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The Journal of Immunology, Vol 154, Issue 2 676-684, Copyright © 1995 by American Association of Immunologists

ARTICLES

Diversity of V gamma gene segments rearranged to the J gamma 4 gene in mice

N Atsuta, H Nishimura, N Nakamura, M Emoto, T Iwatsuki and Y Yoshikai
Laboratory of Germfree Life, Nagoya University School of Medicine, Japan.

Because there are limited numbers of V gamma gene segments and most V gamma rearrangements occur within clusters of the J gamma-C gamma genes in mice, gamma-chains display limited diversity compared with other TCR chains. In this study, we examined the nucleotide sequences of the V gamma-J gamma genes expressed in the gamma delta T cells appearing at the inflamed sites after Salmonella infection in DBA/2 mice. Most of the productive gamma gene rearrangements were V gamma 1-J gamma 4, whereas V gamma 2 and a unique V gamma, the 5' region of which was identical with sequences of the V gamma 2 gene, and the 3' region of which was identical with that of the V gamma 1 gene, were found to be rearranged to J gamma 4 gene, albeit at low frequency. Analysis of the ontogenic appearance of the rearrangements in the J gamma 4-C gamma 4 locus revealed that V gamma 2-J gamma 4 gene rearrangement was frequent in fetal thymocytes at the early stage of gestation. Most of the early fetal V gamma 2-J gamma 4 rearrangements exhibited the identical junction, a nonfunctional canonical sequence. The sequence analysis of the coding joint and the reciprocal recombination signal joint suggests that short homology-mediated direct recombination and chromosomal inversion mechanism are involved in fetal V gamma 2-J gamma 4 gene rearrangement. Taken together, our data suggest that the recombination of multiple V gamma segments with J gamma 4 can diversify the V gamma repertoire.


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Y. Naiki, H. Nishimura, T. Kawano, Y. Tanaka, S. Itohara, M. Taniguchi, and Y. Yoshikai
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J. Immunol., August 15, 1999; 163(4): 2057 - 2063.
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