| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 152, Issue 9 4612-4617, Copyright © 1994 by American Association of Immunologists
ARTICLES |
P Renesto, L Halbwachs-Mecarelli, P Nusbaum, P Lesavre and M Chignard
Cell Pharmacology Unit, Associated Unit IP/INSERM 285, Pasteur Institute, Paris, France.
Purified proteinase 3 (PR3) devoid of any elastase (HLE) and cathepsin G (Cat.G) contaminants, was prepared from azurophilic granules of human polymorphonuclear neutrophils by using a novel procedure. Although unable to induce platelet activation (up to 25 micrograms/ml) by itself, PR3 at a concentration as low as 2.5 micrograms/ml enhanced the platelet response to a concomitantly added threshold concentration of Cat.G, a recognized platelet agonist. In the presence of 10 micrograms/ml PR3, aggregation and degranulation of platelets induced by Cat.G were 43.2 +/- 5.9% and 27.1 +/- 1.9% as compared with 5.5 +/- 2.9% and 4.2 +/- 1.5% (n = 4) for Cat.G alone. This enhancing effect by PR3 was also observed with collagen and a cyclic endoperoxide analogue, and was inhibited by eglin C and elafin, two PR3 inhibitors. Associated with the removal of activity by a anti-PR3 mAb and the lack of effect of the secretory leukocyte proteinase inhibitor, these data demonstrated that the effect is specifically related to the enzymatic activity of PR3. It is hypothesized that this mechanism could play a role in the polymorphonuclear neutrophil-mediated platelet activation, an event already known to be dependent on Cat.G and HLE. This is supported by the fact that the association of PR3 and HLE, at concentrations ineffective by themselves, was able to potentiate Cat.G- induced platelet activation.
This article has been cited by other articles:
|
|
 |
|
  A. Guillabert, V. Wittamer, B. Bondue, V. Godot, V. Imbault, M. Parmentier, and D. Communi Role of neutrophil proteinase 3 and mast cell chymase in chemerin proteolytic regulation J. Leukoc. Biol., December 1, 2008; 84(6): 1530 - 1538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mambole, D. Baruch, P. Nusbaum, S. Bigot, M. Suzuki, P. Lesavre, M. Fukuda, and L. Halbwachs-Mecarelli The Cleavage of Neutrophil Leukosialin (CD43) by Cathepsin G Releases Its Extracellular Domain and Triggers Its Intramembrane Proteolysis by Presenilin/{gamma}-Secretase J. Biol. Chem., August 29, 2008; 283(35): 23627 - 23635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bauer, M. Abdgawad, L. Gunnarsson, M. Segelmark, H. Tapper, and T. Hellmark Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils J. Leukoc. Biol., February 1, 2007; 81(2): 458 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Specks, D. N. Fass, J. D. Finkielman, A. M. Hummel, M. A. Viss, R. D. Litwiller, and C. J. Mcdonald Functional Significance of Asn-linked Glycosylation of Proteinase 3 for Enzymatic Activity, Processing, Targeting, and Recognition by Anti-neutrophil Cytoplasmic Antibodies J. Biochem., January 1, 2007; 141(1): 101 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-A. Park, F. He, L. D. Martin, Y. Li, B. N. Chorley, and K. B. Adler Human Neutrophil Elastase Induces Hypersecretion of Mucin from Well-Differentiated Human Bronchial Epithelial Cells in Vitro via a Protein Kinase C{delta}-Mediated Mechanism Am. J. Pathol., September 1, 2005; 167(3): 651 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sugawara, A. Uehara, T. Nochi, T. Yamaguchi, H. Ueda, A. Sugiyama, K. Hanzawa, K. Kumagai, H. Okamura, and H. Takada Neutrophil Proteinase 3-Mediated Induction of Bioactive IL-18 Secretion by Human Oral Epithelial Cells J. Immunol., December 1, 2001; 167(11): 6568 - 6575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. van der Geld, P. C. Limburg, and C. G. M. Kallenberg Proteinase 3, Wegener's autoantigen: from gene to antigen J. Leukoc. Biol., February 1, 2001; 69(2): 177 - 190. [Abstract] [Full Text]
|
|
|
|
|
|
S. Kurosawa, C. T. Esmon, and D. J. Stearns-Kurosawa The Soluble Endothelial Protein C Receptor Binds to Activated Neutrophils: Involvement of Proteinase-3 and CD11b/CD18 J. Immunol., October 15, 2000; 165(8): 4697 - 4703. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Witko-Sarsat, E. M. Cramer, C. Hieblot, J. Guichard, P. Nusbaum, S. Lopez, P. Lesavre, and L. Halbwachs-Mecarelli Presence of Proteinase 3 in Secretory Vesicles: Evidence of a Novel, Highly Mobilizable Intracellular Pool Distinct From Azurophil Granules Blood, October 1, 1999; 94(7): 2487 - 2496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Witko-Sarsat, L. Halbwachs-Mecarelli, A. Schuster, P. Nusbaum, I. Ueki, S. Canteloup, G. Lenoir, B. Descamps-Latscha, and J. A. Nadel Proteinase 3, a Potent Secretagogue in Airways, Is Present in Cystic Fibrosis Sputum Am. J. Respir. Cell Mol. Biol., April 1, 1999; 20(4): 729 - 736. [Abstract] [Full Text]
|
|
|
|
|
|
F. Tacchini-Cottier, C. Vesin, M. Redard, W. Buurman, and P. F. Piguet Role of TNFR1 and TNFR2 in TNF-Induced Platelet Consumption in Mice J. Immunol., June 15, 1998; 160(12): 6182 - 6186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Robache-Gallea, V.ér. Morand, J. M. Bruneau, B. Schoot, E. Tagat, E. Réalo, S. Chouaib, and S. Roman-Roman In Vitro Processing of Human Tumor Necrosis Factor-alpha J. Biol. Chem., October 6, 1995; 270(40): 23688 - 23692. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
