The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rico-Vargas, S. A.
Right arrow Articles by Osmond, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rico-Vargas, S. A.
Right arrow Articles by Osmond, D. G.

The Journal of Immunology, Vol 152, Issue 6 2845-2852, Copyright © 1994 by American Association of Immunologists

ARTICLES

c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody

SA Rico-Vargas, B Weiskopf, S Nishikawa and DG Osmond
Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada.

To examine the in vivo role of c-kit receptor in B lymphopoiesis we have evaluated precursor B cell populations expressing c-kit in mouse bone marrow and the effects on B cell genesis of administering a neutralizing anti-c-kit mAb, ACK2. Double immunofluorescence labeling and mitotic arrest were used to examine bone marrow cells from BALB/c mice. Almost one-half of TdT+ cells and one-quarter of B220+ cells coexpressed c-kit, mainly at low intensities, and were actively proliferating in vivo. c-kit+ cells that lacked B lineage markers expressed c-kit in high intensities and entered mitosis at an exceptionally rapid rate. In ACK2-treated mice, erythroid and granulocytic cells were almost completely absent from the bone marrow, whereas, in contrast, B lymphopoiesis was stimulated. Pre-B cells expressing cytoplasmic mu-chains; as well as TdT+B220+ cells before mu expression, were increased two- to fourfold in number and production rate. IgM-bearing B lymphocytes were increased in bone marrow and spleen. The results demonstrate that many early precursor B cells in mouse bone marrow constitutively express c-kit receptor. The failure of ACK2 treatment to block B lymphopoiesis, however, suggests that c-kit receptor function is not essential for precursor B cell development in vivo, but can be replaced by alternative signaling systems. The stimulation of B cell genesis by ACK2 treatment may reflect a conferred advantage in the competition for microenvironmental factors which underlies the balance between B lymphopoiesis and other hemopoietic lineages in vivo.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. Li, J. Quirt, H. Q. Do, K. Lyte, F. Fellows, C. G. Goodyer, and R. Wang
Expression of c-Kit receptor tyrosine kinase and effect on beta-cell development in the human fetal pancreas
Am J Physiol Endocrinol Metab, August 1, 2007; 293(2): E475 - E483.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
M. L. Taylor, D. Sehgal, M. Raffeld, H. Obiakor, C. Akin, R. G. Mage, and D. D. Metcalfe
Demonstration That Mast Cells, T Cells, and B Cells Bearing the Activating Kit Mutation D816V Occur in Clusters within the Marrow of Patients with Mastocytosis
J. Mol. Diagn., November 1, 2004; 6(4): 335 - 342.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. L. Pennock and R. K. Grencis
In vivo exit of c-kit+/CD49dhi/{beta}7+ mucosal mast cell precursors from the bone marrow following infection with the intestinal nematode Trichinella spiralis
Blood, April 1, 2004; 103(7): 2655 - 2660.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
R. Mehr, G. Shahaf, A. Sah, and M. Cancro
Asynchronous differentiation models explain bone marrow labeling kinetics and predict reflux between the pre- and immature B cell pools
Int. Immunol., March 1, 2003; 15(3): 301 - 312.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. J. Payne, K. L. Medina, and P. W. Kincade
Loss of c-kit Accompanies B-Lineage Commitment and Acquisition of CD45R by Most Murine B-Lymphocyte Precursors
Blood, July 15, 1999; 94(2): 713 - 723.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. D. Lyman and S. E. W. Jacobsen
c-kit Ligand and Flt3 Ligand: Stem/Progenitor Cell Factors With Overlapping Yet Distinct Activities
Blood, February 15, 1998; 91(4): 1101 - 1134.
[Full Text] [PDF]

Home page
 BloodHome page
O. P. Veiby, O. J. Borge, A. Martensson, E. X. Beck, A. E. Schade, K. Grzegorzewski, S. D. Lyman, I.-L. Martensson, and S. E.W. Jacobsen
Bidirectional Effect of Interleukin-10 on Early Murine B-Cell Development: Stimulation of flt3-Ligand Plus Interleukin-7-Dependent Generation of CD19- ProB Cells From Uncommitted Bone Marrow Progenitor Cells and Growth Inhibition of CD19+ ProB Cells
Blood, December 1, 1997; 90(11): 4321 - 4331.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
V. C. Broudy
Stem Cell Factor and Hematopoiesis
Blood, August 15, 1997; 90(4): 1345 - 1364.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1994 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1994 by The American Association of Immunologists, Inc. All rights reserved.