The Journal of Immunology, Vol 152, Issue 6 2710-2719, Copyright © 1994 by American Association of Immunologists

ARTICLES

Expression of v-src in T cells correlates with nuclear expression of NF- kappa B

DM Eicher, TH Tan, NR Rice, JJ O'Shea and IC Kennedy
Leukocyte Cell Biology Section, NCI-FCRDC, Frederick, MD 21702-1201.

NF-kappa B is a rapidly inducible transcriptional activator that responds to a variety of signals and influences the expression of many genes involved in the immune response. Protein tyrosine kinases transmit signals from cytokine and immune receptors. Very little information exists linking these two important classes of signaling molecules. We now demonstrate that v-src expression correlates with nuclear expression of a kappa B binding complex similar to that induced by phorbol ester and ionomycin, as detected by electrophoretic mobility shift assay using a variety of kappa B sites. This complex was blocked by the tyrosine kinase inhibitor, herbimycin A. The v-src-induced complex comprised the p50 and p65 components of NF-kappa B, as determined by supershift and immunoblot analysis. As a functional correlate of this finding, transient co-transfection of HIV-1 LTR reporter constructs in a different T cell line demonstrated that v-src activated this promoter in a kappa B-dependent manner. We found that transactivation of the HIV-1 LTR by v-src was more sensitive to mutations of the proximal, rather than the distal, kappa B element. The implications for T cell receptor signaling and HIV-1 gene expression are considered.

This article has been cited by other articles:

				S. Y. Eum, G. B. Rha, B. Hennig, and M. Toborek c-Src Is the Primary Signaling Mediator of Polychlorinated Biphenyl-Induced Interleukin-8 Expression in a Human Microvascular Endothelial Cell Line Toxicol. Sci., July 1, 2006; 92(1): 311 - 320. [Abstract] [Full Text] [PDF]

				M. Yeh, N. M. Gharavi, J. Choi, X. Hsieh, E. Reed, K. P. Mouillesseaux, A. L. Cole, S. T. Reddy, and J. A. Berliner Oxidized Phospholipids Increase Interleukin 8 (IL-8) Synthesis by Activation of the c-src/Signal Transducers and Activators of Transcription (STAT)3 Pathway J. Biol. Chem., July 16, 2004; 279(29): 30175 - 30181. [Abstract] [Full Text] [PDF]

				J. Zhang, P. Ping, T. M. Vondriska, X.-L. Tang, G.-W. Wang, E. M. Cardwell, and R. Bolli Cardioprotection involves activation of NF-{kappa}B via PKC-dependent tyrosine and serine phosphorylation of I{kappa}B-{alpha} Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1753 - H1758. [Abstract] [Full Text] [PDF]

				R. C. X. Li, P. Ping, J. Zhang, W. B. Wead, X. Cao, J. Gao, Y. Zheng, S. Huang, J. Han, and R. Bolli PKCepsilon modulates NF-kappa B and AP-1 via mitogen-activated protein kinases in adult rabbit cardiomyocytes Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H1679 - H1689. [Abstract] [Full Text] [PDF]

				M. W. Wooten, M. L. Seibenhener, K. B. W. Neidigh, and M. L. Vandenplas Mapping of Atypical Protein Kinase C within the Nerve Growth Factor Signaling Cascade: Relationship to Differentiation and Survival of PC12 Cells Mol. Cell. Biol., July 1, 2000; 20(13): 4494 - 4504. [Abstract] [Full Text]

				A. Mukhopadhyay, S. K. Manna, and B. B. Aggarwal Pervanadate-induced Nuclear Factor-kappa B Activation Requires Tyrosine Phosphorylation and Degradation of Ikappa Balpha . COMPARISON WITH TUMOR NECROSIS FACTOR-alpha J. Biol. Chem., March 17, 2000; 275(12): 8549 - 8555. [Abstract] [Full Text] [PDF]

				Y. Abu-Amer, F. P. Ross, K. P. McHugh, A. Livolsi, J.-F. Peyron, and S. L. Teitelbaum Tumor Necrosis Factor-alpha Activation of Nuclear Transcription Factor-kappa B in Marrow Macrophages Is Mediated by c-Src Tyrosine Phosphorylation of Ikappa Balpha J. Biol. Chem., November 6, 1998; 273(45): 29417 - 29423. [Abstract] [Full Text] [PDF]

				U. R. Pendurthi, J. T. Williams, and L. V. M. Rao Inhibition of Tissue Factor Gene Activation in Cultured Endothelial Cells by Curcumin : Suppression of Activation of Transcription Factors Egr-1, AP-1, and NF-{kappa}B Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3406 - 3413. [Abstract] [Full Text]

				T. M. Mahon and L. A. J. O'Neill Studies into the Effect of the Tyrosine Kinase Inhibitor Herbimycin A on NF-kappaB Activation in T Lymphocytes J. Biol. Chem., December 1, 1995; 270(48): 28557 - 28564. [Abstract] [Full Text] [PDF]

				S. Singh and B. B. Aggarwal Activation of Transcription Factor NF-kappaB Is Suppressed by Curcumin (Diferuloylmethane) J. Biol. Chem., October 20, 1995; 270(42): 24995 - 25000. [Abstract] [Full Text] [PDF]

				S. Singh and B. B. Aggarwal Protein-tyrosine Phosphatase Inhibitors Block Tumor Necrosis Factor-dependent Activation of the Nuclear Transcription Factor NF-[IMAGE]B J. Biol. Chem., May 5, 1995; 270(18): 10631 - 10639. [Abstract] [Full Text] [PDF]