| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 152, Issue 6 3006-3016, Copyright © 1994 by American Association of Immunologists
ARTICLES |
D MacGlashan Jr, JM White, SK Huang, SJ Ono, JT Schroeder and LM Lichtenstein
Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224.
Purified human basophils have been examined for secretion of IL-4 protein and expression of IL-4 mRNA after stimulation with several secretagogues. In general, these studies used a 15-min preincubation with IL-3, before challenge with secretagogues. Under these conditions, IL-4 release averaged 30 pg/10(6) basophils (range 4-70) after challenge with anti-IgE Ab. FMLP and C5a led to somewhat lower levels of secretion. A direct comparison of basophils at 88 to 99% purity with basophils from the same preparations, but at lower purities, showed that the amount of IL-4 secretion was proportional to the purity of the basophils. The presence of mRNA for IL-4 (as determined by reverse transcriptase-PCR, competitive reverse transcriptase-PCR, or Northern blots) was also strictly related to the purity of the basophils. IL-4 mRNA was also found to be constitutively present and was increased after stimulation. The concentration of polyclonal anti-IgE Ab required for optimal IL-4 release was somewhat less than that required for optimal histamine release. IL-4 secretion was slower (t1/2 of 1.5 h) than histamine release and was inhibited by cycloheximide. In a final series of studies, we found that IL-3 was not required for IL-4 secretion; a short 15-min preincubation with IL-3 resulted in the same or slightly less IL-4 release than no treatment with IL-3. In contrast, an 18-h pretreatment with IL-3 resulted in a nearly tenfold increase in IL-4 secretion. We conclude that human basophils secrete IL-4 in response to several secretagogues and that IL-3 priming is not necessary to observe IL-4 secretion.
This article has been cited by other articles:
|
|
 |
|
  Z. Yuan, R. Wang, Y. Lee, C. Y. Chen, X. Yu, Z. Wu, D. Huang, L. Shen, and Z. W. Chen Tuberculosis-Induced Variant IL-4 mRNA Encodes a Cytokine Functioning As Growth Factor for (E)-4-Hydroxy-3-Methyl-But-2-Enyl Pyrophosphate-Specific V{gamma}2V{delta}2 T Cells J. Immunol., January 15, 2009; 182(2): 811 - 819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. MacGlashan Jr., S. Ishmael, S. M. MacDonald, J. M. Langdon, J. P. Arm, and D. E. Sloane Induced Loss of Syk in Human Basophils by Non-IgE-Dependent Stimuli J. Immunol., March 15, 2008; 180(6): 4208 - 4217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. MacGlashan Jr. Endocytosis, recycling, and degradation of unoccupied Fc{epsilon}RI in human basophils J. Leukoc. Biol., October 1, 2007; 82(4): 1003 - 1010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. de Paulis, N. Prevete, I. Fiorentino, F. W. Rossi, S. Staibano, N. Montuori, P. Ragno, A. Longobardi, B. Liccardo, A. Genovese, et al. Expression and Functions of the Vascular Endothelial Growth Factors and Their Receptors in Human Basophils J. Immunol., November 15, 2006; 177(10): 7322 - 7331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. MacGlashan Jr. and N. Vilarino Nonspecific Desensitization, Functional Memory, and the Characteristics of SHIP Phosphorylation following IgE-Mediated Stimulation of Human Basophils J. Immunol., July 15, 2006; 177(2): 1040 - 1051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Voehringer, T. A. Reese, X. Huang, K. Shinkai, and R. M. Locksley Type 2 immunity is controlled by IL-4/IL-13 expression in hematopoietic non-eosinophil cells of the innate immune system J. Exp. Med., June 12, 2006; 203(6): 1435 - 1446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hida, M. Tadachi, T. Saito, and S. Taki Negative control of basophil expansion by IRF-2 critical for the regulation of Th1/Th2 balance Blood, September 15, 2005; 106(6): 2011 - 2017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Vilarino, K. Miura, and D. W. MacGlashan Jr Acute IL-3 Priming Up-Regulates the Stimulus-Induced Raf-1-Mek-Erk Cascade Independently of IL-3-Induced Activation of Erk J. Immunol., September 1, 2005; 175(5): 3006 - 3014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mack, M. A. Schneider, C. Moll, J. Cihak, H. Bruhl, J. W. Ellwart, M. P. Hogarth, M. Stangassinger, and D. Schlondorff Identification of Antigen-Capturing Cells as Basophils J. Immunol., January 15, 2005; 174(2): 735 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gessner, K. Mohrs, and M. Mohrs Mast Cells, Basophils, and Eosinophils Acquire Constitutive IL-4 and IL-13 Transcripts during Lineage Differentiation That Are Sufficient for Rapid Cytokine Production J. Immunol., January 15, 2005; 174(2): 1063 - 1072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Belvisi Regulation of Inflammatory Cell Function by Corticosteroids Proceedings of the ATS, November 1, 2004; 1(3): 207 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Sloane, N. Tedla, M. Awoniyi, D. W. MacGlashan Jr, L. Borges, K. F. Austen, and J. P. Arm Leukocyte immunoglobulin-like receptors: novel innate receptors for human basophil activation and inhibition Blood, November 1, 2004; 104(9): 2832 - 2839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Min, M. Prout, J. Hu-Li, J. Zhu, D. Jankovic, E. S. Morgan, J. F. Urban Jr., A. M. Dvorak, F. D. Finkelman, G. LeGros, et al. Basophils Produce IL-4 and Accumulate in Tissues after Infection with a Th2-inducing Parasite J. Exp. Med., August 16, 2004; 200(4): 507 - 517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Vilarino and D. W. MacGlashan Jr Actin cytoskeleton-dependent down-regulation of early IgE-mediated signaling in human basophils J. Leukoc. Biol., May 1, 2004; 75(5): 928 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. MacGlashan Jr. Two Regions of Down-Regulation in the IgE-Mediated Signaling Pathway in Human Basophils J. Immunol., May 15, 2003; 170(10): 4914 - 4925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Genovese, G. Borgia, L. Bjorck, A. Petraroli, A. de Paulis, M. Piazza, and G. Marone Immunoglobulin Superantigen Protein L Induces IL-4 and IL-13 Secretion from Human Fc{varepsilon}RI+ Cells Through Interaction with the {kappa} Light Chains of IgE J. Immunol., February 15, 2003; 170(4): 1854 - 1861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Phillips, W. R. Coward, D. I. Pritchard, and C. R. A. Hewitt Basophils express a type 2 cytokine profile on exposure to proteases from helminths and house dust mites J. Leukoc. Biol., January 1, 2003; 73(1): 165 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. F. Gibbs, K. E. S. Plath, H. H. Wolff, and J. Grabbe Regulation of mediator secretion in human basophils by p38 mitogen-activated protein kinase: phosphorylation is sensitive to the effects of phosphatidylinositol 3-kinase inhibitors and calcium mobilization J. Leukoc. Biol., August 1, 2002; 72(2): 391 - 400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miura, S. Lavens-Phillips, and D. W. MacGlashan Jr. Localizing a Control Region in the Pathway to Leukotriene C4 Secretion Following Stimulation of Human Basophils with Anti-IgE Antibody J. Immunol., December 15, 2001; 167(12): 7027 - 7037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. SCHULMAN Development of a Monoclonal Anti-Immunoglobulin E Antibody (Omalizumab) for the Treatment of Allergic Respiratory Disorders Am. J. Respir. Crit. Care Med., October 15, 2001; 164(8): S6 - 11. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miura, S. S. Saini, G. Gauvreau, and D. W. MacGlashan Jr Differences in Functional Consequences and Signal Transduction Induced by IL-3, IL-5, and Nerve Growth Factor in Human Basophils J. Immunol., August 15, 2001; 167(4): 2282 - 2291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. MacGlashan Jr., H.-Z. Xia, L. B. Schwartz, and J. Gong IgE-regulated loss, not IgE-regulated synthesis, controls expression of Fc{varepsilon}RI in human basophils J. Leukoc. Biol., August 1, 2001; 70(2): 207 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. MacGlashan Jr and S. Lavens-Phillips Characteristics of the free cytosolic calcium timelag following IgE-mediated stimulation of human basophils: significance for the nonreleasing basophil phenotype J. Leukoc. Biol., February 1, 2001; 69(2): 224 - 232. [Abstract] [Full Text]
|
|
|
|
|
|
A. de Paulis, R. De Palma, L. Di Gioia, M. Carfora, N. Prevete, G. Tosi, R. S. Accolla, and G. Marone Tat Protein Is an HIV-1-Encoded {beta}-Chemokine Homolog That Promotes Migration and Up-Regulates CCR3 Expression on Human Fc{epsilon}RI+ Cells J. Immunol., December 15, 2000; 165(12): 7171 - 7179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. H. Falcone, H. Haas, and B. F. Gibbs The human basophil: a new appreciation of its role in immune responses Blood, December 15, 2000; 96(13): 4028 - 4038. [Full Text] [PDF]
|
|
|
|
|
|
K. Y. Larbi, A. R. Allen, F. W. K. Tam, D. O. Haskard, R. R. Lobb, P. M. R. Silva, and S. Nourshargh VCAM-1 has a tissue-specific role in mediating interleukin-4-induced eosinophil accumulation in rat models: evidence for a dissociation between endothelial-cell VCAM-1 expression and a functional role in eosinophil migration Blood, November 15, 2000; 96(10): 3601 - 3609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. MacGlashan Jr and J. T. Schroeder Functional consequences of Fc{varepsilon}RI{alpha} up-regulation by IgE in human basophils J. Leukoc. Biol., October 1, 2000; 68(4): 479 - 486. [Abstract] [Full Text]
|
|
|
|
|
|
K. Miura and D. W. MacGlashan Jr Phosphatidylinositol-3 kinase regulates p21ras activation during IgE-mediated stimulation of human basophils Blood, September 15, 2000; 96(6): 2199 - 2205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. BOUSQUET, P. K. JEFFERY, W. W. BUSSE, M. JOHNSON, and A. M. VIGNOLA Asthma . From Bronchoconstriction to Airways Inflammation and Remodeling Am. J. Respir. Crit. Care Med., May 1, 2000; 161(5): 1720 - 1745. [Full Text]
|
|
|
|
|
|
K. Miura and D. W. MacGlashan Jr. Dual Phase Priming by IL-3 for Leukotriene C4 Generation in Human Basophils: Difference in Characteristics Between Acute and Late Priming Effects J. Immunol., March 15, 2000; 164(6): 3026 - 3034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Patella, G. Florio, A. Petraroli, and G. Marone HIV-1 gp120 Induces IL-4 and IL-13 Release from Human Fc{epsilon}RI+ Cells Through Interaction with the VH3 Region of IgE J. Immunol., January 15, 2000; 164(2): 589 - 595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Oskeritzian, Z. Wang, J. P. Kochan, M. Grimes, Z. Du, H.-W. Chang, S. Grant, and L. B. Schwartz Recombinant Human (rh)IL-4-Mediated Apoptosis and Recombinant Human IL-6-Mediated Protection of Recombinant Human Stem Cell Factor-Dependent Human Mast Cells Derived from Cord Blood Mononuclear Cell Progenitors J. Immunol., November 1, 1999; 163(9): 5105 - 5115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Abbal, P. Jourdan, T. Hori, J. Bousquet, H. Yssel, and J. Pene TCR-mediated activation of allergen-specific CD45RO+ memory T lymphocytes results in down-regulation of cell-surface CXCR4 expression and a strongly reduced capacity to migrate in response to stromal cell-derived factor-1 Int. Immunol., September 1, 1999; 11(9): 1451 - 1462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. de Paulis, G. Minopoli, E. Arbustini, G. de Crescenzo, F. Dal Piaz, P. Pucci, T. Russo, and G. Marone Stem Cell Factor Is Localized in, Released from, and Cleaved by Human Mast Cells J. Immunol., September 1, 1999; 163(5): 2799 - 2808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Durham, S. M. Walker, E.-M. Varga, M. R. Jacobson, F. O'Brien, W. Noble, S. J. Till, Q. A. Hamid, and K. T. Nouri-Aria Long-Term Clinical Efficacy of Grass-Pollen Immunotherapy N. Engl. J. Med., August 12, 1999; 341(7): 468 - 475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Saini, D. W. MacGlashan Jr., S. A. Sterbinsky, A. Togias, D. C. Adelman, L. M. Lichtenstein, and B. S. Bochner Down-Regulation of Human Basophil IgE and FC{epsilon}RI{alpha} Surface Densities and Mediator Release by Anti-IgE-Infusions Is Reversible In Vitro and In Vivo J. Immunol., May 1, 1999; 162(9): 5624 - 5630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miura, J. T. Schroeder, W. C. Hubbard, and D. W. MacGlashan Jr. Extracellular Signal-Regulated Kinases Regulate Leukotriene C4 Generation, But Not Histamine Release or IL-4 Production from Human Basophils J. Immunol., April 1, 1999; 162(7): 4198 - 4206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Patella, A. Giuliano, J.-P. Bouvet, and G. Marone Endogenous Superallergen Protein Fv Induces IL-4 Secretion from Human Fc{epsilon}RI+ Cells Through Interaction with the VH3 Region of IgE J. Immunol., November 15, 1998; 161(10): 5647 - 5655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miura and D. W. M. Jr Expression of Protein Kinase C Isozymes in Human Basophils: Regulation by Physiological and Nonphysiological Stimuli Blood, August 15, 1998; 92(4): 1206 - 1218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-J. Sanz, L. Marinova-Mutafchieva, P. Green, R. R. Lobb, M. Feldmann, and S. Nourshargh IL-4-Induced Eosinophil Accumulation in Rat Skin Is Dependent on Endogenous TNF-{alpha} and {alpha}4 Integrin/VCAM-1 Adhesion Pathways J. Immunol., June 1, 1998; 160(11): 5637 - 5645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Hundley, L. A. Marshall, W. C. Hubbard, and D. W. Macglashan Jr. Characteristics of Arachidonic Acid Generation in Human Basophils: Relationship Between the Effects of Inhibitors of Secretory Phospholipase A2 Activity and Leukotriene C4 Release J. Pharmacol. Exp. Ther., March 1, 1998; 284(3): 847 - 857. [Abstract] [Full Text]
|
|
|
|
|
|
D. MacGlashan Jr, J. McKenzie-White, K. Chichester, B. S. Bochner, F. M. Davis, J. T. Schroeder, and L. M. Lichtenstein In Vitro Regulation of Fc&b.epsi;RIalpha Expression on Human Basophils by IgE Antibody Blood, March 1, 1998; 91(5): 1633 - 1643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Redrup, B. P. Howard, D. W. MacGlashan Jr., A. Kagey-Sobotka, L. M. Lichtenstein, and J. T. Schroeder Differential Regulation of IL-4 and IL-13 Secretion by Human Basophils: Their Relationship to Histamine Release in Mixed Leukocyte Cultures J. Immunol., February 15, 1998; 160(4): 1957 - 1964. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Marsh, J. Neely, D. Breazeale, B Ghosh, L. Freidhoff, E Ehrlich-Kautzky, C Schou, G Krishnaswamy, and T. Beaty Linkage analysis of IL4 and other chromosome 5q31.1 markers and total serum immunoglobulin E concentrations Science, May 20, 1994; 264(5162): 1152 - 1156. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
