| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 154, Issue 11 5927-5933, Copyright © 1995 by American Association of Immunologists
ARTICLES |
KW Marshall, KJ Wilson, J Liang, A Woods, D Zaller and JB Rothbard
ImmuLogic Pharmaceutical Corp., Palo Alto, CA 94305, USA.
A method to predict quantitatively peptide binding to HLA DRB1*0401 has been developed using a data set of the relative contributions of each of the naturally occurring amino acids in the context of a simplified peptide back-bone. The prediction assumed that the relative role of each of the peptide side chains could be treated independently and could be measured by assaying each of the 20 naturally occurring amino acids at the central 11 positions of a 13-residue peptide previously shown to contain the minimal requirements for high-affinity binding to HLA-DR proteins. The resultant database was shown to have predictive value when tested on a set of 13 unrelated peptides known to bind DRB1*0401 with a wide range of apparent affinity. The database was tested further by analyzing myelin basic protein. All 13 amino acid peptides containing a hydrophobic amino acid at the third position were synthesized and assayed for binding purified DRB1*0401. In every case, the measured affinity correlated with the predictive values within the experimental error of the assays. Finally, the ability to predict peptide binding to MHC class II molecules was shown to help in identifying T cell determinants. The specificity of DRB1*0401- restricted T cell hybridomas against human serum albumin corresponded to two peptides, predicted and shown to bind the class II protein with high affinity.
This article has been cited by other articles:
|
|
 |
|
  S. T. Chang, D. Ghosh, D. E. Kirschner, and J. J. Linderman Peptide length-based prediction of peptide-MHC class II binding Bioinformatics, November 15, 2006; 22(22): 2761 - 2767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Vrljic, S. Y. Nishimura, S. Brasselet, W. E. Moerner, and H. M. McConnell Translational Diffusion of Individual Class II MHC Membrane Proteins in Cells Biophys. J., November 1, 2002; 83(5): 2681 - 2692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Lippolis, F. M. White, J. A. Marto, C. J. Luckey, T. N. J. Bullock, J. Shabanowitz, D. F. Hunt, and V. H. Engelhard Analysis of MHC Class II Antigen Processing by Quantitation of Peptides that Constitute Nested Sets J. Immunol., November 1, 2002; 169(9): 5089 - 5097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Belmares, R. Busch, K. W. Wucherpfennig, H. M. McConnell, and E. D. Mellins Structural Factors Contributing to DM Susceptibility of MHC Class II/Peptide Complexes J. Immunol., November 1, 2002; 169(9): 5109 - 5117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. G. Forsthuber, C. L. Shive, W. Wienhold, K. de Graaf, E. G. Spack, R. Sublett, A. Melms, J. Kort, M. K. Racke, and R. Weissert T Cell Epitopes of Human Myelin Oligodendrocyte Glycoprotein Identified in HLA-DR4 (DRB1*0401) Transgenic Mice Are Encephalitogenic and Are Presented by Human B Cells J. Immunol., December 15, 2001; 167(12): 7119 - 7125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Novak, A. W. Liu, J. A. Gebe, B. A. Falk, G. T. Nepom, D. M. Koelle, and W. W. Kwok Tetramer-Guided Epitope Mapping: Rapid Identification and Characterization of Immunodominant CD4+ T Cell Epitopes from Complex Antigens J. Immunol., June 1, 2001; 166(11): 6665 - 6670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Texier, S. Pouvelle, M. Busson, M. Herve, D. Charron, A. Menez, and B. Maillere HLA-DR Restricted Peptide Candidates for Bee Venom Immunotherapy J. Immunol., March 15, 2000; 164(6): 3177 - 3184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. de Lalla, T. Sturniolo, L. Abbruzzese, J. Hammer, A. Sidoli, F. Sinigaglia, and P. Panina-Bordignon Cutting Edge: Identification of Novel T Cell Epitopes in Lol p5a by Computational Prediction J. Immunol., August 15, 1999; 163(4): 1725 - 1729. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Matsuo, L. Corlett, S. Hawke, M. Nicolle, P. Driscoll, S. Deshpande, E. Spack, and N. Willcox Distant interactions between dimorphisms in HLA-DR4 radically affect recognition of defined peptides by a specific T cell clone Int. Immunol., May 1, 1999; 11(5): 835 - 843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Gross, T. Forsthuber, M. Tary-Lehmann, C. Etling, K. Ito, Z. A. Nagy, J. A. Field, A. C. Steere, and B. T. Huber Identification of LFA-1 as a Candidate Autoantigen in Treatment-Resistant Lyme Arthritis Science, July 31, 1998; 281(5377): 703 - 706. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. C. Andersson, B. E. Hansen, H. Jacobsen, L. S. Madsen, C. B. Andersen, J. Engberg, J. B. Rothbard, G. S. McDevitt, V. Malmstrom, R. Holmdahl, et al. Definition of MHC and T cell receptor contacts in the HLA-DR4restricted immunodominant epitope in type II collagen and characterization of collagen-induced arthritis in HLA-DR4 and human CD4 transgenic mice PNAS, June 23, 1998; 95(13): 7574 - 7579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Phelps, V. L. Jones, M. Coughlan, A. N. Turner, and A. J. Rees Presentation of the Goodpasture Autoantigen to CD4 T Cells Is Influenced More by Processing Constraints Than by HLA Class II Peptide Binding Preferences J. Biol. Chem., May 8, 1998; 273(19): 11440 - 11447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Southwood, J. Sidney, A. Kondo, M.-F. del Guercio, E. Appella, S. Hoffman, R. T. Kubo, R. W. Chesnut, H. M. Grey, and A. Sette Several Common HLA-DR Types Share Largely Overlapping Peptide Binding Repertoires J. Immunol., April 1, 1998; 160(7): 3363 - 3373. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
