmic Reticulum Proteins That Correlate with Sensitivity to the Toxin
Experimental Biology and Medicine, February 1, 2008; 233(2): 163 - 175.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
G. Hajishengallis, S. Arce, C.M. Gockel, T.D. Connell, and M.W. Russell
Immunomodulation with Enterotoxins for the Generation of Secretory Immunity or Tolerance: Applications for Oral Infections
J. Dent. Res., December 1, 2005; 84(12): 1104 - 1116.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. K. Rouquette-Jazdanian, A. Foussat, L. Lamy, C. Pelassy, P. Lagadec, J.-P. Breittmayer, and C. Aussel
Cholera Toxin B-Subunit Prevents Activation and Proliferation of Human CD4+ T Cells by Activation of a Neutral Sphingomyelinase in Lipid Rafts
J. Immunol., November 1, 2005; 175(9): 5637 - 5648.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C.-F. Flach, S. Lange, E. Jennische, I. Lonnroth, and J. Holmgren
Cholera Toxin Induces a Transient Depletion of CD8+ Intraepithelial Lymphocytes in the Rat Small Intestine as Detected by Microarray and Immunohistochemistry
Infect. Immun., September 1, 2005; 73(9): 5595 - 5602.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
A M Westendorf, M Templin, R Geffers, S Deppenmeier, A D Gruber, M Probst-Kepper, W Hansen, R S Liblau, F Gunzer, D Bruder, et al.
CD4+ T cell mediated intestinal immunity: chronic inflammation versus immune regulation
Gut, January 1, 2005; 54(1): 60 - 69.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. J. Salmond, R. Williams, T. R. Hirst, and N. A. Williams
The B Subunit of Escherichia coli Heat-Labile Enterotoxin Induces Both Caspase-Dependent and -Independent Cell Death Pathways in CD8+ T Cells
Infect. Immun., October 1, 2004; 72(10): 5850 - 5857.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
I. Castagliuolo, M. Sardina, P. Brun, C. DeRos, C. Mastrotto, L. Lovato, and G. Palu
Clostridium difficile Toxin A Carboxyl-Terminus Peptide Lacking ADP-Ribosyltransferase Activity Acts as a Mucosal Adjuvant
Infect. Immun., May 1, 2004; 72(5): 2827 - 2836.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. E. Laliberte, D. G. Perregaux, L. R. Hoth, P. J. Rosner, C. K. Jordan, K. M. Peese, James. F. Eggler, M. A. Dombroski, K. F. Geoghegan, and C. A. Gabel
Glutathione S-Transferase Omega 1-1 Is a Target of Cytokine Release Inhibitory Drugs and May Be Responsible for Their Effect on Interleukin-1beta Posttranslational Processing
J. Biol. Chem., May 2, 2003; 278(19): 16567 - 16578.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
H. Bone, S. Eckholdt, and N. A. Williams
Modulation of B lymphocyte signalling by the B subunit of Escherichia coli heat-labile enterotoxin
Int. Immunol., June 1, 2002; 14(6): 647 - 658.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Soriani, N. A. Williams, and T. R. Hirst
Escherichia coli Enterotoxin B Subunit Triggers Apoptosis of CD8+ T Cells by Activating Transcription Factor c-Myc
Infect. Immun., August 1, 2001; 69(8): 4923 - 4930.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. M. Lopes, A. Maroof, G. Dougan, and B. M. Chain
Inhibition of T-cell Response by Escherichia coli Heat-Labile Enterotoxin-Treated Epithelial Cells
Infect. Immun., December 1, 2000; 68(12): 6891 - 6895.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
M. C. Braun, J. He, C.-Y. Wu, and B. L. Kelsall
Cholera Toxin Suppresses Interleukin (IL)-12 Production and IL-12 Receptor beta 1 and beta 2 Chain Expression
J. Exp. Med., February 1, 1999; 189(3): 541 - 552.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. L. Truitt, C. Hanke, J. Radke, R. Mueller, and J. T. Barbieri
Glycosphingolipids as Novel Targets for T-Cell Suppression by the B Subunit of Recombinant Heat-Labile Enterotoxin
Infect. Immun., April 1, 1998; 66(4): 1299 - 1308.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Grdic, E. Hornquist, M. Kjerrulf, and N. Y. Lycke
Lack of Local Suppression in Orally Tolerant CD8-Deficient Mice Reveals a Critical Regulatory Role of CD8+ T Cells in the Normal Gut Mucosa
J. Immunol., January 15, 1998; 160(2): 754 - 762.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. A. Williams, L. M. Stasiuk, T. O. Nashar, C. M. Richards, A. K. Lang, M. J. Day, and T. R. Hirst
Prevention of autoimmune disease due to lymphocyte modulation by the B-subunit of Escherichia coli heat-labile enterotoxin
PNAS, May 13, 1997; 94(10): 5290 - 5295.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1995 by The American Association of Immunologists, Inc. All rights reserved.