| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 153, Issue 11 4934-4939, Copyright © 1994 by American Association of Immunologists
ARTICLES |
S Hayakawa, S Saito, N Nemoto, F Chishima, K Akiyama, H Shiraishi, J Hayakawa, M Karasaki-Suzuki, KT Fujii and M Ichijo
Department of Obstetrics and Gynaecology, Nihon University School of Medicine, Japan.
Recombinase-activating genes (RAG-1 and RAG-2) are expressed in immature T or B lymphocytes and possess activity to induce V(D)J rearrangement in TCR and Ig genes. We examined their expression in human decidual and non-pregnant endometrial samples using a highly sensitive RT-PCR technique. Expression of RAG-1 and 2 was noted in all (13/13) pregnant decidual tissues obtained at different gestational stages. After anti-human Ig treatment to rule out the possibility of RAG-1,2 expression from decidual B-cells, strong expression of RAGs was still noted in B cell depleted decidual cells in contrast to lymph nodes and PBMC that lost RAG mRNA expression after this treatment. After FACS mediated cell sorting, strong expression of RAG-1,2 was noted in CD16-CD56bright cells and weak expression in CD3+ cells. Although CD16-CD56bright cells lack surface CD3, they express CD3 epsilon mRNA only detectable by RT-PCR. Our results suggest the nature of decidual CD16-CD56bright cells as a progenitor of extrathymic T cells that possess RAG-1 and 2 mRNA as markers of their immaturity, and possibly differentiate into CD3+ extrathymic T-cells in the decidua under the influence of trophoblastic cells. We propose the human decidua as a new site of extrathymic T-cells differentiation and propose possible roles of trophoblastic cells to attract progenitor lymphocytes of bone marrow origin and trigger their TCR rearrangement as thymic epithelial cells.
This article has been cited by other articles:
|
|
 |
|
  L. Lynch, L. Golden-Mason, M. Eogan, C. O'Herlihy, and C. O'Farrelly Cells with haematopoietic stem cell phenotype in adult human endometrium: relevance to infertility? Hum. Reprod., April 1, 2007; 22(4): 919 - 926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Williams, P. W. Bland, A. C. Phillips, S. Turner, T. Brooklyn, G. Shaya, R. D. Spicer, and C. S. J. Probert Intestinal {alpha}{beta} T Cells Differentiate and Rearrange Antigen Receptor Genes In Situ in the Human Infant J. Immunol., December 15, 2004; 173(12): 7190 - 7199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Redline Role of Uterine Natural Killer Cells and Interferon {gamma} in Placental Development J. Exp. Med., July 18, 2000; 192(2): F1 - F4. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Searle, R. K. Jones, and J. N. Bulmer Phenotypic Analysis and Proliferative Responses of Human Endometrial Granulated Lymphocytes during the Menstrual Cycle Biol Reprod, April 1, 1999; 60(4): 871 - 878. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Reue, R. D. Cohen, and M. C. Schotz Evidence for Hormone-Sensitive Lipase mRNA Expression in Human Monocyte/Macrophages Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3428 - 3432. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Cunnane, C. O'Farrelly, C. A. Michie, A. R. McLean, L. W. Moreland, R. P. Bucy, W. J. Koopman, C. L. Mackall, S. M. Steinberg, and R. E. Gress Regeneration of T Cells after Chemotherapy N. Engl. J. Med., June 15, 1995; 332(24): 1650 - 1652. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
