| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 152, Issue 10 4984-4992, Copyright © 1994 by American Association of Immunologists
ARTICLES |
K Pechhold, D Wesch, S Schondelmaier and D Kabelitz
Department of Immunology, Paul Ehrlich Institute, Langen, Germany.
Purified peripheral blood gamma delta T cells proliferated vigorously in response to killed Mycobacterium tuberculosis (M. tb.) in the presence of PBMC but not in the presence of T cell-depleted (E-) feeder cells. Addition of graded numbers of autologous CD4 T cells to E- feeder cells reconstituted in a dose-dependent fashion the response of V gamma 9-expressing gamma delta T cells to M. tb. IL-2 was identified as the major CD4 T cell-derived helper factor required for gamma delta T cell proliferation after stimulation with M. tb. In addition, neutralizing anti-IFN-gamma but not anti-IFN-alpha Ab inhibited the responsiveness of V gamma 9 T cells, suggesting that endogenously produced IFN-gamma was involved in the activation of gamma delta T cells by M. tb. Although gamma delta T cells could not proliferate on their own in the absence of CD4 T cells (or exogenous IL-2), the appearance of IL-2 receptors (CD25) was triggered in the absence of CD4 T cells. Furthermore, IL-10 strongly inhibited the activation of V gamma 9 T cells among unfractionated PBMC responder cells. Similarly, the responsiveness of purified gamma delta T cells to M. tb. occurring in the presence of CD4 T cells was strongly inhibited by IL-10, whereas the activation occurring in the presence of exogenous IL-2 was not impaired. These results show that interactions with Th1-type CD4 T cells are required for efficient activation of peripheral blood gamma delta T cells by M. tb. In addition, our results have practical implications for creating experimental conditions aimed at identifying V gamma 9-selective (myco)bacterial ligands.
This article has been cited by other articles:
|
|
 |
|
  L. Li and C.-Y. Wu CD4+CD25+ Treg cells inhibit human memory {gamma}{delta} T cells to produce IFN-{gamma} in response to M tuberculosis antigen ESAT-6 Blood, June 15, 2008; 111(12): 5629 - 5636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Thompson and M. J. Rogers Bisphosphonates and {gamma}{delta} T-Cells: New Insights into Old Drugs IBMS BoneKEy, August 1, 2006; 3(8): 5 - 13. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Kabelitz, D. Wesch, E. Pitters, and M. Zoller Characterization of Tumor Reactivity of Human V{gamma}9V{delta}2 {gamma}{delta} T Cells In Vitro and in SCID Mice In Vivo J. Immunol., December 1, 2004; 173(11): 6767 - 6776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Gehring, K. M. Dobos, J. T. Belisle, C. V. Harding, and W. H. Boom Mycobacterium tuberculosis LprG (Rv1411c): A Novel TLR-2 Ligand That Inhibits Human Macrophage Class II MHC Antigen Processing J. Immunol., August 15, 2004; 173(4): 2660 - 2668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lee, K. Choi, M. R. Olin, S.-N. Cho, and T. W. Molitor {gamma}{delta} T Cells in Immunity Induced by Mycobacterium bovis Bacillus Calmette-Guerin Vaccination Infect. Immun., March 1, 2004; 72(3): 1504 - 1511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Van Rhijn, L. H. Van den Berg, C. W. Ang, J. Admiraal, and T. Logtenberg Expansion of human {gamma}{delta} T cells after in vitro stimulation with Campylobacter jejuni Int. Immunol., March 1, 2003; 15(3): 373 - 382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Daubenberger, M. Salomon, W. Vecino, B. Hubner, H. Troll, R. Rodriques, M. E. Patarroyo, and G. Pluschke Functional and Structural Similarity of V{gamma}9V{delta}2 T Cells in Humans and Aotus Monkeys, a Primate Infection Model for Plasmodium falciparum Malaria J. Immunol., December 1, 2001; 167(11): 6421 - 6430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Moretto, B. Durell, J. D. Schwartzman, and I. A. Khan {{gamma}}{{delta}}T Cell-Deficient Mice Have a Down-Regulated CD8+ T Cell Immune Response Against Encephalitozoon cuniculi Infection J. Immunol., June 15, 2001; 166(12): 7389 - 7397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Rhodes, R. G. Hewinson, and H. M. Vordermeier Antigen Recognition and Immunomodulation by {{gamma}}{{delta}} T Cells in Bovine Tuberculosis J. Immunol., May 1, 2001; 166(9): 5604 - 5610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. MacDougall, P. Enders, G. Hatfield, D. C. Pauza, and E. Rakasz V{{gamma}}2 TCR Repertoire Overlap in Different Anatomical Compartments of Healthy, Unrelated Rhesus Macaques J. Immunol., February 15, 2001; 166(4): 2296 - 2302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Rojas, K. N. Balaji, A. Subramanian, and W. H. Boom Regulation of Human CD4+ alpha beta T-Cell-Receptor-Positive (TCR+) and gamma delta TCR+ T-Cell Responses to Mycobacterium tuberculosis by Interleukin-10 and Transforming Growth Factor beta Infect. Immun., December 1, 1999; 67(12): 6461 - 6472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Pellegrin, J.-L. Taupin, M. Dupon, J.-M. Ragnaud, J. Maugein, M. Bonneville, and J.-F. Moreau {gamma}{delta}T cells increase with Mycobacterium avium complex infection but not with tuberculosis in AIDS patients Int. Immunol., September 1, 1999; 11(9): 1475 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Vincent, K. Roessner, T. Sellati, C. D. Huston, L. H. Sigal, S. M. Behar, J. D. Radolf, and R. C. Budd Lyme Arthritis Synovial {gamma}{delta} T Cells Respond to Borrelia burgdorferi Lipoproteins and Lipidated Hexapeptides J. Immunol., November 15, 1998; 161(10): 5762 - 5771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wesch, S. Marx, and D. Kabelitz Monocyte-Dependent Death of Freshly Isolated T Lymphocytes: Induction by Phorbolester and Mitogens and Differential Effects of Catalase J. Immunol., August 1, 1998; 161(3): 1248 - 1256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Li, H. Bassiri, M. D. Rossman, P. Kramer, A. F.-O. Eyuboglu, M. Torres, E. Sada, T. Imir, and S. R. Carding Involvement of the Fas/Fas Ligand Pathway in Activation-Induced Cell Death of Mycobacteria-Reactive Human {gamma}{delta} T Cells: A Mechanism for the Loss of {gamma}{delta} T Cells in Patients with Pulmonary Tuberculosis J. Immunol., August 1, 1998; 161(3): 1558 - 1567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Hoft, R. M. Brown, and S. T. Roodman Bacille Calmette-Guerin Vaccination Enhances Human {gamma}{delta} T Cell Responsiveness to Mycobacteria Suggestive of a Memory-Like Phenotype J. Immunol., July 15, 1998; 161(2): 1045 - 1054. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
