The Journal of Immunology, Vol 154, Issue 11 5620-5629, Copyright © 1995 by American Association of Immunologists

ARTICLES

Deletion of a C-terminal sequence of the class II-associated invariant chain abrogates invariant chains oligomer formation and class II antigen presentation

P Bertolino, M Staschewski, MC Trescol-Biemont, IM Freisewinkel, K Schenck, I Chretien, F Forquet, D Gerlier, C Rabourdin-Combe and N Koch
ENS-CNRS UMR 49, Lyon, France.

The MHC class II-associated invariant chain (Ii) is involved in Ag processing and presentation. Physical association of MHC class II molecules with Ii and an effect of Ii on peptide loading to class II have been demonstrated, but to date these functions have not been related to a particular region of Ii. We investigated luminal deletion mutants of Ii and their role in Ag processing and presentation. IAk- expressing L cells were transfected with deletion mutants of the Ii gene and assayed for their ability to present hen egg lysozyme to three different T cell hybridomas. It is shown that the sequence aa 131-191 of Ii is important for the presentation of native hen egg lysozyme. In addition, this C terminal region is shown to be responsible for Ii oligomer formation. It is therefore conceivable that oligomer formation of Ii is a prerequisite for class II-restricted Ag processing and presentation.

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