The Journal of Immunology, Vol 153, Issue 2 637-646, Copyright © 1994 by American Association of Immunologists

ARTICLES

V beta repertoire of murine hepatic T cells. Implication for selection of double negative alpha beta + T cells

S Seki, DH Kono, RS Balderas and AN Theofilopoulos
Scripps Research Institute, Department of Immunology, La Jolla, California 92037.

To further define the origin, selection, and diversity of hepatic T cells, we have determined V beta gene expression profiles in double negative (DN, CD4-8-) and single positive (SP, CD4+8- or CD8+4-) alpha beta + liver T cells of DBA/2 mice. These I-E+ mice express mouse mammary tumor (Mtv) provirus-encoded endogenous superantigens of the Mlsa,c type, and thus display deletions/depletions of several V beta- bearing SP cells. Total liver alpha beta + T cells of these mice exhibited an overall V beta expression profile similar to splenic T cells, with the notable exception of high V beta 7 and V beta 8.1 expression. As previously reported, DN alpha beta + T cells were enriched highly in the liver. This subset exhibited a V beta expression profile similar to thymic DN alpha beta + cells with deletions/depletions in several V beta s, but high V beta 7 expression in both populations. Surprisingly, hepatic CD4+ cells also displayed high V beta 7 expression compared with splenic T cells, suggesting that hepatic DN alpha beta + and CD4+ T cells are selected via a common pathway. The V beta 7-expressing DN alpha beta + and CD4+ liver T cell populations were polyclonal, as evidenced by cloning and sequencing. High V beta 7 expression in these cells was undiminished with age. On the basis of V beta repertoire and surface phenotype, DN alpha beta + and/or certain CD4+ T cells seem to constitute a distinct population primarily found in the liver, thymus, and bone marrow. These cells may originate from SP T cells that have down-regulated their accessory molecules under certain activation conditions and, because of the accompanying expression of particular adhesion molecules, they accumulate in tissues such as the liver and thymus.

This article has been cited by other articles:

				D. B. Ascon, M. Ascon, S. Satpute, S. Lopez-Briones, L. Racusen, R. B. Colvin, M. J. Soloski, and H. Rabb Normal mouse kidneys contain activated and CD3+CD4-CD8- double-negative T lymphocytes with a distinct TCR repertoire J. Leukoc. Biol., December 1, 2008; 84(6): 1400 - 1409. [Abstract] [Full Text] [PDF]

				R. Nakagawa, T. Inui, I. Nagafune, Y. Tazunoki, K. Motoki, A. Yamauchi, M. Hirashima, Y. Habu, H. Nakashima, and S. Seki Essential Role of Bystander Cytotoxic CD122+CD8+ T Cells for the Antitumor Immunity Induced in the Liver of Mice by {alpha}-Galactosylceramide J. Immunol., June 1, 2004; 172(11): 6550 - 6557. [Abstract] [Full Text] [PDF]

				T. Inui, R. Nakagawa, S. Ohkura, Y. Habu, Y. Koike, K. Motoki, N. Kuranaga, M. Fukasawa, N. Shinomiya, and S. Seki Age-Associated Augmentation of the Synthetic Ligand- Mediated Function of Mouse NK1.1 Ag+ T Cells: Their Cytokine Production and Hepatotoxicity In Vivo and In Vitro J. Immunol., December 1, 2002; 169(11): 6127 - 6132. [Abstract] [Full Text] [PDF]

				R. Nakagawa, I. Nagafune, Y. Tazunoki, H. Ehara, H. Tomura, R. Iijima, K. Motoki, M. Kamishohara, and S. Seki Mechanisms of the Antimetastatic Effect in the Liver and of the Hepatocyte Injury Induced by {{alpha}}-Galactosylceramide in Mice J. Immunol., June 1, 2001; 166(11): 6578 - 6584. [Abstract] [Full Text] [PDF]

				Y. Habu, S. Seki, E. Takayama, T. Ohkawa, Y. Koike, K. Ami, T. Majima, and H. Hiraide The Mechanism of a Defective IFN-{{gamma}} Response to Bacterial Toxins in an Atopic Dermatitis Model, NC/Nga Mice, and the Therapeutic Effect of IFN-{{gamma}}, IL-12, or IL-18 on Dermatitis J. Immunol., May 1, 2001; 166(9): 5439 - 5447. [Abstract] [Full Text] [PDF]

				K. Ogasawara, K. Takeda, W. Hashimoto, M. Satoh, R. Okuyama, N. Yanai, M. Obinata, K. Kumagai, H. Takada, H. Hiraide, et al. Involvement of NK1+ T Cells and Their IFN-{gamma} Production in the Generalized Shwartzman Reaction J. Immunol., April 1, 1998; 160(7): 3522 - 3527. [Abstract] [Full Text] [PDF]

				O. Lantz, L. I. Sharara, F. Tilloy, A. Andersson, and J. P. DiSanto Lineage Relationships and Differentiation of Natural Killer (NK) T Cells: Intrathymic Selection and Interleukin (IL)-4 Production in the Absence of NKR-P1 and Ly49 Molecules J. Exp. Med., April 21, 1997; 185(8): 1395 - 1402. [Abstract] [Full Text] [PDF]