The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Harding, C. V.
Right arrow Articles by Song, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harding, C. V.
Right arrow Articles by Song, R.

The Journal of Immunology, Vol 153, Issue 11 4925-4933, Copyright © 1994 by American Association of Immunologists

ARTICLES

Phagocytic processing of exogenous particulate antigens by macrophages for presentation by class I MHC molecules

CV Harding and R Song
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.

Exogenous Ags that are processed in vacuolar endocytic compartments are generally presented by class II MHC molecules and not class I MHC (MHC- I) molecules, which conventionally present cytoplasmic or endogenous Ags. Accordingly, i.v. immunization of C57BL/6 mice with soluble OVA did not elicit a CD8 T cell response. However, i.v. immunization with OVA coupled to Latex particles (Latex-OVA) elicited an OVA-specific CD8 T cell response in vivo (particles from 59 to 2000 nm diameter were effective). In vitro, Latex-OVA was processed by H-2b macrophages and presented by Kb at least 100- to 1000-fold more efficiently than was soluble OVA. Inhibition of phagocytosis by cytochalasin D blocked the processing of Latex-OVA, whereas processing was not blocked by Brefeldin A. Latex-OVA was presented directly by H-2b macrophages or after "regurgitation" of processed OVA peptide from viable MHC- disparate macrophages for binding to surface Kb molecules on fixed H-2b macrophages. Peptide regurgitation was observed during processing of both Latex-OVA and Salmonella typhimurium 14028s that express an OVA fusion protein (Crl-OVA). However, the regurgitation pathway was less efficient than direct processing by viable H-2b macrophages. Thus, macrophages express an alternate pathway that allows MHC-I presentation of vacuolar exogenous particulate Ags, including inert synthetic particles without lipid membranes and intravacuolar bacteria. Peptides from these Ags are released from intracellular compartments to bind to surface MHC-I molecules, but peptide-MHC-I complexes also may be generated within intracellular compartments.


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
I. M. Olazabal, N. B. Martin-Cofreces, M. Mittelbrunn, G. Martinez del Hoyo, B. Alarcon, and F. Sanchez-Madrid
Activation Outcomes Induced in Naive CD8 T-Cells by Macrophages Primed via "Phagocytic" and Nonphagocytic Pathways
Mol. Biol. Cell, February 1, 2008; 19(2): 701 - 710.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
D. Accapezzato, V. Visco, V. Francavilla, C. Molette, T. Donato, M. Paroli, M. U. Mondelli, M. Doria, M. R. Torrisi, and V. Barnaba
Chloroquine enhances human CD8+ T cell responses against soluble antigens in vivo
J. Exp. Med., September 19, 2005; 202(6): 817 - 828.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. Rosales-Reyes, C. Alpuche-Aranda, M. d. l. L. Ramirez-Aguilar, A. D. Castro-Eguiluz, and V. Ortiz-Navarrete
Survival of Salmonella enterica Serovar Typhimurium within Late Endosomal-Lysosomal Compartments of B Lymphocytes Is Associated with the Inability To Use the Vacuolar Alternative Major Histocompatibility Complex Class I Antigen-Processing Pathway
Infect. Immun., July 1, 2005; 73(7): 3937 - 3944.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. H. Ringrose, H. D. Meiring, D. Speijer, T. E. W. Feltkamp, C. A. C. M. van Els, A. P. J. M. de Jong, and J. Dankert
Major Histocompatibility Complex Class I Peptide Presentation after Salmonella enterica Serovar Typhimurium Infection Assessed via Stable Isotope Tagging of the B27-Presented Peptide Repertoire
Infect. Immun., September 1, 2004; 72(9): 5097 - 5105.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Dani, A. Chaudhry, P. Mukherjee, D. Rajagopal, S. Bhatia, A. George, V. Bal, S. Rath, and S. Mayor
The pathway for MHCII-mediated presentation of endogenous proteins involves peptide transport to the endo-lysosomal compartment
J. Cell Sci., August 15, 2004; 117(18): 4219 - 4230.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. R. Tvinnereim, S. E. Hamilton, and J. T. Harty
Neutrophil Involvement in Cross-Priming CD8+ T Cell Responses to Bacterial Antigens
J. Immunol., August 1, 2004; 173(3): 1994 - 2002.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
W.-F. Lo, C. D. Dunn, H. Ong, E. S. Metcalf, and M. J. Soloski
Bacterial and Host Factors Involved in the Major Histocompatibility Complex Class Ib-Restricted Presentation of Salmonella Hsp 60: Novel Pathway
Infect. Immun., May 1, 2004; 72(5): 2843 - 2849.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
M. Giri, K. E. Ugen, and D. B. Weiner
DNA Vaccines against Human Immunodeficiency Virus Type 1 in the Past Decade
Clin. Microbiol. Rev., April 1, 2004; 17(2): 370 - 389.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. F. Fonteneau, D. G. Kavanagh, M. Lirvall, C. Sanders, T. L. Cover, N. Bhardwaj, and M. Larsson
Characterization of the MHC class I cross-presentation pathway for cell-associated antigens by human dendritic cells
Blood, December 15, 2003; 102(13): 4448 - 4455.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. E. Skelsey, E. Mayhew, and J. Y. Niederkorn
Splenic B Cells Act as Antigen Presenting Cells for the Induction of Anterior Chamber-Associated Immune Deviation
Invest. Ophthalmol. Vis. Sci., December 1, 2003; 44(12): 5242 - 5251.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Luhrs, W. Schmidt, R. Kutil, M. Buschle, S. N. Wagner, G. Stingl, and A. Schneeberger
Induction of Specific Immune Responses by Polycation-Based Vaccines
J. Immunol., November 1, 2002; 169(9): 5217 - 5226.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
X. Shen, S. B. J. Wong, C. B. Buck, J. Zhang, and R. F. Siliciano
Direct Priming and Cross-Priming Contribute Differentially to the Induction of CD8+ CTL Following Exposure to Vaccinia Virus Via Different Routes
J. Immunol., October 15, 2002; 169(8): 4222 - 4229.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Alexander, C. Oseroff, C. Dahlberg, M. Qin, G. Ishioka, M. Beebe, J. Fikes, M. Newman, R. W. Chesnut, P. A. Morton, et al.
A Decaepitope Polypeptide Primes for Multiple CD8+ IFN-{gamma} and Th Lymphocyte Responses: Evaluation of Multiepitope Polypeptides as a Mode for Vaccine Delivery
J. Immunol., June 15, 2002; 168(12): 6189 - 6198.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. G. Chikh, S. Kong, M. B. Bally, J.-C. Meunier, and M.-P. M. Schutze-Redelmeier
Efficient Delivery of Antennapedia Homeodomain Fused to CTL Epitope with Liposomes into Dendritic Cells Results in the Activation of CD8+ T Cells
J. Immunol., December 1, 2001; 167(11): 6462 - 6470.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
A. Fortin, J. Lagace, and H.-M. Therien
Trafficking of Surface-linked and Encapsulated Liposomal Antigens in Macrophages: an Immunocytochemical Study
J. Histochem. Cytochem., November 1, 2001; 49(11): 1407 - 1420.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
I. Popov, C. S. Dela Cruz, B. H. Barber, B. Chiu, and R. D. Inman
The Effect of an Anti-HLA-B27 Immune Response on CTL Recognition of Chlamydia
J. Immunol., September 15, 2001; 167(6): 3375 - 3382.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Chun, A. G. Grandea III, L. Lybarger, J. Forman, L. Van Kaer, and C.-R. Wang
Functional Roles of TAP and Tapasin in the Assembly of M3-N-Formylated Peptide Complexes
J. Immunol., August 1, 2001; 167(3): 1507 - 1514.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. T. Maecker, S. A. Ghanekar, M. A. Suni, X.-S. He, L. J. Picker, and V. C. Maino
Factors Affecting the Efficiency of CD8+ T Cell Cross-Priming with Exogenous Antigens
J. Immunol., June 15, 2001; 166(12): 7268 - 7275.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Chan, D. J. Lee, A. Schubert, C. M. Tang, B. Crain, S. P. Schoenberger, and M. Corr
The Roles of MHC Class II, CD40, and B7 Costimulation in CTL Induction by Plasmid DNA
J. Immunol., March 1, 2001; 166(5): 3061 - 3066.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
O. Neyrolles, K. Gould, M.-P. Gares, S. Brett, R. Janssen, P. O'Gaora, J.-L. Herrmann, M.-C. Prevost, E. Perret, J. E. R. Thole, et al.
Lipoprotein Access to MHC Class I Presentation During Infection of Murine Macrophages with Live Mycobacteria
J. Immunol., January 1, 2001; 166(1): 447 - 457.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. P. Fischbein, A. Ardehali, J. Yun, S. Schoenberger, H. Laks, Y. Irie, P. Dempsey, G. Cheng, M. C. Fishbein, and B. Bonavida
CD40 Signaling Replaces CD4+ Lymphocytes and Its Blocking Prevents Chronic Rejection of Heart Transplants
J. Immunol., December 15, 2000; 165(12): 7316 - 7322.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
G. Ferlazzo, C. Semino, G. M. Spaggiari, M. Meta, M. C. Mingari, and G. Melioli
Dendritic cells efficiently cross-prime HLA class I-restricted cytolytic T lymphocytes when pulsed with both apoptotic and necrotic cells but not with soluble cell-derived lysates
Int. Immunol., December 1, 2000; 12(12): 1741 - 1747.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
L. J. Sigal and K. L. Rock
Bone Marrow-derived Antigen-presenting Cells Are Required for the Generation of Cytotoxic T Lymphocyte Responses to Viruses and Use Transporter Associated with Antigen Presentation (TAP)-dependent and -independent Pathways of Antigen Presentation
J. Exp. Med., October 16, 2000; 192(8): 1143 - 1150.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Haicheur, E. Bismuth, S. Bosset, O. Adotevi, G. Warnier, V. Lacabanne, A. Regnault, C. Desaymard, S. Amigorena, P. Ricciardi-Castagnoli, et al.
The B Subunit of Shiga Toxin Fused to a Tumor Antigen Elicits CTL and Targets Dendritic Cells to Allow MHC Class I-Restricted Presentation of Peptides Derived from Exogenous Antigens
J. Immunol., September 15, 2000; 165(6): 3301 - 3308.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. P. Peppelenbosch, M. DeSmedt, G. Pynaert, S. J. H. van Deventer, and J. Grooten
Macrophages Present Pinocytosed Exogenous Antigen Via MHC Class I Whereas Antigen Ingested by Receptor-Mediated Endocytosis Is Presented Via MHC Class II
J. Immunol., August 15, 2000; 165(4): 1984 - 1991.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. Sedlik, G. Dadaglio, M. F. Saron, E. Deriaud, M. Rojas, S. I. Casal, and C. Leclerc
In Vivo Induction of a High-Avidity, High-Frequency Cytotoxic T-Lymphocyte Response Is Associated with Antiviral Protective Immunity
J. Virol., July 1, 2000; 74(13): 5769 - 5775.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
A. R. Tvinnereim and J. T. Harty
CD8+ T-Cell Priming against a Nonsecreted Listeria monocytogenes Antigen Is Independent of the Antimicrobial Activities of Gamma Interferon
Infect. Immun., April 1, 2000; 68(4): 2196 - 2204.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. J. Kurlander, E. Chao, J. Fields, and C. Nataraj
The Adjacent Flanking Region Plays a Critical Role in Facilitating the Presentation of the Listeria monocytogenes Product lemA to H2 M3wt-Restricted, Peptide-Specific Murine CD8 Cells
J. Immunol., December 15, 1999; 163(12): 6741 - 6747.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Corr, A. von Damm, D. J. Lee, and H. Tighe
In Vivo Priming by DNA Injection Occurs Predominantly by Antigen Transfer
J. Immunol., November 1, 1999; 163(9): 4721 - 4727.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. M. Doling, J. D. Ballard, H. Shen, K. M. Krishna, R. Ahmed, R. J. Collier, and M. N. Starnbach
Cytotoxic T-Lymphocyte Epitopes Fused to Anthrax Toxin Induce Protective Antiviral Immunity
Infect. Immun., July 1, 1999; 67(7): 3290 - 3296.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W.-F. Lo, H. Ong, E. S. Metcalf, and M. J. Soloski
T Cell Responses to Gram-Negative Intracellular Bacterial Pathogens: A Role for CD8+ T Cells in Immunity to Salmonella Infection and the Involvement of MHC Class Ib Molecules
J. Immunol., May 1, 1999; 162(9): 5398 - 5406.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Bansal, P. Mukherjee, S. K. Basu, A. George, V. Bal, and S. Rath
MHC Class I-Restricted Presentation of Maleylated Protein Binding to Scavenger Receptors
J. Immunol., April 15, 1999; 162(8): 4430 - 4437.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
E. Liebana, R. M. Girvin, M. Welsh, S. D. Neill, and J. M. Pollock
Generation of CD8+ T-Cell Responses to Mycobacterium bovis and Mycobacterial Antigen in Experimental Bovine Tuberculosis
Infect. Immun., March 1, 1999; 67(3): 1034 - 1044.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
O. Denis and K. Huygen
Characterization of the culture filtrate-specific cytotoxic T lymphocyte response induced by bacillus Calmette-Guerin vaccination in H-2b mice
Int. Immunol., February 1, 1999; 11(2): 209 - 216.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
K. A. Foon, J. Yannelli, and M. Bhattacharya-Chatterjee
Colorectal Cancer as a Model for Immunotherapy
Clin. Cancer Res., February 1, 1999; 5(2): 225 - 236.
[Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
U. Zugel and S. H. E. Kaufmann
Role of Heat Shock Proteins in Protection from and Pathogenesis of Infectious Diseases
Clin. Microbiol. Rev., January 1, 1999; 12(1): 19 - 39.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Brewer, L. Tetley, J. Richmond, F. Y. Liew, and J. Alexander
Lipid Vesicle Size Determines the Th1 or Th2 Response to Entrapped Antigen
J. Immunol., October 15, 1998; 161(8): 4000 - 4007.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. J. Sigal, H. Reiser, and K. L. Rock
The Role of B7-1 and B7-2 Costimulation for the Generation of CTL Responses In Vivo
J. Immunol., September 15, 1998; 161(6): 2740 - 2745.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. Mark Tompkins, J. R. Kraft, C. T. Dao, M. J. Soloski, and P. E. Jensen
Transporters Associated with Antigen Processing (TAP)-independent Presentation of Soluble Insulin to alpha /beta T Cells by the Class Ib Gene Product, Qa-1b
J. Exp. Med., September 7, 1998; 188(5): 961 - 971.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Bonnotte, N. Favre, M. Moutet, A. Fromentin, E. Solary, M. Martin, and F. Martin
Bcl-2-Mediated Inhibition of Apoptosis Prevents Immunogenicity and Restores Tumorigenicity of Spontaneously Regressive Tumors
J. Immunol., August 1, 1998; 161(3): 1433 - 1438.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. L. Richards, M. Rao, N. M. Wassef, G. M. Glenn, S. W. Rothwell, and C. R. Alving
Liposomes Containing Lipid A Serve as an Adjuvant for Induction of Antibody and Cytotoxic T-Cell Responses against RTS,S Malaria Antigen
Infect. Immun., June 1, 1998; 66(6): 2859 - 2865.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. N. Balaji and W. H. Boom
Processing of Mycobacterium tuberculosis Bacilli by Human Monocytes for CD4+ alpha beta and gamma delta T Cells: Role of Particulate Antigen
Infect. Immun., January 1, 1998; 66(1): 98 - 106.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Tjelle, B Saigal, M Froystad, and T Berg
Degradation of phagosomal components in late endocytic organelles
J. Cell Sci., January 1, 1998; 111(1): 141 - 148.
[Abstract] [PDF]

Home page
 The OncologistHome page
S. Osanto
Vaccine Trials for the Clinician: Prospects for Tumor Antigens
Oncologist, October 1, 1997; 2(5): 284 - 299.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1994 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1994 by The American Association of Immunologists, Inc. All rights reserved.