The Journal of Immunology, Vol 152, Issue 6 2652-2659, Copyright © 1994 by American Association of Immunologists

ARTICLES

IFN-gamma, TNF, and IL-6 production by vaccinia virus immune spleen cells. An in vitro study

EA Carpenter, J Ruby and IA Ramshaw
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra, ACT.

The production of cytokines by virus immune spleen cells after in vitro restimulation was investigated. Vaccinia virus-primed spleen cells from CBA/H mice were stimulated in vitro with virus-infected UV-irradiated syngeneic cells. Both CD4+ and CD8+ T cell populations proliferated after restimulation. TNF, IL-6, and IFN-gamma were detected within 12 h of restimulation with maximal levels reached by 24 h. Adherent cells were major producers of IL-6 and TNF, whereas IFN-gamma production was dependent on CD4+ T cells and adherent cells. The IFN-gamma response was Ag specific, whereas the production of TNF and IL-6 was not. Stimulation with vaccinia virus constructs encoding IFN-gamma or TNF altered the levels of cytokines produced, in that IFN-gamma expression by stimulator cells led to increased IFN-gamma production, whereas TNF expression by stimulator cells augmented both TNF and IFN-gamma production by responder cells.

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