The Journal of Immunology, Vol 153, Issue 5 2161-2167, Copyright © 1994 by American Association of Immunologists

ARTICLES

Comparison of human eosinophil and neutrophil adhesion to endothelial cells under nonstatic conditions. Role of L-selectin

EF Knol, F Tackey, TF Tedder, DA Klunk, CA Bickel, SA Sterbinsky and BS Bochner
Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224.

To simulate adhesion that occurs under conditions of flow, we investigated the attachment of eosinophils to endothelium under rotational conditions. Tissue-culture plates containing monolayers of HUVEC were placed on a horizontal rotator (80 revolutions per minute (rpm)), and equal numbers of purified human eosinophils or neutrophils were added to separate wells at 4 degrees C. Binding of eosinophils and neutrophils to unstimulated endothelial cells was 15 +/- 3 and 31 +/- 11 cells/four high power fields (HPF), respectively. After preincubation of HUVEC with IL-1 beta (1 ng/ml, 4 h, 37 degrees C), adhesion increased to 56 +/- 4 and 290 +/- 26 cells/four HPF, respectively (p < 0.0002 for both, n = 8-14). Eosinophils with reduced levels of L-selectin (blood eosinophils activated in vitro or eosinophils obtained from bronchoalveolar lavage (BAL) performed after segmental lung allergen challenge of allergic subjects) demonstrated reduced binding under rotating conditions. Several L-selectin Abs inhibited adhesion of eosinophils and neutrophils (e.g., LAM1-3: 43 +/- 14% vs 63 +/- 3% inhibition; LAM1-6: 73 +/- 5% vs 36 +/- 6% inhibition, respectively, n > or = 6). Interestingly, one additional L-selectin Ab, LAM1-11, inhibited eosinophil but not neutrophil adhesion (51 +/- 2% vs 1 +/- 7% inhibition, respectively, n > or = 5). We conclude that eosinophils, like neutrophils, use L-selectin to bind to activated endothelial cells under conditions of flow, although mAb LAM1-11 can selectively inhibit eosinophil attachment to stimulated endothelial cells in vitro, suggesting different functional epitopes on L-selectin among eosinophils and neutrophils.

This article has been cited by other articles:

				O. J. T. McCarty, N. Tien, B. S. Bochner, and K. Konstantopoulos Exogenous eosinophil activation converts PSGL-1-dependent binding to CD18-dependent stable adhesion to platelets in shear flow Am J Physiol Cell Physiol, May 1, 2003; 284(5): C1223 - C1234. [Abstract] [Full Text] [PDF]

				L. H. Ulfman, D. P. H. Joosten, C. W. van Aalst, J.-W. J. Lammers, E. A. van de Graaf, L. Koenderman, and J. J. Zwaginga Platelets Promote Eosinophil Adhesion of Patients with Asthma to Endothelium under Flow Conditions Am. J. Respir. Cell Mol. Biol., April 1, 2003; 28(4): 512 - 519. [Abstract] [Full Text] [PDF]

				A. Burke-Gaffney, K. Blease, A. Hartnell, and P. G. Hellewell TNF-{alpha} Potentiates C5a-Stimulated Eosinophil Adhesion to Human Bronchial Epithelial Cells: A Role for {alpha}5{beta}1 Integrin J. Immunol., February 1, 2002; 168(3): 1380 - 1388. [Abstract] [Full Text] [PDF]

				B. S. Edwards, M. S. Curry, H. Tsuji, D. Brown, R. S. Larson, and L. A. Sklar Expression of P-Selectin at Low Site Density Promotes Selective Attachment of Eosinophils Over Neutrophils J. Immunol., July 1, 2000; 165(1): 404 - 410. [Abstract] [Full Text] [PDF]

				P. Sriramarao, R. G. DiScipio, R. R. Cobb, M. Cybulsky, G. Stachnick, D. Castaneda, M. Elices, and D. H. Broide VCAM-1 is more effective than MAdCAM-1 in supporting eosinophil rolling under conditions of shear flow Blood, January 15, 2000; 95(2): 592 - 601. [Abstract] [Full Text] [PDF]

				S. D. Robinson, P. S. Frenette, H. Rayburn, M. Cummiskey, M. Ullman-Cullere, D. D. Wagner, and R. O. Hynes Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment PNAS, September 28, 1999; 96(20): 11452 - 11457. [Abstract] [Full Text] [PDF]

				M. A. Jagels, P. J. Daffern, B. L. Zuraw, and T. E. Hugli Mechanisms and Regulation of Polymorphonuclear Leukocyte and Eosinophil Adherence to Human Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., September 1, 1999; 21(3): 418 - 427. [Abstract] [Full Text]

				L. H. Ulfman, P. H. M. Kuijper, J. A. M. van der Linden, J.-W. J. Lammers, J. J. Zwaginga, and L. Koenderman Characterization of Eosinophil Adhesion to TNF-{alpha}-Activated Endothelium Under Flow Conditions: {alpha}4 Integrins Mediate Initial Attachment, and E-Selectin Mediates Rolling J. Immunol., July 1, 1999; 163(1): 343 - 350. [Abstract] [Full Text] [PDF]

				M. A. Giembycz and M. A. Lindsay Pharmacology of the Eosinophil Pharmacol. Rev., June 1, 1999; 51(2): 213 - 340. [Abstract] [Full Text] [PDF]

				K. D. Patel Mechanisms of Selective Leukocyte Recruitment from Whole Blood on Cytokine-Activated Endothelial Cells Under Flow Conditions J. Immunol., May 15, 1999; 162(10): 6209 - 6216. [Abstract] [Full Text] [PDF]

				H. Sakurai, F. C. Schmalstieg, L. D. Traber, H. K. Hawkins, and D. L. Traber Role of L-selectin in physiological manifestations after burn and smoke inhalation injury in sheep J Appl Physiol, April 1, 1999; 86(4): 1151 - 1159. [Abstract] [Full Text] [PDF]

				R. G. DiScipio, P. J. Daffern, M. A. Jagels, D. H. Broide, and P. Sriramarao A Comparison of C3a and C5a-Mediated Stable Adhesion of Rolling Eosinophils in Postcapillary Venules and Transendothelial Migration In Vitro and In Vivo J. Immunol., January 15, 1999; 162(2): 1127 - 1136. [Abstract] [Full Text] [PDF]

				M.-K. Kim, B. K. Brandley, M. B. Anderson, and B. S. Bochner Antagonism of Selectin-Dependent Adhesion of Human Eosinophils and Neutrophils by Glycomimetics and Oligosaccharide Compounds Am. J. Respir. Cell Mol. Biol., November 1, 1998; 19(5): 836 - 841. [Abstract] [Full Text]

				H.-U. Simon, S. Yousefi, B. Dibbert, H. Hebestreit, M. Weber, D. R. Branch, K. Blaser, F. Levi-Schaffer, and G. P. Anderson Role for Tyrosine Phosphorylation and Lyn Tyrosine Kinase in Fas Receptor-Mediated Apoptosis in Eosinophils Blood, July 15, 1998; 92(2): 547 - 557. [Abstract] [Full Text] [PDF]

				D. H. Broide, D. Humber, and P. Sriramarao Inhibition of Eosinophil Rolling and Recruitment in P-Selectin- and Intracellular Adhesion Molecule-1-Deficient Mice Blood, April 15, 1998; 91(8): 2847 - 2856. [Abstract] [Full Text] [PDF]